A5025954789- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- CRISPR and Genetic Engineering
- Allergic Rhinitis and Sensitization
- COVID-19 Clinical Research Studies
- Asthma and respiratory diseases
- Food Allergy and Anaphylaxis Research
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- COVID-19 and Mental Health
Siriraj Hospital
2018-2023
Mahidol University
2018-2023
Health & Life (Taiwan)
2023
National Science and Technology Development Agency
2022
George Washington University
2018-2019
CRISPR/Cas9-based genome editing has yet to be reported in species of the Platyhelminthes. We tested this approach by targeting omega-1 (ω1) Schistosoma mansoni as proof principle. This secreted ribonuclease is crucial for Th2 polarization and granuloma formation. Schistosome eggs were exposed Cas9 complexed with guide RNA complementary ω1 electroporation or transduction lentiviral particles. Some also transfected a single stranded donor template. Sequences amplicons from gene-edited...
Allergen-specific immunotherapy (AIT) facilitates long-term resolution of allergic morbidity resulting in reduced drug use and increased refractoriness to new sensitization. AIT effectiveness has been demonstrated seasonal perennial allergies, insect stings. However, data studies relative cockroach (CR) allergy are relatively scarce. In this study, mice American CR (Periplaneta americana) were treated with a liposome (L)-entrapped vaccine made mouse Tregitope289-Per 9 the CR,...
Allergen-specific-immunotherapy (ASIT) can cause long-term resolution of allergic diseases, reduces drug use and chances new allergen sensitization. Nevertheless, therapeutic vaccine data on ASIT efficacy for cockroach (CR) allergy are relatively scarce. In this study, mechanism a novel intranasal consisting liposome (L)-entrapped mixture American CR (Periplaneta americana) major (Per 9) immunosuppressive protein Brugia malayi nematode named transforming growth factor-beta homologue (TGH) in...
Many patients develop post-acute COVID syndrome (long (LC)). We compared the immune response of LC and individuals with post-COVID full recovery (HC) during Omicron pandemic. Two hundred ninety-two confirmed infections from January to May 2022 were enrolled. observed anti-SARS-CoV-2 receptor-binding domain immunoglobulin G, surrogate virus neutralization test, T cell subsets, neutralizing antibodies against Wuhan, BA.1, BA.5 viruses (NeuT). NeuT was markedly reduced BA.1 in HC groups, while...
Abstract CRISPR/Cas9 based genome editing has yet been reported in parasitic or indeed any species of the phylum Platyhelminthes. We tested this approach by targeting omega-1 (ω1) Schistosoma mansoni as a proof principle. This secreted ribonuclease is crucial for Th2 priming and granuloma formation, providing informative immuno-pathological readouts programmed editing. Schistosome eggs were either exposed to Cas9 complexed with synthetic guide RNA (sgRNA) complementary exon 6 ω1...
ABSTRACT BACKGROUND In a number of patients, post-acute COVID syndrome develops after acute infection with severe respiratory coronavirus 2 (SARS-CoV-2) (Long [LC]). Here, we examined the immune responses and clinical characteristics individuals LC compared to age- gender-matched healthy recovered (HC) during Omicron pandemic. Immune following BNT162b2 (Pfizer) booster are also determined. METHODS This retrospective cohort study included 292 patients (LC, 158; HC, 134) confirmed have...