A5064247998- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Insects and Parasite Interactions
- Mollusks and Parasites Studies
- Parasite Biology and Host Interactions
- Asthma and respiratory diseases
- Adipokines, Inflammation, and Metabolic Diseases
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Biochemical Analysis and Sensing Techniques
- Long-Term Effects of COVID-19
- Parasites and Host Interactions
- Insect and Arachnid Ecology and Behavior
- Helminth infection and control
- Folate and B Vitamins Research
- Regulation of Appetite and Obesity
- Malaria Research and Control
- Dermatology and Skin Diseases
- Insect and Pesticide Research
- Adipose Tissue and Metabolism
- Nutrition and Health in Aging
- Mosquito-borne diseases and control
- Vitamin D Research Studies
- Nutrition, Genetics, and Disease
- Parasitic Infections and Diagnostics
Mahidol University
2014-2023
Siriraj Hospital
2014-2023
Health & Life (Taiwan)
2023
National Science and Technology Development Agency
2022
Chulalongkorn University
2019-2020
Institute of Parasitology
2013
Mahidol Oxford Tropical Medicine Research Unit
1993
Periplaneta americana is the predominant cockroach (CR) species and a major source of indoor allergens in Thailand. Nevertheless, data on nature molecular characteristics its allergenic components are rare. We conducted this study to identify characterize P. protein. A random heptapeptide phage display library monoclonal antibody (MAb) specific component previously shown be an molecule were used MAb-bound mimotope phylogenic distribution. Two-dimensional gel electrophoresis, liquid...
Periodic monitoring of Staphylococcus aureus characteristics in a locality is imperative as their drug-resistant variants cause treatment problem. In this study, antibiograms, prevalence toxin genes ( sea-see, seg-ser, seu, tsst-1, eta, etb , and etd ), PFGE types, accessory gene regulator agr ) groups, ability to form biofilm 92 S. Thailand clinical isolates were investigated. They classified into 10 drug groups: groups 1–7 (56 isolates) methicillin resistant (MRSA) 8–10 (36 sensitive...
Abstract Highly diversified astigmatic mites comprise many medically important human household pests such as house dust causing ∼1–2% of all allergic diseases globally; however, their evolutionary origin and diverse lifestyles including reversible parasitism have not been illustrated at the genomic level, which hampers allergy prevention our exploration these pests. Using six high-quality assembled annotated genomes, this study only refuted monophyly ticks, but also thoroughly explored...
Vespa affinis (Asian wasp, Thai banded tiger or local name: Tor Hua Seua) causes the most frequent incidence of medically important Hymenoptera sting in South and Southeast Asia. However, data on venom components attributable to derived-clinical manifestations (local reactions, IgE mediated-anaphylaxis, systemic envenomation) are lacking. This study provides first set information V. proteome, allergenome, reactivity individual components. From 2DE-gel based-proteomics, revealed 93 protein...
This study investigated levels of serum homocysteine, vitamin B12, folic acid, B6 and C, in 37 male 112 female overweight obese Thai volunteers (body mass index; BMI > or = 25.00), 23 90 normal-weight volunteers, who came for a physical check-up at the Out-patient Department, General Practice Section, Rajvithi Hospital, Bangkok from March to October 2000. Data included anthropometric measurements waist/hip ratios. All variables, except height, were significantly higher subjects than normal...
<b><i>Background:</i></b> Cockroach (CR) allergens frequently cause severe asthma in CR-sensitized subjects. Allergen-specific immunotherapy causes a shift of allergic Th2 responses towards Th1 and/or regulatory T cell (Treg) which reduce airway inflammation and prevent disease progression. Data are relatively limited on via CR allergy vaccine. <b><i>Methods:</i></b> The therapeutic efficacy an intranasal liposome-adjuvant vaccine made refined...
Cats (Felis domesticus) are rich source of airborne allergens that prevailed in the environment and sensitized a number people to allergy. In this study, mouse model allergic rhinitis caused by cat was developed for first time used testing therapeutic efficacy novel intranasal liposome-entrapped vaccines made native Fel d 1 (major allergen) comparison with vaccine crude hair extract (cCE). BALB/c mice were cCE mixed alum intraperitoneally intranasally. The treated eight doses either liposome...
One of the most common cockroach types in urban areas, American (Periplaneta americana), has been reported to impose an increased risk allergies and asthma. Limited groups allergens (Per a 1-13) have identified this species due lack genome-related information.To expand allergen profile P. americana, genomic, transcriptomic, proteomic approaches were applied. With support high-quality genome assembled using nanopore, Illumina, Hi-C sequencing techniques, potential based on protein homology....
Abstract Objectives: To investigate the effects of tobacco smoking on serum vitamin B 12 , folic acid and haematological parameters in healthy Thai smokers non-smokers. Design: Cross-sectional study non-smokers a military unit Bangkok, Thailand. Setting: A Subjects: One hundred twenty-three male from who participated voluntarily study, were investigated. Sixty-six same selected as controls. Fasting blood samples collected for investigation variables. Results: The concentration was lower than...
Insect glutathione S-transferases (GSTs) play important roles in insecticide/drug resistance and stress response. Medically, GSTs of house dust mites (Dermatophagoides pteronyssinus Blomia tropicalis) German cockroach (Blattella germanica) are human allergens. In this study, classes, isoforms B-cell allergenic epitopes GST American cockroach, Periplaneta americana, the predominant species tropics subtropics were investigated for first time. Enzymatically active native recombinant P....
Dermatophagoides farinae mite is a predominant source of indoor allergens causing high incidence allergy worldwide. People with different genetic background respond differently to the components, and thus component-resolved diagnosis (CRD) preferred conventional test based on crude extract. In this study, proteome culprit components in D. whole body extract that sensitized allergic patients were studied by using SDS-PAGE (1DE) 2DE-IgE immunoblotting followed LC-MS/MS database search for...
Allergen-specific immunotherapy (AIT) facilitates long-term resolution of allergic morbidity resulting in reduced drug use and increased refractoriness to new sensitization. AIT effectiveness has been demonstrated seasonal perennial allergies, insect stings. However, data studies relative cockroach (CR) allergy are relatively scarce. In this study, mice American CR (Periplaneta americana) were treated with a liposome (L)-entrapped vaccine made mouse Tregitope289-Per 9 the CR,...
The serological diagnosis of human infection with Angiostrongylus cantonensis remains problematic because there are no commercially available validated tests. Most laboratories use domestically prepared tests such as the enzyme-linked immunosorbent assay (ELISA) or immunoblotting. Since laboratory facilities not always in endemic areas, we developed and assessed a rapid lateral flow immunochromatographic (AcQuickDx Test) to detect anti-A. antibodies serum.The test device was assembled...
Genetic variants of the POMC and PCSK1 genes cause severe obesity among patients in early stages childhood.This family-based study analyzed links between single nucleotide polymorphisms (SNPs) either or obesity, as well obesity-related traits obese Thai children their families.The rs1042571 rs6713532 gene a sample 83 family members were investigated using polymerase chain reaction (PCR)-restriction ©FUNPEC-RP www.funpecrp.com.brGenetics Molecular Research 14 (3): 9136-9144 (2015) fragment...