A5019957114- DNA and Nucleic Acid Chemistry
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Ion channel regulation and function
- Plant Virus Research Studies
- Advanced Fluorescence Microscopy Techniques
- Optical Imaging and Spectroscopy Techniques
- Optical Polarization and Ellipsometry
- Erythrocyte Function and Pathophysiology
- Optical measurement and interference techniques
- Cellular transport and secretion
- Cancer-related Molecular Pathways
- HIV/AIDS drug development and treatment
- Optical Coherence Tomography Applications
- Galectins and Cancer Biology
- Neuroblastoma Research and Treatments
- Cell Image Analysis Techniques
- Transgenic Plants and Applications
- Cancer Cells and Metastasis
- Acute Myeloid Leukemia Research
- Proteoglycans and glycosaminoglycans research
- Retinoids in leukemia and cellular processes
- Caveolin-1 and cellular processes
- DNA Repair Mechanisms
East Carolina University
2012-2021
Piedmont International University
2010-2017
The University of Texas MD Anderson Cancer Center
1999-2005
Baylor College of Medicine
1994-2001
University of Alabama at Birmingham
1990-1999
Johns Hopkins University Center for AIDS Research
1990
University of Nevada, Reno
1983-1989
An efficient transgenic eastern white pine (Pinus strobus L.) plant regeneration system has been established using Agrobacterium tumefaciens strain GV3850-mediated transformation and the green fluorescent protein (gfp) gene as a reporter in this investigation. Stable integration of transgenes genome was confirmed by polymerase chain reaction (PCR), Southern blot, northern blot analyses. Transgene expression analysed T-DNA transformants carrying different numbers copies insertions....
Summary. In acute myeloid leukaemia (AML), cell kinetic quiescence has been postulated to contribute drug resistance. As the anti‐apoptotic genes Bcl‐2 and Bcl‐X L have implicated in cycle regulation, we investigated expression of these non‐proliferating (Q) proliferating (P) AML normal CD34 + progenitor cells. Using reverse transcription polymerase chain reaction, were overexpressed Q versus P cells, whereas no difference S Bax was found. Furthermore, /X but not Bcl‐2/Bax ratio higher...
Myeloid cell leukemia 1 protein (MCL1) is an anti-apoptotic that structurally related to Bcl-2. Unlike other Bcl-2 family proteins are constitutively expressed, MCL1 inducibly expressed in cells recently exposed growth and differentiation stimuli. Here, we report the identification of fortilin as a novel MCL1-interacting by screening yeast two-hybrid library with bait. Fortilin specifically interacted both <i>in vitro</i> and<i>in vivo</i>. The intracellular localization was predominantly...
Chronic inflammation leading to pulmonary fibrosis develops in response environmental pollutants, radiotherapy, or certain cancer chemotherapeutic agents. We speculated that lung injury might be mediated by p53, a proapoptotic transcription factor widely implicated the of cells DNA damage. Intratracheal administration bleomycin led caspase-mediated fragmentation characteristic apoptosis. The effects were associated with translocation p53 from cytosol nucleus only alveolar macrophages had...
Kaposi's sarcoma-associated herpesvirus (KSHV) causes sarcoma, primary effusion lymphoma and multicentric Castleman's disease. KSHV infection of cells produces both latent lytic cycles infection. In vivo, the virus is found predominantly in state. vitro, a can be induced KSHV-infected by treating with phorbol ester (TPA). However, exact signalling events that lead to reactivation are still elusive. Here, role demonstrated for B-Raf/MEK/ERK TPA-induced Inhibiting MEK/ERK using MEK-specific...
To design optimal strategies for intracellular delivery of antisense phosphorothioate oligonucleotides, it may be useful to understand their interaction with cellular macromolecules. Nuclear extracts from LOX amelanotic myeloma cells were studied protein binding oligonucleotides using a Southwestern protocol. Multiple nuclear proteins bound the but no detectable was found phosphodiester oligonucleotides. The strongest signals shown by immunoprecipitation nucleolar C23/nucleolin, 110 kDa...
Journal Article Comparative study of 3D morphology and functions on genetically engineered mouse melanoma cells Get access Ying Zhang, Zhang Department Biomedical Engineering, Tianjin University, 300072, China Search for other works by this author on: Oxford Academic Google Scholar Yuanming Feng, Feng Calvin R. Justus, Justus Departments Internal Medicine Oncology, Brody School Medicine, East Carolina Greenville, NC 27834, USA Wenhuan Jiang, Jiang Physics, 27858, Zhigang Li, Li Jun Q. Lu, Lu...
Background Programmed cell death (PCD) is characterized by a sequence of tightly regulated events that result in the activation caspases and internucleosomal DNA cleavage. Late apoptotic such as DNA-strand breaks can be assayed situ end labeling (ISEL) measurement (sub G1) using flow cytometry. Phosphatidylserine (PS) redistribution from inner plasma membrane leaflet to outer leaflet, an early event PCD, detected annexin V (AxV) binding PS. AxV-fluorescein isothiocyanate (FITC) fluorescence...
We previously demonstrated that 3'-azido-3'-deoxythymidine (AZT) inhibits proliferation of human bone marrow progenitor cells in vitro and incorporation AZT into nuclear DNA may be one mechanism responsible for AZT-induced toxicity [Antimicrob. Agents Chemother. 31:452-454 (1987); Mol. Pharmacol. 36:9-14 (1989)]. The present study explores possible genetic mechanisms involved anemia by evaluating the effects on globin gene expression at both transcriptional translational levels butyric...
Glycoconjugates at the cell surface are crucial for cells to communicate with each other and extracellular microenvironment. While it is generally accepted that glycans vectorial biopolymers, their information content unclear. This report provides evidence distinct N-glycan structures influence spatial arrangement of two integral membrane glycoproteins, Kv3.1 E-cadherin, adherent which in turn alter cellular properties. Distinct were generated by heterologous expression these glycoproteins...
ECS-1, a monoclonal antibody (MoAb) raised to cultured human keratinocytes, stains the intercellular glycocalyx with pemphigus-like pattern and recognizes 35-kDa epidermal surface antigen (ESA) on Western blotting of keratinocyte extracts. When ECS-1 MoAb was used screen expression library, unique cDNA identified that predicted 42-kDa globular protein unknown function. This putative ESA conserved between mice humans encoded by gene chromosome 17q11-12 in linkage neurofibromin. Homology...
E-cadherin is crucial for adhesion of cells to each other and thereby development maintenance tissue. While it has been established that N-glycans inside the cell impact level at surface epithelial-derived cells, unclear whether outside control clustering cell-cell border. Here, we demonstrate reduction weakened recruitment retention border, consequently reduced strength interactions. We conclude are tightly linked placement border mediated adhesion.
Estrogen has a variety of neurotrophic effects mediated via different signaling cascades, including ERK and phosphatidylinositol 3-kinase (PI3K) pathways. In this study, we investigated estrogen inhibitors for PI3K applied directly onto the cut sciatic nerve on retrograde labeling lumbar motoneurons. A mix tracer (Fluorogold) 17beta-estradiol, in combination with an antagonist receptors ICI 182,780, inhibitor ERK1/2 pathway (U0126), (LY-294002), or protein synthesis (cycloheximide), was to...
Glycosylation modulates growth, maintenance, and stress signaling processes. Consequently, altered N-glycosylation is associated with reduced fitness disease. Therefore, expanding our understanding of N-glycans in altering biological processes utmost interest. Herein, clustered regularly interspaced short palindromic repeats/caspase9 (CRISPR/Cas9) technology was employed to engineer a glycosylation mutant Chinese Hamster Ovary (CHO) cell line, K16, which expresses predominantly hybrid type...