A5100687051- Monoclonal and Polyclonal Antibodies Research
- Force Microscopy Techniques and Applications
- CAR-T cell therapy research
- Reconstructive Surgery and Microvascular Techniques
- thermodynamics and calorimetric analyses
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- T-cell and B-cell Immunology
- Lipid Membrane Structure and Behavior
- Phagocytosis and Immune Regulation
- Elasticity and Material Modeling
- Parasitic Diseases Research and Treatment
- Dysphagia Assessment and Management
- Molecular Junctions and Nanostructures
- Mechanical and Optical Resonators
- Gastrointestinal motility and disorders
- Satellite Communication Systems
- Receptor Mechanisms and Signaling
- Animal Disease Management and Epidemiology
- Parasites and Host Interactions
- Clinical Nutrition and Gastroenterology
- Gastroesophageal reflux and treatments
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
Guiyang Medical University
2022-2025
Case Western Reserve University
2024
Second Affiliated Hospital of Zhejiang University
2021-2022
Zhejiang University
2015-2020
Huazhong University of Science and Technology
2017
China Medical University
2014
UCLA Medical Center
1995
Abstract Naturally evolved T-cell receptors (TCRs) exhibit remarkably high specificity in discriminating non-self antigens from self-antigens under dynamic biomechanical modulation. In contrast, engineered high-affinity TCRs often lose this specificity, leading to cross-reactivity with and off-target toxicity. The underlying mechanism for difference remains unclear. Our study reveals that natural exploit mechanical force form optimal catch bonds their cognate antigens. This process relies on...
Stimulatory immune receptor NKG2D binds diverse ligands to elicit differential anti-tumor and anti-virus responses. Two conflicting degeneracy recognition models based on static crystal structures in-solution binding affinities have been considered for almost two decades. Whether how recognizes discriminates still remain unclear. Using live-cell-based single-molecule biomechanical assay, we characterized the in situ kinetics of interacting with different absence or presence mechanical force....
Immune checkpoint blockade with monoclonal antibodies (mAbs) that target programmed cell death protein-1 (PD-1) has remarkably revolutionized cancer therapy. Their binding kinetics measured by surface plasmon resonance does not always correlate well their immunotherapeutic efficacies, mainly due to the lack of two-dimensional plasma membrane and capability force sensing manipulation. In this regard, based on a more suitable ultra-sensitive biomechanical nanotool, biomembrane probe (BFP), we...
Distal metastasis is the main cause of clinical treatment failure in patients with colon cancer. It now known that invasion and cancer cells precisely regulated by chemical physical factors vivo. However, role extracellular matrix (ECM) stiffness cell (CCCs) remains unclear. Here, bioinformatical analysis suggested a high expression level yes associated protein1 (YAP1) was significantly poor prognosis patients. We further investigated effects polyacrylamide hydrogels different stiffnesses...
Purpose: The objective of this study was to develop a sensitive, specific and rapid approach diagnose hand foot mouth disease (HFMD) for an early treatment by using loop-mediated isothermal amplification (LAMP) technique. Materials Methods: A reverse-transcription (RT-LAMP) detecting EV71 virus developed, the specificity sensitivity RT-LAMP tested, clinical specimens assayed comparing with conventional polymerase chain reaction (RT-PCR) real-time PCR. Results: total 116 from suspected HFMD...
Complex physical cues including two-dimensional membrane environment, dynamic mechanical force, and bioelectric activity inevitably affect receptor functions. Multiplexed single-molecule force spectroscopy (SMFS) techniques with the capability of live-cell measurements are essential to systemically dissect receptor’s functions under complex biophysical regulation. In this review, we summarize recent progress based SMFS specifically focus on biomembrane probe enhanced stability multiplexed...
Abstract Introduction Urethroplasty is an essential component of gender-affirming phalloplasty for transgender individuals who desire the ability to stand void through a neophallus. Objective We demonstrate creation neourethra using radial artery free flap transmasculine with existing neophallus live surgical footage. Methods The patient was 36 year old individual had undergone abdominal 6 months prior, and desired void. Two teams may simultaneously work; one will harvest forearm from...
Abstract Introduction Phalloplasty can alleviate gender dysphoria in transgender individuals. Various techniques exist and selection of phalloplasty technique depends on patient preference, body habitus, surgeon experience. Objective We demonstrate the creation a neophallus using pedicled abdominal flap live surgical footage. Methods The was 19 year old transmasculine individual who had been gender-affirming hormone therapy for 3 years desired to align external appearance with his identity....
The transmembrane receptor-ligand interactions play a vital role in the physiological and pathological processes of living cells, such as immune cell activation, neural synapse formation, or viral invasion into host cells. Mounting evidence suggests that these involve mechanosensing mechanotransduction, which are directly mediated by force-dependent interactions. Some single-molecule force spectroscopy techniques have been applied to investigate kinetics Among these, biomembrane probe (BFP),...
Focus on low signal-to-noise ratio and reliability of terminals with high speed, this paper puts forward the study synchronization algorithm for high-speed terminal OFDM system in space earth integrated network.We build a simulation platform channel, to test performance bit error moving speed as 50km/h, 100km/h 250km/h.The simulations result show that increase have declined, but increasing can improve system.When SNR is higher than 2dB, rate has near performance.Therefore, proposed resist...
Biomembrane force probe (BFP) is a single-molecule biomechanical technique that has been widely used to characterize protein dynamics (e.g., protein-protein interactions and conformational changes), especially suitable for measuring force-regulated receptor-ligand binding kinetics in situ[1-4]. Integrated with various spectroscopies, such as lifetime assay, it become powerful platform systematically many dissociation of great biological significance, which cannot be done traditional solution...