A5103087476- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- Nerve injury and regeneration
- MicroRNA in disease regulation
- Hypothalamic control of reproductive hormones
- Neuroscience and Neuropharmacology Research
- Fibroblast Growth Factor Research
- RNA Research and Splicing
- Parkinson's Disease Mechanisms and Treatments
- Photoreceptor and optogenetics research
- Angiogenesis and VEGF in Cancer
- Glaucoma and retinal disorders
- Wnt/β-catenin signaling in development and cancer
- Chemokine receptors and signaling
- Plant Molecular Biology Research
- Intraocular Surgery and Lenses
- Neuroscience of respiration and sleep
- Epigenetics and DNA Methylation
- Plant Reproductive Biology
- Olfactory and Sensory Function Studies
- Connexins and lens biology
- Cellular Mechanics and Interactions
- Neuroblastoma Research and Treatments
- Photochromic and Fluorescence Chemistry
Instituto de Neurociencias
2016-2025
Consejo Superior de Investigaciones Científicas
2016-2025
University of Aberdeen
2023-2025
Hospital Nacional de Parapléjicos
2010-2023
Universitat de Miguel Hernández d'Elx
2016-2022
Servicio de Salud de Castilla La Mancha
2013
The retina of lower vertebrates grows continuously by integrating new neurons generated from progenitors in the ciliary margin zone (CMZ). Whether mammalian CMZ provides neural with retinal cells is controversial. Live imaging embryonic expressing eGFP shows that migrate laterally to where differentiated ganglion (RGCs) reside. Because Cyclin D2, a cell-cycle regulator, enriched ventral CMZ, we analyzed D2−/− mice test whether source cells. Neurogenesis diminished D2 mutants, leading...
Signals from the lens regulate multiple aspects of eye development, including establishment size, patterning presumptive iris and ciliary body in anterior optic cup migration differentiation neural crest cells. To advance understanding molecular mechanism by which regulates we performed transcriptome profiling embryonic chicken retinas after removal. Genes associated with nervous system development were upregulated lens-removed eyes, but region did not adopt a retina identity following...
The existence of axons extending from one retina to the other has been reported during perinatal development in different vertebrates. However, it thought that these are either a labeling artifact or misprojections. Here, we show unequivocally small subset retinal ganglion cells (RGCs) project opposite and guidance receptor Unc5c, expressed region where retinal-retinal (R-R) RGCs located, is necessary sufficient guide retina. In addition, Netrin1, an Unc5c ligand, ventral diencephalon...
Besides giving rise to oligodendrocytes (the only myelin-forming cell in the Central Nervous System (CNS) physiological conditions), Oligodendrocyte Precursor Cells (OPCs) are responsible for spontaneous remyelination after a demyelinating lesion. They present along mouse and human CNS, both during development adulthood, yet how OPC behavior is modified throughout life not fully understood. The activity of adult OPCs still particularly unexplored. Significantly, most molecules involved...
Abstract Axon pathfinding is a key step in neural circuits formation. However, the transcriptional mechanisms regulating its progression remain poorly understood. The binary decision of crossing or avoiding midline taken by some neuronal axons during development represents robust model to investigate that control selection axonal trajectories. Here, identify novel regulators axon guidance, this work compares transcriptome and chromatin occupancy profiles two subpopulations, ipsilateral...
Anosmin-1, defective in Kallmann's syndrome, participates the adhesion, migration and differentiation of different cell types CNS. Although not fully understood, mechanisms action Anosmin-1 involve interaction with proteins, being fibroblast growth factor receptor 1 (FGFR1) modulation its signalling best studied to date. Using glutathione-S-transferase pull-down assays we demonstrate that FnIII.3 (Fibronectin-like type III) domain combination whey acidic protein-FnIII.1, but each them...
Signaling through fibroblast growth factor receptors (FGFRs) is essential for many cellular processes including proliferation and migration, as well differentiation events such myelination. Anosmin-1 an extracellular matrix (ECM) glycoprotein that interacts with the receptor 1 (FGFR1) to exert its biological actions this receptor, although intracellular pathways underlying anosmin-1 signaling remain largely unknown. This protein defective in X-linked form of Kallmann syndrome (KS) has a...
The protein anosmin-1, coded by the KAL1 gene responsible for X-linked form of Kallmann syndrome (KS), exerts its biological effects mainly through interaction with and signal modulation fibroblast growth factor receptor 1 (FGFR1). We have previously shown third fibronectin-like type 3 (FnIII) domain N-terminal region anosmin-1 FGFR1. Here, we demonstrate that missense mutations reported in patients KS, C172R N267K did not alter or substantially reduce, respectively, binding to These...
The development and survival of dopaminergic neurons are influenced by the fibroblast growth factor (FGF) pathway. Anosmin-1 (A1) is an extracellular matrix protein that acts as a major regulator this signaling pathway, controlling FGF diffusion, receptor interaction shuttling. In particular, previous work showed A1 overexpression results in more olfactory bulb. Prompted those intriguing results, study, we investigated effects on different populations catecholaminergic central (CNS)...
Sexual maturation and olfactory bulb defects found in prokineticin 2 (Pk2) receptor (Pkr2) mutant mice resembling the phenotypic characteristics of Kallmann syndrome (KS), gave rise to question whether these genes would have a role KS pathogenesis. Later, mutations both were identified patients suffering from KS. The gene responsible for Xlinked form KS, ANOS1, encodes ECM protein anosmin 1. Among other functions, 1 can regulate activity FGFR1, encoded by one involved autosomal transmission...
Eph/ephrin signaling is crucial for organizing retinotopic maps in vertebrates. Unlike other EphAs, which are expressed the embryonic ventral retina, EphA4 found retinal ganglion cell (RGC) layer at perinatal stages, and its role mammalian visual system development remains unclear. Using classic vitro stripe assays, we demonstrate that, while RGC axons repelled by ephrinB2, they grow on ephrinB1 stripes through EphA4-mediated adhesion. In vivo, from EphA4-deficient mice either sex show...
Binocular vision requires the segregation of retinal ganglion cell (RGC) axons extending from retina into ipsilateral and contralateral optic tracts. RGC axon occurs at chiasm, which forms ventral diencephalon midline. Using expression analyses, explants genetically modified mice, we demonstrate that CXCL12 (SDF1) is required for chiasm. expressed by meninges bordering pathway, CXCR4 both ipsilaterally contralaterally projecting RGCs. or potently promoted outgrowth Further, a higher...
Abstract Neuronal migration is a vital process needed for subsequent assembly and function of neural circuitry during embryonic development. The vast majority progenitors are generated far from their final destination need to migrate considerable distances reach specific cortical layer. Innovations in cell culture techniques fluorescence microscopy now facilitate the direct visualization movements Here, detailed protocol record analyze particular type early migrating neurons, Cajal Retzius...
ABSTRACT Oligodendrocyte precursor cells (OPCs) are responsible for spontaneous remyelination after a demyelinating lesion. They present in large parts of the mouse and human central nervous system, both during development adulthood, yet how their physiological behaviour is modified throughout life remains largely unknown. Moreover, activity adult OPCs still not fully understood. Significantly, most molecules involved OPC-mediated also development, phenomenon that may be clinically relevant....
The existence of axons extending from one retina to the other has been reported during perinatal development in different vertebrates. However, it thought that these are either a labelling artefact or misprojections. Here, we unequivocally show small subset retinal ganglion cells (RGCs) project opposite and guidance receptor Unc5c, expressed region where R-R neurons located, is necessary sufficient guide retina. In addition, Netrin1, an Unc5c ligand, ventral diencephalon pattern consistent...