Muhammad Afzal

ORCID: 0000-0002-2559-3450
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About
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Research Areas
  • Fungal Biology and Applications
  • Nanoparticles: synthesis and applications
  • Phytochemicals and Antioxidant Activities
  • Graphene and Nanomaterials Applications
  • Agricultural Science and Fertilization
  • Protein Hydrolysis and Bioactive Peptides
  • Antibiotic Resistance in Bacteria
  • Advances in Cucurbitaceae Research
  • Virus-based gene therapy research
  • Essential Oils and Antimicrobial Activity
  • Phytochemistry and Bioactivity Studies
  • Nitrogen and Sulfur Effects on Brassica
  • Research in Cotton Cultivation
  • Molecular Biology Techniques and Applications
  • Antibiotics Pharmacokinetics and Efficacy
  • Heavy Metals in Plants
  • Genomics, phytochemicals, and oxidative stress
  • Ethnobotanical and Medicinal Plants Studies
  • Escherichia coli research studies
  • Psidium guajava Extracts and Applications
  • Plant Toxicity and Pharmacological Properties
  • Genomics and Rare Diseases
  • Microscopic Colitis
  • Viral gastroenteritis research and epidemiology
  • Plant Pathogens and Fungal Diseases

University of Central Punjab
2018-2024

St. Joseph’s University Medical Center
2021

The University of Agriculture, Peshawar
2012-2014

National Institute for Biological Standards and Control
1994-2007

Zinc oxide nanoparticles (ZnO NPs) are the second most prevalent metal oxide, owing to their characteristics of low cost, safe, and easily prepared. ZnO NPs have been found exhibit unique properties which show potential be used in various therapies. Numerous techniques devised for manufacture zinc because it is one nanomaterials that has received major research interest. Mushroom sources proven efficient, ecologically friendly, inexpensive, safe humankind. In current study, an aqueous...

10.3390/molecules28083532 article EN cc-by Molecules 2023-04-17

Abstract L. edodes ( ) is the most consumed mushroom in world and has been well known for its therapeutic potential as an edible medicinal candidate, it contains dietary fibers, vitamins, proteins, minerals, carbohydrates. In current study butanolic extract of was used to form semisolid butanol extract. The aimed explore biometabolites that might have biological activities n -butanol using FT-IR GC–MS LC–MS. synergistic properties bioactive compounds were futher assessed by performing...

10.1038/s41598-024-56421-7 article EN cc-by Scientific Reports 2024-03-08

Herbal products are preferable to synthetic medicines, and the use of traditional medicines is increasing day-by-day. The current study was designed evaluate potentials bioactive compounds from Citrullus colocynthis by performing FTIR, HPLC, GC-MS analyses, which explore good concentration secondary metabolites, such as gallic acid (74.854 ppm), vanillic (122.616 ferulic (101.045 ppm) with considerable bioactivities. Antimicrobial protein estimated SDS-PAGE, ranging 15 70 kDa in all...

10.3390/molecules28041743 article EN cc-by Molecules 2023-02-11

Abstract Conflicting results have been reported on the detection of paramyxovirus transcripts in Paget's disease, and a possible explanation is differences sensitivity RT-PCR methods for detecting virus. In blinded study, we found no evidence to suggest that laboratories failed detect viral had less sensitive assays, did not measles or distemper samples using most assays evaluated. Introduction: There conflicting role persistent infection disease bone (PDB). Some workers detected virus (MV)...

10.1359/jbmr.070103 article EN Journal of Bone and Mineral Research 2007-01-17

Background: Silver nanoparticles (AgNPs) have received great attention in the biomedical field because of their intrinsic therapeutic properties. Nanoparticles synthesized from silver been studied as antimicrobial, antiviral and anticancer agents found particularly an attractive source for development a new advance group antimicrobial agents. Objectives: In present study, were non-pathogenic Bacillus subtilis strain to assess activity. Methods: Different strains spp. selected screened...

10.5812/jjm.91934 article EN Jundishapur Journal of Microbiology 2020-05-30

Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) is a significant nosocomial pathogen, causing serious threats concerning community-wide outbreaks globally, as well in Pakistan. Antimicrobial resistance A. increasing day by day. Objectives: The study aimed to find out the antibiotic (AMR) patterns and evaluate AMR genes clinical isolates from patients admitted surgical Intensive Care units (ICUs) at different hospitals Lahore, Methods: A total of 593 specimens were collected...

10.5812/jjm.113008 article EN Jundishapur Journal of Microbiology 2021-07-20

Abstract Gibberellins (GAs) are well known for plant growth promotion. GAs production by fungi has received little attention, although substantial work been carried out on other aspects of growth-promoting (PGPF). We investigated and capacity an endophytic fungus isolated from the roots soil grown soybean plants. The is reported as producer PGPF first time in this study. Nine isolates were collected soybean, culture filtrates (CFs) obtained their pure cultures screened Waito-C, a dwarf rice...

10.1080/17429145.2011.642013 article EN Journal of Plant Interactions 2012-01-16

Abstract The current study aimed to analyze the stabilization of sunflower oil using white mustard ( Brassica hirta ) leaf extracts as well their antioxidant potential and cytotoxicity. Selected plant leaves were macerated in different solvents such n -hexane, chloroform, ethyl acetate, -butanol, methanol. total flavonoid contents, phenolic activity by DPPH assay, inhibition linoleic acid oxidation also examined. Moreover, was evaluated calculating conjugated dienes, trienes, peroxide value,...

10.1515/chem-2023-0175 article EN cc-by Open Chemistry 2023-01-01

The sporocarps obtained from NARC, Islamabad and local market were utilized as a source of tissue culturing. Initial culturing was done on malt extract agar plates. discs (0.5 cm in diameter) actively growing culture plates three different mushrooms planted the fresh media Malt medium (MEA), Murashige Skoog's (MS) potato dextrose (PDA) medium. Three replicates taken each case. An increase diameter recorded daily. results indicted that mycelial growth Pleorotus ostreatus, varieties sajar,...

10.3923/jbs.2001.1130.1133 article EN Journal of Biological Sciences 2001-11-15

The current study aims to isolate, purify, and characterize the trypsin inhibitor protein from seeds of soya beans, scientifically known as Glycine max. Its were ground, powder was soaked several times using n-hexane. It added phosphate buffer saline (PBS) followed by filtration centrifugation PBS dissolved extract. supernatant subjected ammonium sulfate precipitation about six fractions, 30% 80% prepared. centrifuged pellets obtained each fraction dialyzed run on SDS-PAGE. precipitated...

10.1177/15593258221131462 article EN cc-by-nc Dose-Response 2022-10-01

Medicinal plants have been extensively exploited for their immense pharmacological and immune-supporting potential. Fruit of Citrullus colocynthis has several active secondary metabolites such as phenolics, flavonoids, essential oils that are used in traditional medicines antidiabetic, anti-inflammatory, antioxidant, antimicrobial agents. In this study, phytoconstituents organic fractions (n-hexane, chloroform, ethyl acetate) the methanolic extract C. were analyzed identified by FT-IR, HPLC,...

10.1177/15593258231187357 article EN cc-by-nc Dose-Response 2023-07-01

Brassica species are vulnerable to narrow genetic base due the ignorance of their wild relatives which possess many superior characters. This study was aimed explore diversity in five from U triangle as well relative Eruca sativa. For complete insight diversity, four accessions, each genus along with one collected different geographical locations (exotic and indigenous) were selected. Six yield associated parameters viz., primary branches plant-1, plant height, main raceme length, silique...

10.2298/gensr1402537k article EN Genetika 2014-01-01

Abstract Mushrooms, like other fungi, are unique in the living world They have been assigned to kingdom of Myceteae. Lentinula edodes (shiitake), is second‐largest and most often consumed edible mushroom world. Shiitake possess large capability be used as food it contains carbohydrates, proteins, vitamins, minerals, fibers, fatty acids, amino etc. In current study powdered form semisolid extract selected fractions (Butanol, ethyl acetate, hexane, chloroform aqueous). The aim was explore...

10.1002/slct.202300139 article EN ChemistrySelect 2023-09-21

Background: Dengue virus (DENV) is an RNA belonging to the family Flaviviridae of genus Flavivirus with worldwide distribution. fever caused by any four closely-related serotypes DENV, emerging pandemic-prone viral disease in many regions world. Objectives: The present study aimed determine prevalence dengue genotypes and children aged below 15 years Lahore, Pakistan. Methods: In this study, 112 serum samples were collected from clinically suspected patients March 2017 December 2018 at...

10.5812/jjm.109512 article EN Jundishapur Journal of Microbiology 2021-04-27
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