A5016998114- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Insects and Parasite Interactions
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Dermatology and Skin Diseases
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Ecology and Conservation Studies
- Entomological Studies and Ecology
- Olfactory and Sensory Function Studies
- Freshwater macroinvertebrate diversity and ecology
- Insect Utilization and Effects
- Study of Mite Species
- Environmental DNA in Biodiversity Studies
- Lichen and fungal ecology
- Food Quality and Safety Studies
- Urticaria and Related Conditions
- Mast cells and histamine
- Subterranean biodiversity and taxonomy
- Hemoglobinopathies and Related Disorders
- Animal and Plant Science Education
- Drug-Induced Adverse Reactions
- Plant Reproductive Biology
- Mosquito-borne diseases and control
Yonsei University
2015-2024
Yonsei University Health System
2024
ENT and Allergy
2016-2017
Sungkyunkwan University
2013
Medical University of Vienna
2013
Vall d'Hebron Hospital Universitari
2013
Research Institute for Tropical Medicine
2012
Severance Hospital
2009
Inje University Seoul Paik Hospital
2005-2006
CHA University
2004
The house-dust mite (HDM), commonly found in human dwellings, is an important source of inhalant and contact allergens. In this report, the importance HDM allergy Korea characteristics allergens from dust are reviewed with emphasis on investigations performed Korea. Korea, Dermatophagoides farinae dominant species HDM, followed by D. pteronyssinus. Tyrophagus putrescentiae also but its role respiratory allergic disease controversial. relatively low densities populations concentrations major...
Background: Cockroach infestation may sensitize and elicit allergic responses to genetically predisposed individuals. Invertebrate tropomyosins are a frequent cause of allergy highly cross‐reactive in nature. In this study, we aimed produce recombinant German cockroach tropomyosin investigate its allergenicity. Methods: (Bla g 7) was cloned by reverse transcriptase polymerase chain reaction (RT‐PCR). The cDNA over‐expressed Escherichia coli purified affinity chromatography using...
Measurement of IgE specific to purified house dust mite (HDM) allergens may improve allergy diagnosis. This study aimed investigate the sensitization profiles Korean HDM allergic subjects suffering from respiratory and atopic dermatitis (AD) Der f 1, 2, 6, 8, 10, 20. Recombinant were produced in Pichia pastoris (Der 1) or Escherichia coli (5 allergens). reactivity individual recombinant total extract was assessed by ELISA. 1 recognized 79.1%, 2 6 9.3%, 8 6.2%, 10 20 6.6% patients' sera...
Allergenic Characterization of 27-kDa Glycoprotein, a Novel Heat Stable Allergen, from the Pupa Silkworm, Bombyx mori Boiled silkworm pupa is traditional food in Asia, and patients with allergy are common these regions.Still now only one allergen silkworm, arginine kinase, has been identified.The purpose this study was to identify novel allergens by analyzing protein extract after heat treatment.Heat treated extracts were examined proteomic analysis.A glycoprotein identified, expressed...
Abstract Highly diversified astigmatic mites comprise many medically important human household pests such as house dust causing ∼1–2% of all allergic diseases globally; however, their evolutionary origin and diverse lifestyles including reversible parasitism have not been illustrated at the genomic level, which hampers allergy prevention our exploration these pests. Using six high-quality assembled annotated genomes, this study only refuted monophyly ticks, but also thoroughly explored...
<b><i>Background:</i></b> Cockroaches produce potent allergens, and cockroach feces are known to be especially rich in allergens. In this study, we analyze the allergenic components from evaluate allergenicity of recombinant α-amylase identified fecal extract. <b><i>Methods:</i></b> IgE-reactive proteins German extract were analyzed by proteomic analysis immunoblotting. Recombinant was produced its evaluated ELISA....
Abstract Background Several studies have demonstrated that allergen‐specific immunotherapy (SIT) can be an effective treatment for atopic dermatitis (AD). However, there is no relevant mouse model to investigate the mechanism and validate novel modality of SIT in AD. Methods NC/Nga mice with induced AD‐like skin lesions received a subcutaneous injection (an extract house dust mite Dermatophagoides farinae [ DfE ]) or placebo 5 weeks). Clinical histological improvements were examined. The...
Summary Background Various allergenic proteins are produced by house dust mites ( HDM ). However, the allergenicity and clinical implications of these allergens unknown. Objective The purpose this study was to identify in Dermatophagoides farinae elucidate sensitization profiles Korean patients suffering from respiratory (allergic rhinitis and/or asthma) atopic dermatitis symptoms. Methods IgE reactivities sera 160 allergy were analysed one‐ two‐dimensional gel electrophoresis...
While the incidence of patients with atopic dermatitis (AD) march (AM) showing respiratory allergy is steadily rising, pathomechanism still unknown. There are currently no biomarkers to predict progression AM. To explore mechanism AM, AD and AM healthy controls were recruited RNA microarray, flow cytometry, quantitative real-time polymerase chain reaction, immunofluorescence staining performed. We also co-cultured dendritic cells CD4+T various Dermatophagoides farinae allergen fractions....
Summary Background Cockroaches have been known as a cause of respiratory allergies such asthma. IL‐8 plays an integral role in the coordination and persistence inflammatory process chronic inflammation airways Objective We investigated mechanism by which German cockroach extract (GCE) triggers release from human airway epithelial cells. Methods Chemical inhibitors were pretreated before addition GCE for promoter activity protein synthesis IL‐8. The Transcriptional was analysed mutational,...
The ant species Pachycondyla chinensis, which has spread from Far Eastern Asia to New Zealand and North America, induces anaphylactic reactions in human with its sting. However, the major allergens of P. chinensis have not yet been characterized.We selected seven patients histories anaphylaxis induced by chinensis. Two-dimensional electrophoresis (2-DE) was used identify allergens. We subsequently performed Western blots for chinensis-specific IgEs, N-terminal amino acid sequencing,...
and a substantial variability in skin prick test reactivity have been described the literature.In addition, manufacturers use different allergen units to label allergenic activities of their products.This makes it difficult compare products from companies. 8The selection source material for extract preparation is critical factor affecting quality extracts used clinical research.In this study, we prepared standardized house dust mite cultured mites that are indigenous Korea.Contents major...
Histamine H2 receptor antagonists are commonly prescribed medications and known to be well tolerated. However, 99 cases of ranitidine-induced anaphylaxis occurred in Korea from 2007 2014.The purpose this study was determine the incidence, clinical features, diagnostic methods for anaphylaxis.Ranitidine-related pharmacovigilance data 2014 were reviewed. Adverse drug reactions with causal relationships selected, manifestations, outcomes, drug-related information assessed. For further...
Silkworm pupae are widely consumed in Asian countries and allergic reactions following consumption have been described. However, false‑positive responses skin prick allergy tests or non‑specific immunoglobulin E (IgE) to total extract of silkworm pupa make diagnosis difficult. Although improved is required, molecular characterization allergens has not performed date, except for Bomb m 1, an arginine kinase. This study aimed evaluate the allergenicity tropomyosin, a well‑established...
Abstract Background House dust mite (HDM) is a well‐known cause of asthma. Allergen‐specific immunotherapy (AIT) can only modify the natural course disease. Conventional routes HDM AIT are subcutaneous or sublingual. Subcutaneous (SCIT) has disadvantage systemic hypersensitive reaction, and sublingual local allergic reaction low drug adherence. Objective To overcome weak points conventional AIT, we developed loaded biodegradable microneedle patch (MNP) for transdermal (TDIT). We aim to...
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The PROTIA™ Allergy-Q® enzyme immunoassay (EIA) is a recently developed screening assay for specific immunoglobulin E (sIgE) multiple allergens. ImmunoCAP® fluorescent EIA (FEIA) system the most widely used method sIgE detection. In this study, we evaluated performance of compared to system. We 2 systems using sera from 260 Korean allergy patients suffering asthma (26.5%), allergic rhinitis (42.3%), atopic dermatitis (67.7%), and food (18.1%). sIgE-measurement results 7 inhalant allergens, 5...
Group-5 and group-21 allergens, produced by house dust mites storage are 36.6-55.8% identical in their sequences recognized at least 50% of immunoglobulin (Ig)E from the sera individuals allergic to mites. In present study, recombinant group-5 ‑21 allergens three mite species, Dermatophagoides farinae (rDer f 5 21), Tyrophagus putrescentiae (rTyr p Blomia tropicalis (rBlo t were purified Escherichia coli, IgE reactivities cross‑reactivities these allergen variants assessed. The binding...
Cross-Reactivity between Oak and Birch Pollens in Korean Tree PollinosisOak birch trees belong to Fagales order.Specific IgE pollen allergens of both are frequently found Korea pollinosis patients.Oak which comprise 40% forest area common Korea.However, sparse.We compared the allergenicity extracts white oak, sawtooth Mongolian oaks prevalent species Korea, with extract birch.The cross-reactivity four was examined pooled sera 12 patients by ELISA, immunoblotting CAP inhibitions.A protein 17...