In cases of pulp exposure due to deep dental caries or severe traumatic injuries, existing pulp-capping materials have a limited ability reconstruct dentin-pulp complexes and can result in pulpectomy because their low potentials accelerate cell activities, such as migration, proliferation, differentiation. Therefore, the development more effective therapeutic agents has been anticipated for direct capping. Dental tissues are enriched with stem cells (DPSCs). Here, authors investigated...

Wnt5a, a non-canonical Wnt protein, is known to play important roles in several cell functions. However, little about the effects of Wnt5a on osteoblastic differentiation periodontal ligament (PDL) cells. Here, we examined and associated intracellular signaling human PDL stem/progenitor cells (HPDLSCs). We found that suppressed expression bone-related genes (ALP, BSP, Osterix) alizarin red-positive mineralized nodule formation HPDLSCs under osteogenic conditions. Immunohistochemical analysis...

Wnt5a, a member of the noncanonical Wnt proteins, is known to play important roles in development various organs and postnatal cell functions. However, little about effects Wnt5a on human periodontal ligament (PDL) cells. In this study, we examined localization potential function PDL tissue. Immunohistochemical analysis revealed that was expressed predominantly rat Semi-quantitative reverse-transcription polymerase chain reaction Western blotting demonstrated cells (HPDLCs) its receptors...

Appropriate mechanical loading during occlusion and mastication play an important role in maintaining the homeostasis of periodontal ligament (PDL) tissue. Connective tissue growth factor (CTGF/CCN2), a matricellular protein, is known to upregulate extracellular matrix production, including collagen PDL However, underlying mechanisms CTGF/CCN2 regulation integrity remain unclear. In this study, we investigated effect on osteo/cementoblastic fibroblastic differentiation human stem cells using...

Periodontal ligament stem cells (PDLSCs) have recently been proposed as a novel option in periodontal regenerative therapy. However, one of the issues is difficulty stably generating PDLSCs because variation cell potential between donors. Here, we show that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properties human (PDL) cells. Sema3A expression was specifically observed dental follicle during tooth development and parts mature PDL tissue rodent tissue. levels were found to be...

The periodontal ligament (PDL) plays an important role in anchoring teeth the bone socket. Damage to PDL, such as after severe inflammation, can be treated with a therapeutic strategy that uses stem cells derived from PDL tissue (PDLSCs), has received intense scrutiny over past decade. However, there is insufficient number of PDLSCs within for treating damage. Therefore, we sought induce differentiation induced pluripotent (iPS) into initial step toward therapy. To this end, first iPS neural...

Objective In this study, we measured the expression of R‐spondin 2 ( RSPO 2) in periodontal ligament PDL ) tissue and cells. Further, examined effects on osteoblastic differentiation immature human cells HPDLC s). Background family proteins are secreted glycoproteins that play important roles embryonic development homeostasis through activation Wnt/β‐catenin signaling pathway. 2, a member family, has been reported to enhance osteogenesis mice. However, little is known regarding tissues....

Periodontal tissue regeneration is important for preserving teeth. ligament stem cells (PDLSCs) are useful in periodontal regeneration, however tooth extraction required to obtain these cells. Therefore, we focused on induced pluripotent (iPS) and established a method PDLSC-like from iPS Specifically, first differentiated into neural crest-like (iNCs). Next, obtained (iPDLSCs) by culturing iNCs extracellular matrix (ECM) derived human primary (HPDLCs). This differentiation suggested that ECM...

Abstract We aimed to evaluate the materials based on 4‐methacryloxyethyl trimellitate anhydride/methyl methacrylate tri‐n‐butylborane (Super‐bond [SB]) and nano hydroxyapatite (naHAp) for repair of perforation at pulp chamber floor (PPF) in vitro vivo models. SB naHAp were mixed mass ratio 10% or 30% produce naHAp/SB. Human periodontal ligament stem cells (HPDLSCs) cultured resin discs naHAp/SB analyze effects cell adhesion, proliferation, cementoblastic differentiation. A rat PPF model was...

Cellular senescence has been suggested to be involved in physiological changes of cytokine production. Previous studies showed that the concentration tumor necrosis factor‐α (TNF‐α) is higher blood aged people compared with young people. So far, precise effects TNF‐α on odontoblastic differentiation pulp cells have controversial. Therefore, we aimed clarify how this affected during aging. Human dental (HDPCs) were cultured until reaching plateau their growth, and isolated at actively (young...

In cases in which dental pulp tissue is accidentally exposed, direct capping often performed to induce reparative dentin formation. Although macrophages are essential for the inflammatory response and repair, emergence pattern role of have not been clarified. Here, we investigated M1/M2 after a effects M2 on odontoblastic differentiation stem cell (DPSC) clones. The was using rat model. Alizarin Red S staining quantitative RT-PCR examine effect mineralization DPSC Immunohistochemical...

Abstract Objective The purpose of this study was to evaluate the function Schwann cells in wound healing periodontal tissue. Background In our previous study, glial cell line‐derived neurotrophic factor (GDNF) promoted migration human ligament (PDL) and that GDNF expression increased wounded reportedly induces precursors. play a crucial role regeneration peripheral tissues, including bone However, on tissue remains unclear. Methods A transwell assay WST‐1 (water‐soluble tetrazolium...

The aim of this study is to clarify the biological functions decorin (DCN) in healing and regeneration wounded periodontal tissue. We investigated expression pattern DCN during tissue rats by immunohistochemistry effects on osteoblastic differentiation human ligament (PDL) stem cells (HPDLSCs) preosteoblasts Alizarin red S staining, quantitative reverse transcription-polymerase chain reactions, western blotting. was increased around PDL day 5 after surgery compared with nonwounded tissue,...

Deep caries and severe periodontitis cause bone resorption in periodontal tissue, leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are important for the healing of defective tissue. It is increasingly understood that tissue mediated through secretion trophic factors, particularly exosomes. This study investigated effects exosomes from human PDLSCs (HPDLSCs-Exo) on osteoblast-like vitro rat calvarial defects vivo. HPDLSCs-Exo were isolated characterized by their particle shape,...

Periodontal ligament (PDL) stem cells (PDLSCs) have been reported as a useful cell source for periodontal tissue regeneration. However, one of the issues is difficulty obtaining sufficient number PDLSCs clinical application because very few can be isolated from PDL donors. Therefore, we aimed to identify specific factor that converts human into stem-like cells. In this study, microarray analysis comparing gene profiles PDLSC lines (2-14 and 2-23) with those line low differentiation potential...

Actin alpha 2, smooth muscle (ACTA2) is an actin isoform that forms the cytoskeleton. plays a crucial role in numerous cellular functions. ACTA2 marker of functional periodontal ligament (PDL) fibroblasts and upregulated by transforming growth factor-β1 (TGF-β1); however, underlying function PDL tissue unknown. We aimed to examine localization potential tissues cells.RNA expression was determined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) quantitative...

Periodontal ligament stem cells (PDLSCs) play central roles in periodontal (PDL) tissue homeostasis, repair, and regeneration. Previously, we established a protocol to differentiate human-induced pluripotent cell-derived neural crest-like (iNCs) into PDLSC-like (iPDLSCs) using human PDL extracellular matrix (ECM). However, it remained unclear what factors principally regulate the differentiation of iNCs iPDLSCs. In this study, aimed identify transcription factor regulating production ECM,...

Background and Objective Human periodontal ligament cells (HPDLCs) express transforming growth factor‐β1 ( TGF ‐β1) that regulates differentiation proliferation, plays key roles in homeostasis of PDL tissue. Transgelin is a cytoskeleton‐associated protein with an Smad‐binding element its gene promoter region. In this study, we examined the localization potential function transgelin tissue cells. Material Methods Microarray analysis HPDLC lines (2‐14, 2‐23 2‐52) was performed. Expression s by...

Dopamine (DA) is produced from tyrosine by hydroxylase (TH). A recent study has reported that DA promotes the mineralization of murine preosteoblasts. However, role in odontoblasts not been examined. Therefore, this investigation, we researched expression TH and effects on differentiation preodontoblasts (KN-3 cells). Immunostaining showed were intensely expressed rat incisors molars. KN-3 cells D1-like D2-like receptors for DA. Furthermore, promoted odontoblastic cells, whereas an...