A5043001329- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Angiogenesis and VEGF in Cancer
- Stress Responses and Cortisol
- Tryptophan and brain disorders
- Cell death mechanisms and regulation
- Epigenetics and DNA Methylation
- Photodynamic Therapy Research Studies
- Sphingolipid Metabolism and Signaling
- Autophagy in Disease and Therapy
- Diet and metabolism studies
- Lipid Membrane Structure and Behavior
- Computational Drug Discovery Methods
- Cancer Cells and Metastasis
University of Pavol Jozef Šafárik
2019-2024
Photodynamic therapy with hypericin (HY-PDT) and hyperforin (HP) could be treatment modalities for colorectal cancer (CRC), but evidence of their effect on angiogenic factors in CRC is missing. Convenient experimental model utilization essential angiogenesis research. Therefore, not only 2D cell models, also 3D models micro-tumors were used compared. The micro-tumor extent interconnection the chorioallantoic membrane (CAM) was determined by histological analyses. presence proliferating cells...
The prevalence of some chronic diseases, such as cancer or neurodegenerative disorders, differs between sexes. Animal models provide an important tool to adopt potential therapies from preclinical studies humans. Laboratory rats are the most popular animals in toxicology, neurobehavioral, research. Our study aimed reveal basic differences blood metabolome (amino acids, biogenic amines, and acylcarnitines) adult male (n = 10) female Wistar rats. Partial least square-discrimination analysis...
The presence of key hypoxia regulators, namely, hypoxia-inducible factor (HIF)-1α or HIF-2α, in tumors is associated with poor patient prognosis. Hypoxia massively activates several genes, including the one encoding BCRP transporter that proffers multidrug resistance to cancer cells through xenobiotic efflux and a determinant side population (SP) stem-like phenotypes. As natural medicine comes fore, it instinctive look for agents possessing powerful features against resistance. Hypericin,...
Skyrin (SKR) is a plant bisanthraquinone secondary metabolite from the Hypericum genus with potential use in anticancer therapy. However, its effect and mechanism of action are still unknown. The negative SKR on HCT 116 HT-29 cancer cell lines hypoxic normoxic conditions was observed. cells were more responsive to treatment as demonstrated by decreased metabolic activity, cellularity accumulation G1 phase. Moreover, an increasing number apoptotic observed after SKR. Based LC-MS comparative...
With a high annual and lifetime prevalence, depression is becoming the leading contributor to global disease burden. During COVID-19 crisis, mood disorders accelerated significantly. Despite growing evidence, precise underlying mechanisms of (DD) remain unknown. When studying DD in humans, there are many uncontrollable factors such as medication history, age patient or living conditions. In this regard, animal models provide an essential step for examining neural circuitry molecular cellular...
Malignant gliomas are one of the most treatment-refractory cancers. Development resistance to chemo- and radiotherapies contributes these tumors’ aggressive phenotypes. Elevated lipid levels in have been reported for last 50 years. However, molecular mechanisms how tumor tissues obtain lipids utilize them not well understood.In our study, 48.6% phosphatidylcholines were significantly changed during an early stage brain cancer females, 66.2% males. As lysophosphatidylcholines 57.1%...
There is an increasing attention to the role that sex/gender plays in health, behavior and outcomes. Even though we know males females are not same, experiments have sometimes been carried out without considering sex scientific research. It essential for scientists clinicians consider differences as one of underlying physiological determinants health disease provide building blocks evidence-based, individualized medicine. Our work aimed reveal sex-associated lipid metabolite levels adult...