A5032287850- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Ocular Oncology and Treatments
- Cancer-related Molecular Pathways
- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Neurogenesis and neuroplasticity mechanisms
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Corneal Surgery and Treatments
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Developmental Biology and Gene Regulation
- Photoreceptor and optogenetics research
- interferon and immune responses
- Corporate Finance and Governance
- Neuroscience and Neural Engineering
- Cancer, Hypoxia, and Metabolism
- Corporate Insolvency and Governance
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neuropharmacology Research
- Corporate Governance and Law
Christ University
2024
Oil and Natural Gas Corporation (India)
2018-2024
Sankara Nethralaya
2015-2021
University of Nebraska Medical Center
2009-2016
University of Nebraska at Omaha
2010-2011
University of Nebraska–Lincoln
2011
Abstract The direct reprogramming of somatic cells to a pluripotent state holds significant implications for treating intractable degenerative diseases by ex vivo cell therapy. In addition, the reprogrammed can serve as model and discovery drugs genes. Here, we demonstrate that mouse fibroblast induced stem (iPSCs) represent renewable robust source retinal progenitors, capable generating wide range types includes ganglion (RGCs), cone, rod photoreceptors. They respond simulated...
Background Evidence emerging from a variety of approaches used in different species suggests that Müller cell function may extend beyond its role maintaining retinal homeostasis to progenitors the adult retina. Enriched cells vitro or those re-enter cycle response neurotoxin-damage retina vivo display multipotential and self-renewing capacities, cardinal features stem cells. Methodology/Principal Findings We demonstrate Notch Wnt signaling activate through their canonical pathways rare...
Wet age-related macular degeneration (AMD), which accounts for most AMD-related vision loss, is characterized by choroidal neovascularization (CNV). The underlying mechanism of CNV poorly understood, but evidence indicates pathologic recruitment normal angiogenic signaling pathways such as the VEGF pathway. Recent suggests that pathway regulates angiogenesis in concert with Notch signaling. Here, authors examined role backdrop signaling-mediated regulation retinal angiogenesis.Choroid sclera...
In vertebrate retina, histogenesis occurs over an extended period. To sustain the temporal generation of diverse cell types, retinal progenitor cells (RPCs) must self-renew. However, self-renewal and regulation RPCs remain poorly understood. Here, we demonstrate that cell-extrinsic factors coordinate with epigenetic regulator high-mobility group AT-hook 2 (Hmga2) to regulate late (RPCs). We observed a small subset was capable clonal propagation retained multipotentiality parents in presence...
pH/MMP-9 sensitive fluorescent nanoprobes as non-invasive imaging tool for distinguishing tumor-like microenvironments (low pH and upregulated MMP-9) from non-cancerous in 3D microscaffolds.
A colloidal gold-based lateral flow immunoassay was developed for the rapid quantitative detection of Cystatin-C in serum and whole blood. This device has an assay time 15 min, making it a convenient point-of-care diagnostic tool. The quantification range spanning from 0.5 to 7.5 µg/mL, with lower limit at 0.18 µg/mL. To validate its accuracy, test compared standard nephelometric immunoassay, results exhibited robust linear correlation adjusted r
Abstract Antibodies have been extensively used in numerous applications within proteomics-based technologies, requiring high sensitivity, specificity, a broad dynamic range for detection, and precise, reproducible quantification. Seeking alternatives to antibodies due several inherent limitations of is an area active research tremendous importance. Recently, aptamers receiving increasing attention, because they not only all the advantages antibodies, but also unique advantages, such as...
Direct reprogramming of differentiated cells to induced pluripotent stem (iPS) by ectopic expression defined transcription factors (TFs) represents a significant breakthrough towards the use in regenerative medicine (Takahashi and Yamanaka Cell 2006;126:663-676). However, virus-mediated exogenous could be potentially harmful and, therefore, barrier clinical iPS cells. Several approaches, ranging from plasmid-mediated TF introduction recombinant TFs (Yamanaka 2009;137:13-17; Zhou, Wu, Joo et...
It is a global challenge to provide regular retinal screening for all people with diabetes detect sight-threatening diabetic retinopathy (STDR).To determine if circulating biomarkers could be used prioritize type 2 STDR.This cross-sectional study collected data from October 22, 2018, December 31, 2021. All laboratory staff were masked the clinical diagnosis, assigned cohort, and provided database containing data. This was multicenter conducted in parallel 3 outpatient ophthalmology clinics...
Müller glia (MG), the sole glial cells generated by retinal progenitors, have emerged as a viable cellular target for therapeutic regeneration in degenerative blinding diseases, they possess dormant stem cell properties. However, mammalian MG does not display neurogenic potential of their lower vertebrate counterparts, precluding practical clinical use. The answer to this barrier may be found two interlinked processes underlying potential, i.e., activation properties and differentiation...
Abstract Rapid monitoring of the response to treatment in cancer patients is essential predict outcome therapeutic regimen early course treatment. The conventional methods are laborious, time-consuming, subjective and lack ability study different biomolecules their interactions, simultaneously. Since; mechanisms its therapy dependent on molecular interactions not single biomolecules, an assay capable studying as a whole, preferred. Fourier Transform Infrared (FTIR) spectroscopy has become...
Inhibition of the interaction between p53 and HDM2 is an effective therapeutic strategy in cancers that harbor a wild-type protein such as retinoblastoma (RB). Nanoparticle-based delivery molecules has been shown to be advantageous localized delivery, including eye, by overcoming ocular barriers. In this study, we utilized biocompatible gold nanoparticles (GNPs) deliver anti-HDM2 peptide RB cells. Characterization studies suggested GNP-HDM2 was stable biologically relevant solvents had...
Purpose: Vitreoretinal lymphoma (VRL) is the most common intraocular (IOL). This can be either primary or secondary to central nervous system lymphoma. The diagnosis of (PIOL) currently relies on clinical and cytological analysis vitreous subretinal biopsy. Although cases are diagnosed without much issue, limited amount fluid, subjectivity in reporting, special expertise ocular pathology make challenging. MYD88 L265P mutation has been implicated have diagnostic utility PIOL. In this study,...
Retinoblastoma (RB) is the most common paediatric intraocular tumour. Currently, chemotherapy widely used to reduce chance of metastasis as well for vision salvage. The limitations RB include chemoresistance and cytotoxicity. Recently, immunotherapy considered treating chemoresistant cancers. Although, several molecular targets are available in different cancers, we were interested B7H3, it was differentially expressed between retinoblastoma retina our earlier proteomics study. Hence, this...
<b><i>Background:</i></b> The tumour microenvironment (TME) consisting of cells and multiple stromal cell types regulate growth, invasion metastasis. While the concept TME presence cellular components is widely established in cancers, its significance paediatric intraocular malignancy, retinoblastoma (RB), remains unknown. <b><i>Methods:</i></b> study qualitatively identified subtypes RB by immunohistochemistry....
Glaucoma, where the retinal ganglion cells (RGCs) carrying visual signals from retina to centers in brain are progressively lost, is most common cause of irreversible blindness. The management approaches, whether surgical, pharmacological, or neuroprotective do not reverse degenerative changes. stem cell approach replace dead RGCs a viable option but currently faces several barriers, such as lack renewable, safe, and ethical source that functional could establish contacts with bona fide...
Background The study of human lacrimal gland biology and development is limited. Lacrimal tissue damaged or poorly functional in a number disease states including dry eye disease. Development cell based therapies for diseases requires better understanding the gene expression signaling pathways gland. Differential analysis between other embryologically similar tissues may be helpful furthering our development. Methods We performed global using Affymetrix ® arrays. Primary data from laboratory...
Purpose: Age-related macular degeneration (AMD) is one of the leading causes irreversible central vision loss in elderly population. The current study aims to find non-invasive prognostic biomarkers urine specimens AMD patients. Methods: Peripheral blood and samples were collected from 23 controls 61 Genomic DNA was extracted buffy coat peripheral blood. Allele specific PCR used assay SNPs complement factor H (CFH), component 3 (C3). Comparative proteomic analysis early AMD, choroidal...