A5006653015- Nuclear Structure and Function
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Prenatal Screening and Diagnostics
- Virus-based gene therapy research
- Genetic Syndromes and Imprinting
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Glaucoma and retinal disorders
- Nanoparticle-Based Drug Delivery
- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- Chemokine receptors and signaling
- Tissue Engineering and Regenerative Medicine
- Advanced biosensing and bioanalysis techniques
Université Paris Cité
2020-2024
Institut Jacques Monod
2020-2024
Centre National de la Recherche Scientifique
2020-2024
University of Aberdeen
2023
Sorbonne Paris Cité
2023
Université Paris-Panthéon-Assas
2021
University of Connecticut
2020
Abstract Nuclear pore complexes (NPCs) constitute giant channels within the nuclear envelope that mediate nucleocytoplasmic exchange. NPC diameter is thought to be regulated by tension, but how such changes are physiologically linked cell differentiation, where mechanical properties of nuclei remodeled and mechanosensing occurs, remains unstudied. Here we used cryo-electron tomography show NPCs dilate during differentiation mouse embryonic stem cells into neural progenitors. In...
Abstract Prader-Willi syndrome (PWS) is characterized by neonatal hypotonia, developmental delay and hyperphagia/obesity. This disorder caused the absence of paternally expressed gene products from chromosome 15q11–q13. We previously demonstrated that knocking out ZNF274, a Kruppel-associated box-A-domain zinc finger protein capable recruiting epigenetic machinery to deposit H3K9me3 repressive histone modification, can activate expression normally silent maternal allele SNORD116 in neurons...
Besides assembling nuclear pore complexes, the conduits of transport, many nucleoporins also contribute to chromatin organization and gene expression, with critical roles in development pathologies. We previously reported that Nup133 Seh1, two components Y-complex subassembly scaffold, are dispensable for mouse embryonic stem cell viability but required their survival during neuroectodermal differentiation. Here, a transcriptomic analysis revealed regulates subset genes at early stages...
Many cellular processes, ranging from cell division to differentiation, are controlled by nuclear pore complexes (NPCs). However, studying the contributions of individual NPC subunits these processes in vertebrates has long been impeded their complexity and lack efficient genetic tools. Here, we use genome editing mouse embryonic stem cells (mESCs) characterize role structural components, focusing on short arm Y-complex that comprises Nup85, Seh1 Nup43. We show Nup43, although dispensable...
Binocular vision requires the segregation of retinal ganglion cell (RGC) axons extending from retina into ipsilateral and contralateral optic tracts. RGC axon occurs at chiasm, which forms ventral diencephalon midline. Using expression analyses, explants genetically modified mice, we demonstrate that CXCL12 (SDF1) is required for chiasm. expressed by meninges bordering pathway, CXCR4 both ipsilaterally contralaterally projecting RGCs. or potently promoted outgrowth Further, a higher...
ABSTRACT From their essential function in building up the nuclear pore complexes, nucleoporins have expanded roles beyond transport. Hence, contribution to chromatin organization and gene expression has set them as critical players development pathologies. We previously reported that Nup133 Seh1, two components of Y-complex subunit scaffold, are dispensable for mouse embryonic stem cell viability but required survival during neuroectodermal differentiation. Here, a transcriptomic analysis...
Abstract Prader-Willi syndrome (PWS) is characterized by neonatal hypotonia, developmental delay, and hyperphagia/obesity. This disorder caused the absence of paternally-expressed gene products from chromosome 15q11-q13. We previously demonstrated that knocking out ZNF274, a KRAB-domain zinc finger protein capable recruiting epigenetic machinery to deposit H3K9me3 repressive histone modification, can activate expression normally silent maternal allele SNORD116 in neurons derived PWS iPSCs....
Abstract Many cellular processes, ranging from cell division to differentiation, are controlled by nuclear pore complexes (NPCs). However studying contributions of individual NPC subunits these processes in vertebrates has long been impeded their complexity and the lack efficient genetic tools. Here we use genome editing mouse embryonic stem cells (mESCs) characterize role structural components, focusing on short arm Y-complex that comprises Nup85, Seh1 Nup43. We show Nup43, although...