A5026006462- Viral Infections and Immunology Research
- Autophagy in Disease and Therapy
- RNA regulation and disease
- Mitochondrial Function and Pathology
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Cardiac Fibrosis and Remodeling
- Cardiac Ischemia and Reperfusion
- Adenosine and Purinergic Signaling
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Extracellular vesicles in disease
- Diabetes Treatment and Management
- Kawasaki Disease and Coronary Complications
- Virus-based gene therapy research
- Cardiovascular Function and Risk Factors
- Inflammasome and immune disorders
- Peptidase Inhibition and Analysis
- Coronary Artery Anomalies
- Cardiovascular Effects of Exercise
- Transplantation: Methods and Outcomes
- ATP Synthase and ATPases Research
- Barrier Structure and Function Studies
- RNA Research and Splicing
University of Alabama
2022-2025
The University of Texas at Dallas
2025
Cedars-Sinai Medical Center
2015-2024
Cedars-Sinai Smidt Heart Institute
2015-2024
University of Alabama at Birmingham
2021
San Diego State University
2011-2014
Myogenesis is a crucial process governing skeletal muscle development and homeostasis. Differentiation of primitive myoblasts into mature myotubes requires metabolic switch to support the increased energetic demand contractile muscle. Skeletal specifically shift from highly glycolytic state relying predominantly on oxidative phosphorylation (OXPHOS) upon differentiation. We have found that this phenomenon dramatic remodeling mitochondrial network involving both clearance biogenesis. During...
Coxsackievirus B3 (CVB3), a member of the picornavirus family and enterovirus genus, causes viral myocarditis, aseptic meningitis, pancreatitis in humans. We genetically engineered unique molecular marker, "fluorescent timer" protein, within our infectious CVB3 clone isolated high-titer recombinant stock (Timer-CVB3) following transfection HeLa cells. "Fluorescent protein undergoes slow conversion fluorescence from green to red over time, Timer-CVB3 can be utilized track virus infection...
Aims: We have shown that autophagy and mitophagy are required for preconditioning. While statin's cardioprotective effects well known, the role of autophagy/mitophagy in statin-mediated cardioprotection is not. In this study, we used HL-1 cardiomyocytes mice subjected to ischemia/reperfusion elucidate mechanism cardioprotection. Results: exposed simvastatin 24 h exhibited diminished protein kinase B (Akt)/mammalian target rapamycin (mTOR) signaling, increased activation unc-51-like 1,...
Fluorescent Timer, or DsRed1-E5, is a mutant of the red fluorescent protein, dsRed, in which fluorescence shifts over time from green to as protein matures. This molecular clock gives temporal and spatial information on turnover. To visualize mitochondrial turnover, we targeted Timer matrix with mitochondrial-targeting sequence (coined "MitoTimer") cloned it into tetracycline-inducible promoter construct regulate its expression. Here report characterization this novel reporter for dynamics....
ABSTRACT Coxsackievirus B (CVB) is a common enterovirus that can cause various systemic inflammatory diseases. Because CVB lacks an envelope, it has been thought to be inherently cytolytic, wherein escape from the infected host cell only by causing rupture. In recent years, however, we and others have observed naked viruses, such as CVB, trigger release of infectious extracellular microvesicles (EMVs) contain viral material. This mode cellular suggested allow virus masked adaptive immune...
Extracellular vesicles (EVs) are small membrane-enclosed structures that have gained much attention from researchers across varying scientific fields in the past few decades. Cells secrete diverse types of EVs into extracellular milieu which include exosomes, microvesicles, and apoptotic bodies. These play a crucial role facilitating intracellular communication via transport proteins, lipids, DNA, rRNA, miRNAs. It is well known number viruses hijack several cellular pathways involved EV...
For many decades viral infections have been suspected as ‘triggers’ of autoimmune disease, but mechanisms for how this could occur difficult to establish. Recent studies shown that are commonly associated with myocarditis and other diseases such coxsackievirus B3 (CVB3) SARS-CoV-2 target mitochondria released from cells in mitochondrial vesicles able activate the innate immune response. Studies Toll-like receptor (TLR)4 inflammasome pathway activated by components. Autoreactivity against...
Eukaryotic cells can respond to diverse stimuli by converging at serine-51 phosphorylation on eukaryotic initiation factor 2 alpha (eIF2α) and activate the integrated stress response (ISR). This is a key step in translational control must be tightly regulated; however, persistent eIF2α observed mouse human atheroma. Potent ISR inhibitors that modulate neurodegenerative disorders have been identified. Here, authors evaluated potential benefits of intercepting chronic metabolic inflammatory...
Antimicrobial-resistant and novel pathogens continue to emerge, outpacing efforts contain treat them. Therefore, there is a crucial need for safe effective therapies. Ultraviolet-A (UVA) phototherapy FDA-approved several dermatological diseases but not internal applications. We investigated UVA effects on human cells in vitro, mouse colonic tissue vivo, efficacy against bacteria, yeast, coxsackievirus group B coronavirus-229E. Several virally transfected were exposed series of specific...
Sodium–glucose cotransporter 2 (SGLT2) inhibitors such as empagliflozin are known to reduce the risk of hospitalizations related heart failure irrespective diabetic state. Meanwhile, adverse cardiac remodeling remains leading cause and death in USA. Thus, understanding mechanisms that responsible for beneficial effects SGLT2 is utmost relevance importance. Our previous work illustrated a connection between regulation mitochondrial turnover cellular energetics using short-acting glucagon-like...
Animal studies have demonstrated beneficial effects of therapeutic hypothermia on myocardial function, yet exact mechanisms remain unclear. Impaired autophagy leads to heart failure and mitophagy is important for mitigating ischemia/reperfusion injury. This study aims investigate whether the are due preserved mitophagy. Under general anesthesia, left anterior descending coronary artery 19 female farm pigs was occluded 90 minutes with consecutive reperfusion. 30 after reperfusion, we...
Coxsackievirus B (CVB) is a common human enterovirus that causes systemic infection but specifically replicates to high titers in the pancreas. It was reported certain viruses induce mitochondrial fission support infection. We documented CVB triggers and blocking limits The transient receptor potential channels have been implicated regulating dynamics; namely, heat capsaicin cation channel subfamily V member 1 (TRPV1) contributes depolarization fission. When we transiently warmed HeLa cells...
Coxsackievirus B3 (CVB3) is a significant human pathogen that commonly found worldwide. CVB3 among other enteroviruses, are the leading causes of aseptic meningo-encephalitis which can be fatal especially in young children. How virus gains access to brain poorly-understood, and host-virus interactions occur at blood-brain barrier (BBB) even less-characterized. The BBB highly specialized biological consisting primarily endothelial cells possess unique properties facilitate passage nutrients...
Microbe-induced meningoencephalitis/meningitis is a life-threatening infection of the central nervous system (CNS) that occurs when pathogens are able to cross blood-brain barrier (BBB) and gain access CNS. The BBB consists highly specialized brain endothelial cells exhibit specific properties allow tight regulation CNS homeostasis prevent pathogen crossing. However, during meningoencephalitis/meningitis, fails protect Modeling remains challenge due characteristics these cells. In this...
ABSTRACT Coxsackievirus B3 is a leading cause of viral aseptic meningitis. To gain entry to the central nervous system, it must interact with and disrupt brain endothelial cells blood-brain barrier. Here, we report global transcriptome stem-cell-derived brain-like during coxsackievirus infection.
Coxsackievirus B is a significant human pathogen and leading cause of myocarditis. We others have observed that certain enteroviruses including coxsackievirus infected cells to shed extracellular vesicles containing infectious virus. Recent reports shown vesicle-bound virus can infect more efficiently than free Though microRNAs are differentially regulated in following infection, few been associated with the from cells. Here we report exclusive trafficking specific into viral compared...
Almost 30% of survivors myocardial infarction (MI) develop heart failure (HF), in part due to damage caused by the accumulation dysfunctional mitochondria. Organelle quality control through Parkin-mediated mitochondrial autophagy (mitophagy) is known play a role mediating protection against HF post-ischaemic injury and remodelling subsequent deteriorated myocardium.
Physiological cardiac hypertrophy is a compensatory remodeling of the heart in response to stimuli such as exercise training or pregnancy that reversible and well-tolerated. We previously described how activating transcription factor 6 (ATF6) proteins, ATF6α ATF6β, were required for pathological hemodynamic stress. Here, we examine functional roles both ATF6 proteins context exercise-induced physiological hypertrophy. After 20 days swim training, found differential roles: whole body...