A5013010324- Nuclear Structure and Function
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Virus-based gene therapy research
- Synthesis and biological activity
- Cancer-related Molecular Pathways
- Hedgehog Signaling Pathway Studies
- Bioactive Compounds and Antitumor Agents
- Ubiquitin and proteasome pathways
- Cancer therapeutics and mechanisms
- Synthesis and Biological Evaluation
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Cancer Mechanisms and Therapy
Université Paris Cité
2020-2023
Institut Jacques Monod
2020-2023
Centre National de la Recherche Scientifique
2020-2023
Institute of Molecular Biology and Pathology
2015-2019
Sapienza University of Rome
2015-2018
National Research Council
2016-2018
We designed 39 new 2-phenylindole derivatives as potential anticancer agents bearing the 3,4,5-trimethoxyphenyl moiety with a sulfur, ketone, or methylene bridging group at position 3 of indole and halogen methoxy substituent(s) positions 4-7. Compounds 33 44 strongly inhibited growth P-glycoprotein-overexpressing multi-drug-resistant cell lines NCI/ADR-RES Messa/Dx5. At 10 nM, stimulated cytotoxic activity NK cells. 20-50 arrested >80% HeLa cells in G2/M phase cycle, stable arrest mitotic...
Tubulin-targeting molecules are widely used cancer therapeutic agents. They inhibit microtubule-based structures, including the mitotic spindle, ultimately preventing cell division. The final fates of microtubule-inhibited cells however often heterogeneous and difficult to predict. While recent work has provided insight into response inhibitors microtubule dynamics (taxanes), tubulin polymerization remains less well characterized. Arylthioindoles (ATIs) recently developed inhibitors. We...
Karyopherin beta-1/Importin beta-1 is a conserved nuclear transport receptor, acting in protein import interphase and as global regulator of mitosis. These pleiotropic functions reflect its ability to interact with, regulate, different pathways during the cell cycle, operating major effector GTPase RAN. Importin overexpressed cancers characterized by high genetic instability, an observation that highlights importance identifying partners Here we present first comprehensive profile importin...
Besides assembling nuclear pore complexes, the conduits of transport, many nucleoporins also contribute to chromatin organization and gene expression, with critical roles in development pathologies. We previously reported that Nup133 Seh1, two components Y-complex subassembly scaffold, are dispensable for mouse embryonic stem cell viability but required their survival during neuroectodermal differentiation. Here, a transcriptomic analysis revealed regulates subset genes at early stages...
Protein conjugation with small ubiquitin-related modifier (SUMO) is a post-translational modification that modulates protein interactions and localisation. RANBP2 large nucleoporin endowed SUMO E3 ligase SUMO-stabilising activity, implicated in some cancer types. part of larger complex, consisting SUMO-modified RANGAP1, the GTP-hydrolysis activating factor for GTPase RAN. During mitosis, RANBP2-SUMO-RANGAP1 complex localises to mitotic spindle kinetochores after microtubule attachment. Here,...
Many cellular processes, ranging from cell division to differentiation, are controlled by nuclear pore complexes (NPCs). However, studying the contributions of individual NPC subunits these processes in vertebrates has long been impeded their complexity and lack efficient genetic tools. Here, we use genome editing mouse embryonic stem cells (mESCs) characterize role structural components, focusing on short arm Y-complex that comprises Nup85, Seh1 Nup43. We show Nup43, although dispensable...
ABSTRACT From their essential function in building up the nuclear pore complexes, nucleoporins have expanded roles beyond transport. Hence, contribution to chromatin organization and gene expression has set them as critical players development pathologies. We previously reported that Nup133 Seh1, two components of Y-complex subunit scaffold, are dispensable for mouse embryonic stem cell viability but required survival during neuroectodermal differentiation. Here, a transcriptomic analysis...
Review on KPNB1 (karyopherin (importin) beta 1), with data DNA, the protein encoded, and where gene is implicated.
Abstract Many cellular processes, ranging from cell division to differentiation, are controlled by nuclear pore complexes (NPCs). However studying contributions of individual NPC subunits these processes in vertebrates has long been impeded their complexity and the lack efficient genetic tools. Here we use genome editing mouse embryonic stem cells (mESCs) characterize role structural components, focusing on short arm Y-complex that comprises Nup85, Seh1 Nup43. We show Nup43, although...