A5084025531- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Trace Elements in Health
- Helminth infection and control
- Cytomegalovirus and herpesvirus research
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- Extracellular vesicles in disease
- HIV/AIDS Research and Interventions
- Liver Disease and Transplantation
- MicroRNA in disease regulation
- Drug-Induced Hepatotoxicity and Protection
George Washington University
2015-2020
Children's National
2016
CRISPR/Cas9-based genome editing has yet to be reported in species of the Platyhelminthes. We tested this approach by targeting omega-1 (ω1) Schistosoma mansoni as proof principle. This secreted ribonuclease is crucial for Th2 polarization and granuloma formation. Schistosome eggs were exposed Cas9 complexed with guide RNA complementary ω1 electroporation or transduction lentiviral particles. Some also transfected a single stranded donor template. Sequences amplicons from gene-edited...
Infection with the food-borne liver fluke Opisthorchis viverrini is principal risk factor (IARC Working Group on Evaluation of Carcinogenic Risks to Humans, 2012) for cholangiocarcinoma (CCA) in Lower Mekong River Basin countries including Thailand, Lao PDR, Vietnam and Cambodia. We exploited this link explore role secreted growth termed granulin (Ov-GRN-1) pre-malignant lesions by undertaking programmed CRISPR/Cas9 knockout Ov-GRN-1 gene from genome. Deep sequencing amplicon libraries...
Allogeneic hematopoietic stem cell transplantation (HSCT) can potentially cure human immunodeficiency virus (HIV) by eliminating infected recipient cells, particularly in the context of technologies that may confer HIV resistance to these cells. But, date, Berlin patient remains only case despite multiple attempts eradicate infection with HSCT. One approach improve this is administer virus-specific T a strategy has proven success preventing other infections after transplantation. Although we...
Crosstalk between malignant and neighboring cells contributes to tumor growth. In East Asia, infection with the liver fluke is a major risk factor for cholangiocarcinoma (CCA). The Opisthorchis viverrini secretes growth termed granulin, homologue of human progranulin, which significantly biliary tract fibrosis morbidity. Here, extracellular vesicle (EV)-mediated transfer mRNAs from cholangiocytes naïve recipient was investigated following exposure granulin. To minimize influence endogenous...
The prevalence of nonalcoholic fatty liver disease (NAFLD) is increasing worldwide, including in regions where helminth infections such as the fish-borne fluke Opisthorchis viverrini (Ov) also occur. We investigated effects a high-fat and high-fructose (HFF) diet on development progression NAFLD experimental opisthorchiasis. Two groups hamsters were infected with Ov for 4 months before experiment to induce chronic inflammation. One these (OvHFF) was fed HFF up further months. uninfected...
Complications arising from infection with the carcinogenic liver fluke Opisthorchis viverrini cause substantial morbidity and mortality in Thailand adjacent lower Mekong countries. In parallel, incidence rate of diabetes mellitus (DM) is increasing this same region, indeed worldwide. Many residents opisthorchiasis-endemic regions also exhibit DM, but hepatobiliary disease during co-occurrence these two conditions remains to be characterized. Here, histopathological profile opisthorchiasis DM...
Abstract Infections with several flatworm parasites represent group 1 biological carcinogens, i.e. definite causes of cancer. Infection the food-borne liver fluke Opisthorchis viverrini cholangiocarcinoma (CCA). Whereas causative agent for most cancers, including CCA in West, remains obscure, principal risk factor Thailand is opisthorchiasis. We exploited this established link to explore role secreted parasite growth termed granulin ( Ov -GRN-1) pre-malignant lesions biliary tract. targeted...
Abstract CRISPR/Cas9 based genome editing has yet been reported in parasitic or indeed any species of the phylum Platyhelminthes. We tested this approach by targeting omega-1 (ω1) Schistosoma mansoni as a proof principle. This secreted ribonuclease is crucial for Th2 priming and granuloma formation, providing informative immuno-pathological readouts programmed editing. Schistosome eggs were either exposed to Cas9 complexed with synthetic guide RNA (sgRNA) complementary exon 6 ω1...
Background: Adoptive T cell therapy has been successful in boosting viral-specific immunity post-hematopoietic stem transplant (HSCT), preventing viral rebound of CMV and EBV. However, the therapeutic use cells to boost HIV-specific HIV+ patients met with limited success. Despite multiple attempts eradicate HIV infection allogeneic HSCT, Berlin patient remains only case functional cure. Previous infusions have resulted immune escape from single epitope specificity persistence product. Our...