A5058338392- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Epilepsy research and treatment
- Neurogenesis and neuroplasticity mechanisms
- Traumatic Brain Injury and Neurovascular Disturbances
- MicroRNA in disease regulation
- Axon Guidance and Neuronal Signaling
- Neonatal and fetal brain pathology
- Infectious Encephalopathies and Encephalitis
- Single-cell and spatial transcriptomics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- S100 Proteins and Annexins
- Optical Imaging and Spectroscopy Techniques
- Neonatal Respiratory Health Research
- Stress Responses and Cortisol
- Influenza Virus Research Studies
Instituto Cajal
2015-2025
Universidad de Málaga
2018
Consejo Superior de Investigaciones Científicas
2010-2016
Unidades Centrales Científico-Técnicas
2016
Universidad de Salamanca
2001-2009
Complejo Hospitalario Universitario de Santiago
1999-2000
Fast ripples are a type of transient high-frequency oscillations recorded from the epileptogenic regions hippocampus and temporal cortex epileptic humans rodents. These events presumably reflect hypersynchronous bursting pyramidal cells. However, oscillatory spectral content fast varies 250 to 800 Hz, well above maximal firing frequency most hippocampal neurons. How such generated is therefore unclear. Here, we combine computational simulations with multisite juxtacellular recordings in vivo...
While novel influential concepts in neuroscience bring the focus to local activities generated within a few tens of cubic micrometers brain, we are still devoid appropriate tools record and manipulate pharmacologically neuronal activity at this fine scale. Here designed, fabricated encapsulated microprobes for simultaneous depth recording drug delivery using exclusively polymer SU-8 as structural material. A tetrode- linear-like electrode patterning was combined first time with single double...
Cognitive impairment is a major concern in temporal lobe epilepsy (TLE). While different experimental models have been used to characterize TLE-related cognitive deficits, little known on whether particular deficit more associated with the underlying brain injuries than epileptic condition per se. Here, we look at relationship between pattern of damage and spatial memory deficits two chronic TLE (lithium-pilocarpine, LIP kainic acid, KA) from rat strains (Wistar Sprague-Dawley) using Morris...
Abstract Theta oscillations play a major role in temporarily defining the hippocampal rate code by translating behavioral sequences into neuronal representations. However, mechanisms constraining phase timing and cell-type-specific preference are unknown. Here, we employ computational models tuned with evolutionary algorithms to evaluate of individual CA1 pyramidal cells recorded mice rats not engaged any particular memory task. We applied unbiased hypothesis-free approaches identify effects...
Abstract The primate hippocampus includes the dentate gyrus, cornu ammonis (CA), and subiculum. CA is subdivided into four fields (CA1-CA3, plus CA3h/hilus of gyrus) with specific pyramidal cell morphology connections. Work in non-human mammals has shown that hippocampal connectivity precisely patterned both laminar longitudinal axes. One main handicaps study neuropathological semiology human lack clear borders. aim this was to explore a histochemical segmentation adult hippocampus,...
Episodic memory deficit is a common cognitive disorder in human temporal lobe epilepsy (TLE). However, no animal model of TLE has been shown to specifically replicate this dysfunction, which limited its translational appeal. Here, using task that tests for nonverbal correlates episodic-like rats, we show kainate-treated rats exhibit selective impairment the “what-where-when” while preserving other forms hippocampal-dependent memories. Assisted by multisite silicon probes, recorded from...
Abstract The reactivation of experience-based neural activity patterns in the hippocampus is crucial for learning and memory. These their associated sharp-wave ripples (SWRs) are highly variable. However, this variability missed by commonly used spectral methods. Here, we use topological dimensionality reduction techniques to analyze waveform recorded at pyramidal layer CA1. We show that SWR waveforms distribute along a continuum low-dimensional space, which conveys information about...
Affective symptoms such as anxiety and depression are frequently observed in patients with epilepsy. The mechanisms of comorbidity epilepsy affective disorders, however, remain unclear. Diverse models traditionally used research, including the status epilepticus (SE) model rats, which aimed at generating chronic epileptic animals; implications different SE rat strains emotional behaviors has not been reported. To address this issue, we examined sequelae two temporal lobe (TLE) –...
Recent reports in human demonstrate a role of theta-gamma coupling memory for spatial episodes and lack people experiencing temporal lobe epilepsy, but the mechanisms are unknown. Using multisite silicon probe recordings epileptic rats engaged episodic-like object recognition tasks, we sought to evaluate absence epileptiform activities. Our data reveal specific association between (30-60 Hz) at proximal stratum radiatum CA1 deficits. We targeted microcircuit with novel approach identify...
Understanding brain operation demands linking basic behavioral traits to cell-type specific dynamics of different brain-wide subcircuits. This requires a system classify the operational modes neurons and circuits. Single-cell phenotyping firing behavior during ongoing oscillations in vivo has provided large body evidence on entorhinal–hippocampal function, but data are dispersed diverse. Here, we mined literature search for information regarding phase-timing over 100 hippocampal/entorhinal...
Abstract Cajal-Retzius cells (CRs) are transient neurons, disappearing almost completely in the postnatal neocortex by programmed cell death (PCD), with a percentage surviving up to adulthood hippocampus. Here, we evaluate CR’s role establishment of adult neuronal and cognitive function using mouse model preventing Bax-dependent PCD. CRs abnormal survival resulted impairment hippocampus-dependent memory, associated vivo attenuated theta oscillations enhanced gamma activity dorsal CA1. At...
Seizures have profound impact on synaptic function and plasticity. While kainic acid is a popular method to induce seizures potentially affect plasticity, it can also produce physiological-like oscillations trigger some forms of long-term potentiation (LTP). Here, we examine whether induction LTP altered in hippocampal slices prepared from rats with different sensitivity develop status epilepticus (SE) by systemic injection acid. Rats were treated multiple low doses (5 mg/kg; i.p.) SE...
The proximodistal axis is considered a major organizational principle of the hippocampus. At interface between hippocampus and other brain structures, CA2 apparently breaks this rule. region involved in social, temporal, contextual memory function, but mechanisms remain elusive. Here, we reveal cell-type heterogeneity characteristic expression gradient transcription factor Sox5 within rat. Using intracellular extracellular recordings followed by neurochemical identification single cells,...
Hippocampal sclerosis, the major neuropathological hallmark of temporal lobe epilepsy, is characterized by different patterns neuronal loss. The mechanisms cell-type-specific vulnerability and their progression histopathological classification remain controversial. Using single-cell electrophysiology in vivo immediate-early gene expression, we reveal that superficial CA1 pyramidal neurons are overactive epileptic rodents. Bulk tissue single-nucleus expression profiling disclose...
Coherent neuronal activity in the hippocampal-entorhinal circuit is a critical mechanism for episodic memory function, which typically impaired temporal lobe epilepsy. To better understand how this implemented and degraded condition, we used normal epileptic rats to examine theta accompanying active exploration. Assisted by multisite recordings of local field potentials (LFPs) layer-specific profiling input pathways, provide detailed quantification proximodistal coherence dorsal hippocampus...
This study demonstrates the peculiarities of glial organization optic nerve head (ONH) a fish, tench (Tinca tinca), by using immunohistochemistry and electron microscopy. We employed antibodies specific for macroglial cells: glutamine synthetase (GS), fibrillary acidic protein (GFAP), S100. also used N518 antibody to label new ganglion cells' axons, which are continuously added fish retina, anti-proliferating cell nuclear antigen (PCNA) specifically locate dividing cells. demonstrate...
Abstract Background Although there is a lot information in the literature about genome size fish, high variability among data for same species reported, being mainly related to methodological aspects. Flow cytometry‐based fluorescence measurements of intercalating dyes most attractive approach due its precision, objectivity, speed, and relative simplicity. Methods We analyze DNA content G0/G1 diploid nuclei three teleost ( Carassius auratus , Tinca tinca Danio rerio ) using flow cytometry....
To determine the effects of sepsis on cerebral energy metabolism, cerebrospinal fluid adenosine monophosphate, inosine inosine, adenosine, guanosine, hypoxanthine, xanthine, and urate concentrations were determined by high-performance liquid chromatography neuron-specific enolase levels means an enzyme immunoassay method in 32 children with sepsis, without meningitis, aged between 2 months 13 years, 160 age-matched controls. The septic group had significantly higher than These results...