A5037404471- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Olfactory and Sensory Function Studies
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Neuroendocrine regulation and behavior
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience of respiration and sleep
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Nitric Oxide and Endothelin Effects
- Biochemical effects in animals
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Educational Technology in Learning
- Physiological and biochemical adaptations
- Pancreatic function and diabetes
- Aquaculture disease management and microbiota
- Stress Responses and Cortisol
- Regulation of Appetite and Obesity
- Circadian rhythm and melatonin
- Birth, Development, and Health
- CAR-T cell therapy research
- Nerve injury and regeneration
Universidad de Salamanca
2012-2024
Instituto de Estudios de Ciencias de la Salud de Castilla y León
2000-2024
Instituto de Investigación Biomédica de Salamanca
2013-2024
Consejo Superior de Investigaciones Científicas
2000-2013
Virginia Commonwealth University Medical Center
2012
Instituto de Neurociencias de Castilla y León
2011
Fundación Juan March
1995
Universidad de La Laguna
1986-1992
Abstract Recently, the zebrafish has been extensively used for studying development of central nervous system (CNS). However, CNS poorly analyzed in adult. The cholinergic/cholinoceptive was by using choline acetyltransferase (ChAT) immunohistochemistry and acetylcholinesterase (AChE) histochemistry brain, retina, spinal cord. AChE labeling more abundant widely distributed than ChAT immunoreactivity. In telencephalon, ChAT‐immunoreactive (ChAT‐ir) cells were absent, whereas AChE‐positive...
The distribution of NADPH-diaphorase (ND) activity was histochemically investigated in the brain frog Rana perezi. This technique provides a highly selective labeling neurons and tracts. In telencephalon, labeled cells are present olfactory bulb, pallial regions, septal area, nucleus diagonal band, striatum, amygdala. Positive surround preoptic infundibular recesses third ventricle. magnocellular suprachiasmatic hypothalamic nuclei contain stained cells. Numerous anterior, lateral central,...
Abstract The distribution of reduced nicotinamide adenine dinucleotide phosphate (NADPH)‐diaphorase activity was histochemically investigated in the Japanese quail brain. This enzyme is now considered responsible for synthesis nitric oxide, a novel neural messenger whose has not been described avian brain until now. histochemical technique provides simple and reliable method staining selected populations neurons throughout In telencephalon several regions showed heavily stained...
Abstract The distribution and morphological characterization of nicotinamide adenine dinucleotide phosphate‐diaphorase (ND)–positive cells fibers in the tench central nervous system was mapped by using a direct histochemical method. This enzyme observed specific cell populations throughout all main divisions brain. In telencephalon, we found strongly labeled olfactory fibers, as well positive area ventralis telencephalic lobes. Positive staining following diencephalic nuclei: nucleus...
Risperidone is an approved antipsychotic drug belonging to the chemical class of benzisoxazole. This has low solubility in aqueous medium and poor bioavailability due extensive first-pass metabolism high protein binding (>90%). Since new strategies improve efficient treatments are needed, we studied efficiency anionic G4.5 PAMAM dendrimers as nanocarriers for this therapeutic drug. To end, explored dendrimer-risperidone complexation dependence on solvent concentration, pH molar relationship....
Brain developmental disorders in humans, including Autism Spectrum Disorders (ASD) and Down's syndrome, have been linked to increased serotonin levels. This work was designed study changes levels the early stages of development with two classes antipsychotic drugs: Risperidone, a drug that blocks dopamine receptors, fluoxetine, reuptake inhibitor. The use drugs is solid choice decrease increase these neurotransmitters their influence on development. parameters will give an idea effects...
Abstract The hedgehog, a macrosomatic insectivore with an extraordinary development of the olfactory structures, has crucial value for any phylogenetic or comparative study in mammals. distribution pattern and morphology NADPH‐diaphorase‐active calbindin D‐28k‐immunoreactive neurons were studied main accessory bulbs hedgehog. NADPH‐diaphorase (ND) staining was carried out by direct histochemical method, D‐28k (CaBP) immunoreaction using monoclonal antibody avidin‐biotin‐immunoperoxidase...
COEXISTENCE of the calcium binding protein calbindin D-28k and NADPH-diaphorase activity was studied in magnocellular secretory nuclei rat hypothalamus using both immunocytochemical histochemical techniques. Coexistence found all considered (supraoptic, paraventricular, circularis fornicals nuclei) with exception hypothalamic area situated between supraoptic paraventricular nuclei. Since stainings are reliable markers, not based upon physiological characteristics at a given moment, our study...
Abstract Neuronal nitric oxide synthase (nNOS) expression can be regulated under natural or experimental conditions. This work aims at elucidating whether the of nNOS its related NADPH‐diaphorase (ND) activity are modified by manipulation normal inputs to neurons. We used olfactory bulbs from two mouse strains, BALB and CD1, because they show divergences in their synapse patterns, these differences affect periglomerular cells, interneurons expressing tyrosine hydroxylase nNOS/ND. The neurons...
Abstract The distribution and morphologic features of calcium‐binding protein‐ (calbindin D‐28k, calretinin, neurocalcin, parvalbumin) immunoreactive elements were studied in the macaque monkey olfactory bulb by using specific antibodies avidin‐biotin‐immunoperoxidase method. A characteristic laminar pattern stained was observed for each marker. Scarce superficial short‐axon cells stellate demonstrated calbindin D‐28k immunoreactivity outer layers, whereas a moderate number...