A5016735058- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Plant Reproductive Biology
- Fungal Biology and Applications
- Protist diversity and phylogeny
- Plant-Microbe Interactions and Immunity
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Lichen and fungal ecology
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Microbial Natural Products and Biosynthesis
- Fermentation and Sensory Analysis
- Plant biochemistry and biosynthesis
- Fungal Plant Pathogen Control
- Bacterial Genetics and Biotechnology
- Mitochondrial Function and Pathology
- Legume Nitrogen Fixing Symbiosis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fluid Dynamics and Turbulent Flows
Institut de Biologie Intégrative de la Cellule
2017-2025
Université Paris-Saclay
2007-2025
Centre National de la Recherche Scientifique
2015-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2025
CEA Paris-Saclay
2016-2025
Université Paris-Sud
2008-2024
Laboratoire de Microbiologie et Génétique Moléculaires
2014
Expression Génétique Microbienne
1992-2013
Université Paris Cité
1985-2007
Laboratoire de Mécanique des Fluides de Lille - Kampé de Fériet
2007
Abstract Background The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions sexual development. Results We present 10X draft sequence P. genome, linked the sequences large expressed tag collection. Similar higher eukaryotes, transcription/splicing machinery generates numerous non-conventional transcripts. Comparison genome orthologous gene set with one its close relatives, Neurospora crassa ,...
While the extent and impact of horizontal transfers in prokaryotes are widely acknowledged, their importance to eukaryotic kingdom is unclear thought by many be anecdotal. Here we report multiple recent a huge genomic island between Penicillium spp. found food environment. Sequencing two leading filamentous fungi used cheese making, P. roqueforti camemberti, comparison with penicillin producer rubens reveals 575 kb long roqueforti--called Wallaby--present as identical fragments at...
Domestication is an excellent model for studies of adaptation because it involves recent and strong selection on a few, identified traits [1Lin T. Zhu G. Zhang J. Xu X. Yu Q. Zheng Z. Lun Y. Li S. Wang et al.Genomic analyses provide insights into the history tomato breeding.Nat. Genet. 2014; 46: 1220-1226Crossref PubMed Scopus (550) Google Scholar, 2Bosse M. Megens H.J. Frantz L.A.F. Madsen O. Larson Paudel Duijvesteijn N. Harlizius B. Hagemeijer Crooijmans R.P. Groenen M.A. Genomic analysis...
Abstract Among crop fruit trees, the apricot ( Prunus armeniaca ) provides an excellent model to study divergence and adaptation processes. Here, we obtain nearly 600 Armeniaca genomes four high-quality assemblies anchored on genetic maps. Chinese European apricots form two differentiated gene pools with high diversity, resulting from independent domestication events distinct wild Central Asian populations, subsequent flow. A relatively low proportion of genome is affected by selection....
Background Fungal mating types in self-incompatible Pezizomycotina are specified by one of two alternate sequences occupying the same locus on corresponding chromosomes. One sequence is characterized a gene encoding an HMG protein, while hallmark other protein with α1 domain showing similarity to Matα1p Saccharomyces cerevisiae. DNA-binding proteins ubiquitous and well characterized. In contrast, have limited distribution their evolutionary origin obscure, precluding complete understanding...
High-mobility group (HMG) B proteins are eukaryotic DNA-binding characterized by the HMG-box functional motif. These transcription factors play a pivotal role in global genomic functions and control of genes involved specific developmental or metabolic pathways. The filamentous ascomycete Podospora anserina contains 12 genes. Of these, four have been previously characterized; three mating-type that fertilization development fruit-body, whereas last one encodes factor mitochondrial DNA...
Pseudo-homothallism is a reproductive strategy elected by some fungi producing heterokaryotic sexual spores containing genetically different but sexually compatible nuclei. This lifestyle appears as compromise between true homothallism (self-fertility with predominant inbreeding) and complete heterothallism (with exclusive outcrossing). However, pseudohomothallic species face the problem of maintaining mycelia to fully benefit from this lifestyle, homokaryons are self-sterile. Here, we...
Meiosis is the cellular program by which a diploid cell gives rise to haploid gametes for sexual reproduction. Meiotic progression depends on tight physical and functional coupling of recombination steps at DNA level with specific organizational features meiotic-prophase chromosomes. The present study reveals that every step this mediated single molecule: Asy2/Mer2. We show Mer2, identified so far only in budding fission yeasts, fact evolutionarily conserved from fungi...
Summary Transposable elements belonging to the recently identified IS 200 / 605 family radically differ from classical insertion sequences in their transposition mechanism by strictly requiring single‐stranded DNA substrates. This includes encoding only transposase ( TnpA ), and others, like Dra 2 D einococcus radiodurans , which contain a second gene, tnpB dispensable for of unknown function date. Here, we show that TnpB has an inhibitory effect on excision steps transposition. action was...
A central feature of meiosis is pairing homologous chromosomes, which occurs in two stages: coalignment axes followed by installation the synaptonemal complex (SC). Concomitantly, recombination complexes reposition from on-axis association to SC region. We show here that, fungus Sordaria macrospora, this critical transition mediated robust interaxis bridges that contain an axis component (Spo76/Pds5), DNA, plus colocalizing Mer3/Msh4 proteins and Zip2-Zip4 mediator complex....
Abstract In wild-type crosses of the filamentous ascomycete Podospora anserina, after fertilization, only nuclei opposite mating type can form dikaryons that undergo karyogamy and meiosis, producing biparental progeny. To determine role played by in these steps, four mat genes were mutagenized vitro introduced into a strain deleted for its locus. Genetic cytological analyses mutant strain, crossed to each other wild type, showed mating-type information is required recognition nuclear...
Abstract DNAs that encode the mating-type functions (mat+ and mat-) of filamentous fungus Podospora anserina were cloned with use A probe from Neurospora crassa. Cloning full mat information was ascertained through gene replacement experiments. Molecular functional analyses haploid transformants carrying both mating types lead to several striking conclusions. Mat+ mat- strains are dual maters. However, resident is dominant added by transformation respect fruiting body development ascus...
ABSTRACT We cloned the pheromone precursor genes of Podospora anserina in order to elucidate their role biology this fungus. The mfp gene encodes a 24-amino-acid polypeptide finished by CAAX motif, characteristic fungal lipopeptide precursors similar -factor Saccharomyces cerevisiae . mfm 221-amino-acid polypeptide, which is related S. α-factor and contains two 13-residue repeats assumed correspond mature pheromone. deleted coding sequence replacement. mutations specifically affect male...
Background Mating-type loci in yeasts and ascomycotan filamentous fungi (Pezizomycotina) encode master transcriptional factors that play a critical role sexual development. Genome-wide analyses of mating-type-specification circuits mating-type target genes are available Saccharomyces cerevisiae Schizosaccharomyces pombe; however, no such have been performed heterothallic (self-incompatible) Pezizomycotina. The fungus Podospora anserina serves as model for understanding the basic features...
Abstract The Venturia genus comprises fungal species that are pathogens on Rosaceae host plants, including V. inaequalis and asperata apple, aucupariae sorbus pirina pear. Although the genetic structure of populations has been investigated in detail, genomic features underlying these subdivisions remain poorly understood. Here, we report whole genome sequencing 87 strains represent each population within inaequalis. We present a PacBio assembly for EU-B04 reference isolate. size selected...
Abstract Background Despite a growing number of investigations on early diverging fungi, the corresponding lineages have not been as extensively characterized Ascomycota or Basidiomycota ones. The Mucor genus, pertaining to one these is an exception. To this date, restricted annotated genomes publicly available and mainly correspond reference species, circinelloides, medically relevant species. However, genus composed large ubiquitous species well few that reported specifically occur in...