A5022648184- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- Hippo pathway signaling and YAP/TAZ
- Cellular transport and secretion
- Glaucoma and retinal disorders
- Neuroscience and Neural Engineering
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Zebrafish Biomedical Research Applications
- Retinopathy of Prematurity Studies
- Developmental Biology and Gene Regulation
- bioluminescence and chemiluminescence research
- Cancer-related Molecular Pathways
- Connexins and lens biology
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Neurological and metabolic disorders
- Mitochondrial Function and Pathology
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Congenital heart defects research
- Circadian rhythm and melatonin
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
Doheny Eye Institute
2021-2023
University of California, Los Angeles
2018-2023
Universidad de Salamanca
2011-2021
Instituto de Investigación Biomédica de Salamanca
2012-2021
Instituto de Neurociencias de Castilla y León
2017
Slug (SNAI2), a member of the well-conserved Snail family transcription factors, has multiple developmental roles, including in epithelial-to-mesenchymal transition (EMT). Here, we show that is critical for pathological angiogenesis needed to sustain tumor growth, and transiently necessary normal angiogenesis. We find upregulation angiogenic endothelial cells (EC) regulates an EMT-like suite target genes, suppresses Dll4-Notch signaling thereby promoting VEGFR2 expression. Both EC-specific...
In the mammalian retina, a metabolic ecosystem exists in which photoreceptors acquire glucose from choriocapillaris with help of retinal pigment epithelium (RPE). While photoreceptor cells are primarily glycolytic, exhibiting Warburg-like metabolism, RPE is reliant on mitochondrial respiration. However, ways metabolism affect cellular functions not clear. We first used human cell line, ARPE-19, to examine context differentiation. show that nicotinamide induced rapid differentiation ARPE-19...
Significance Cell nonautonomous toxicity is involved in various neurodegenerations, but mechanistic understanding limited. Here, we provide insight into cellular mechanisms underlying a dominant macular degeneration, which results from mutant ELOVL4 , and represents an unusual case of two separate cell events. We demonstrate that the first event involves RPE phagocytosis photoreceptor disc membranes contain mislocalized protein. show phagosomes are degraded inefficiently, thus introducing to...
The photoreceptor outer segment (OS) is the phototransductive organelle in vertebrate retina. OS tips are regularly ingested and degraded by adjacent retinal pigment epithelium (RPE), offsetting addition of new disk membrane at base OS. This catabolic role RPE essential for health, with defects ingestion or degradation underlying different forms degeneration blindness. Although proteins required tip have been identified, spatiotemporal analysis process live cells lacking; hence, literature...
Melanosomes are motile, light-absorbing organelles that present in pigment cells of the skin and eye. It has been proposed melanosome localization, both melanocytes retinal epithelium (RPE), involves capture from microtubule motors by an unconventional myosin, which dynamically tethers melanosomes to actin filaments. Recent studies with have questioned this cooperative model. Here, we test model RPE imaging associated labeled filaments microtubules, investigating roles different motor...
CRB3 (Crumbs homologue 3), a member of the CRB protein family (homologous to Drosophila Crumbs), is expressed in different epithelium-derived cell types mammals, where it seems be involved regulating establishment and stability tight junctions ciliogenesis. This has been also detected retina, but little known about its localization function this tissue. Our goal here was perform an in-depth study presence mouse retina analyze expression during photoreceptor ciliogenesis plexiform retinal...
Abstract The CRB proteins CRB1, CRB2 and CRB3 are members of the cell polarity complex Crumbs in mammals that together with Scribble Par complexes stablish a variety types. Although many expressed retinal pigment epithelium (RPE) even though mRNA has been detected ARPE-19 cells RPE/Choroid, to date no protein yet found this tissue. To investigate possibility, we generated an antibody specifically recognize mouse demonstrate expression RPE. Confocal analysis shows is restricted apicolateral...
Apicobasal polarity is essential for epithelial cell function, yet the roles of different proteins in its completion not fully understood. Here, we have studied role protein, CRB2, human retinal pigment (RPE) cells during polarization vitro, and mature murine RPE vivo. After establishing a simplified protocol culture fetal cells, temporal sequence expression localization junction these cells. We found that CRB2 plays key tight maintenance as well cycle arrest. In addition, our studies vivo...
Abstract Acquisition of cell polarization is essential for the performance crucial functions, like a successful secretion and appropriate signaling in many tissues, it depends on correct functioning polarity proteins, including Crumbs complex. The CRB CRB1, CRB2 CRB3, identified mammals, are expressed epithelial-derived tissues brain, kidney retina. has ubiquitous expression been detected embryonic brain tissue; but currently there no data regarding its localization adult brain. In our...
Primary cilia exhibit a distinct complement of proteins, including G-protein-coupled receptors (GPCRs) that mediate sensory and developmental signals. The localization GPCRs to the ciliary membrane involves sequences (CLSs), but it is not known how CLSs might relate cilium type. Here, we studied two rhodopsin (RHO)-like GPCRs, somatostatin receptor (SSTR3) RHO, in three types cilia, from inner medullary collecting duct (IMCD3) cells, hTERT-RPE1 cells (possessing pocket cilia), rod...
CRB3 (Crumbs homologue 3), a member of the CRB protein family (homologous to Drosophila Crumbs), is expressed in different epithelium-derived cell types mammals, where it seems be involved regulating establishment and stability tight junctions ciliogenesis.This has been also detected retina, but little known about its localization function this tissue.Our goal here was perform an in-depth study presence mouse retina analyze expression during photoreceptor ciliogenesis plexiform retinal...
Apicobasal polarity is essential for epithelial cell function, and it determined by the expression of three protein complexes: Scribble, Par Crumbs. Both Crumbs complex, promote apical membrane identity. complex composed PALS1, PATJ homologs (CRB) proteins. We have recently described one proteins, CRB2, in retinal pigment (RPE) cells. To better understand this process, we studied role CRB2 human RPE cells during differentiation vitro mouse retina vivo. achieve goal, analyzed temporal...
ABSTRACT Most defects causing retinal degeneration in retinitis pigmentosa (RP) are rod-specific mutations, but the subsequent of cones, which produces loss daylight vision and high-acuity perception, is most debilitating feature disease. To understand better why cones degenerate how cone might be restored, we have made first single-cell recordings light responses from degenerating interneurons after rods died lost their outer-segment disk membranes synaptic pedicles. We show that functional...