A5069142490- Immune Cell Function and Interaction
- Galectins and Cancer Biology
- Polyamine Metabolism and Applications
- Trauma Management and Diagnosis
- Tracheal and airway disorders
- Trauma and Emergency Care Studies
- Epigenetics and DNA Methylation
- Cardiac Arrest and Resuscitation
- Pleural and Pulmonary Diseases
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Cardiac, Anesthesia and Surgical Outcomes
- Airway Management and Intubation Techniques
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Autophagy in Disease and Therapy
- Ion channel regulation and function
- Aortic aneurysm repair treatments
- Proteoglycans and glycosaminoglycans research
- Cardiac Health and Mental Health
- Vascular Procedures and Complications
- Trypanosoma species research and implications
- Respiratory Support and Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cellular transport and secretion
- Cancer Research and Treatments
Cleveland Clinic Lerner College of Medicine
2023-2024
West Virginia University
2024
East Carolina University
2014-2023
Cleveland Clinic
2023
The transfer of intact mitochondria between heterogeneous cell types has been confirmed in various settings, including cancer. However, the functional implications on tumor biology are poorly understood. Here we show that is a prevalent phenomenon glioblastoma (GBM), most frequent and malignant primary brain tumor. We identified horizontal from astrocytes as mechanism enhances tumorigenesis GBM. This dependent network-forming intercellular connections GBM cells astrocytes, which facilitated...
E-cadherin is crucial for adhesion of cells to each other and thereby development maintenance tissue. While it has been established that N-glycans inside the cell impact level at surface epithelial-derived cells, unclear whether outside control clustering cell-cell border. Here, we demonstrate reduction weakened recruitment retention border, consequently reduced strength interactions. We conclude are tightly linked placement border mediated adhesion.
Glycosylation modulates growth, maintenance, and stress signaling processes. Consequently, altered N-glycosylation is associated with reduced fitness disease. Therefore, expanding our understanding of N-glycans in altering biological processes utmost interest. Herein, clustered regularly interspaced short palindromic repeats/caspase9 (CRISPR/Cas9) technology was employed to engineer a glycosylation mutant Chinese Hamster Ovary (CHO) cell line, K16, which expresses predominantly hybrid type...
The glioblastoma (GBM) microenvironment is enriched in immunosuppressive factors that potently interfere with the function of cytotoxic T lymphocytes. Cancer cells can directly impact immune system, but mechanisms driving these interactions are not completely clear. Here we demonstrate polyamine metabolite spermidine (SPD) elevated GBM tumor microenvironment. Exogenous administration SPD drives aggressiveness an immune-dependent manner pre-clinical mouse models via reduction CD8+ cell...
Abnormal modifications in N-glycosylation processing are commonly associated with neurological disorders, although the impact of specific N-glycans on neuronal excitability is unknown. By replacement complex types hybrid neuroblastoma cells, we provide first study that addresses how distinct N-glycan excitability. Using CRISPR/Cas9 technology, NB_1, a clonal cell line derived from rat cells (NB), was modified to create an mutant line, NB_1 (-Mgat2), which expresses predominantly type...
The glioblastoma microenvironment is enriched in immunosuppressive factors that potently interfere with the function of cytotoxic T lymphocytes. Cancer cells can directly impact immune system, but mechanisms driving these interactions are not completely clear. Here we demonstrate polyamine metabolite spermidine elevated tumor microenvironment. Exogenous administration drives aggressiveness an immune-dependent manner pre-clinical mouse models via reduction CD8+ cell frequency and phenotype....
Objective The “Stop the Bleed” campaign was created to educate laypeople about bleeding control and make kits available in public locations. Unfortunately, previous research has indicated that up half of all cannot effectively apply a tourniquet. purpose this study determine if could tourniquets more with just-in-time training using combined audio-written instructions versus written-only instructions.Methods We conducted prospective randomized comparing application tourniquet simulated arm....
BackgroundPersistent air leak (PAL) is a challenging problem in patients with spontaneous pneumothorax and chronic lung disease who are poor surgical candidates. Conventional management consists of long-term thoracostomy tube placement; however, some cases, unable to leave the hospital because need for continuous negative pressure. We investigated application endobronchial valves (EBVs) whom intervention was contraindicated.MethodsEBVs were placed PALs arising from nonoperative causes....
Abstract Glioblastoma (GBM) interaction with neural cells is critical to its pathobiology. Emerging evidence suggests that GBM form an interconnected network astrocytes, facilitating tumor persistence. Given reports of intercellular transfer mitochondria in ischemic stroke and other pathologic disease states outside the CNS, we hypothesized this facilitates from astrocytes protumorigenic sequelae. Employing transgenic mice intracranial viral vector transductions rats, found TME occurs mouse...