A5025849406- Receptor Mechanisms and Signaling
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Neuropeptides and Animal Physiology
- Mass Spectrometry Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Simulation-Based Education in Healthcare
- Chemical Synthesis and Analysis
- Clostridium difficile and Clostridium perfringens research
- Enzyme Production and Characterization
- Bacterial Identification and Susceptibility Testing
- Infection Control in Healthcare
- Peptidase Inhibition and Analysis
- Advanced Proteomics Techniques and Applications
- Vehicle Noise and Vibration Control
- Intramuscular injections and effects
- Physical Activity and Health
- Glycogen Storage Diseases and Myoclonus
- Pharmacological Effects and Assays
- Palliative Care and End-of-Life Issues
- Ion channel regulation and function
- Racial and Ethnic Identity Research
- Advanced ceramic materials synthesis
- Synthesis of β-Lactam Compounds
- Microbial Metabolism and Applications
Moderna Therapeutics (United States)
2021-2024
Ewha Womans University
2023
Catholic University of Korea
2014-2022
Duke University Hospital
2014-2018
Duke Medical Center
2014-2018
Semyung University
2017
Korea Research Institute of Chemical Technology
2013
Sangmyung University
2011
National Institute of Diabetes and Digestive and Kidney Diseases
1992
National Institutes of Health
1992
The β2-adrenergic receptor (β2AR) has been a model system for understanding regulatory mechanisms of G-protein-coupled (GPCR) actions and plays significant role in cardiovascular pulmonary diseases. Because all known β-adrenergic drugs target the orthosteric binding site receptor, we set out to isolate allosteric ligands this by panning DNA-encoded small-molecule libraries comprising 190 million distinct compounds against purified human β2AR. Here, report discovery negative modulator...
Significance β-Arrestin regulates G protein-coupled receptor (GPCR) signaling by interacting with two regions of agonist-activated receptors—the phosphorylated C terminus and the seven transmembrane helix bundle. The phosphorylation pattern on GPCRs is thought to be primary driver β-arrestin binding affinity functional consequences. To more effectively delineate relative contributions these interactions, we present an innovative strategy homogeneously phosphorylate purified GPCRs—enzymatic...
A mutant GPCR exhibits G protein–biased signaling because of defective phosphorylation by GRK, not an inability to bind β-arrestin.
Abstract Glycogen Storage Disease 1a (GSD1a) is a rare, inherited metabolic disorder caused by deficiency of glucose 6-phosphatase (G6Pase-α). G6Pase-α critical for maintaining interprandial euglycemia. GSD1a patients exhibit life-threatening hypoglycemia and long-term liver complications including hepatocellular adenomas (HCAs) carcinomas (HCCs). There no treatment the current standard-of-care managing (Glycosade ® /modified cornstarch) fails to prevent HCA/HCC risk. Therapeutic modalities...
TPS3175 Background: The Wnt/β-catenin pathway is a frequently activated oncogenic and well-described driver of many cancers. FOG-001 designed to be first-in-class direct inhibitor β-catenin that blocks β-catenin’s interaction with the T-cell factor (TCF) family transcription factors. Preclinical studies have indicated can cause tumor growth inhibition regression by disrupting β-catenin-dependent signaling. unique from previously reported modulators as it directly inhibit TCF interaction,...
Mussels attach to virtually all types of inorganic and organic surfaces in aqueous environments, catecholamines composed 3,4-dihydroxy-l-phenylalanine (DOPA), lysine, histidine mussel adhesive proteins play a key role the robust adhesion. DOPA is an unusual catecholic amino acid, its side chain called catechol. In this study, we displayed moiety DOPA-histidine on Escherichia coli using outer membrane protein W as anchoring motif for first time. Localization cell surface was confirmed by...
A total of 1921 Escherichia coli isolated from healthy animals (501 cattle, 832 pigs, and 588 chickens) 237 isolates diseased pigs were tested to determine the prevalence apramycin gentamicin resistance in Korea during 2004–2007. Apramycin/gentamicin resistances observed chicken 0.2%/0.6%, 11.2%/13.6%, 0.5%/18.2%, respectively. Gentamicin/apramycin was much higher E. (71/237, 30.0%) than those (93/832, 11.2%). The aminoglycoside gene content all apramycin-gentamicin-resistant (n = 164)...
Maple syrup urine disease (MSUD) is a rare, inherited, metabolic disorder characterized by dysfunction of the multi-subunit, mitochondrial enzyme complex branched-chain alpha-keto acid dehydrogenase (BCKDH). BCKDH catalyzes oxidative decarboxylation amino acids (BCAAs). BCAAs and their neurotoxic intermediates can accumulate in blood tissues absence functional BCKDH. We evaluated lipid nanoparticle (LNP)-based treatment approach to address all possible genetic mutations that cause MSUD...
Purpose The purpose of this study was to identify occurrence needle stick and sharp injuries(NSI) among students, level faculty stress, necessity informed consent when students practice injection skills in fundamentals nursing (FNP). Methods Data were collected using self-reporting questionnaires 74 members who teach FNP responded it. Questionnaires included general characteristics, experiences NSI, stress level, consent. analyzed frequency, percent paired t-test. Results Of members, 51.4%...
Purpose This study was done to understand nursing studentsâ exposure of needlestick and sharp injuries (NSSI), level anxiety as well experience practicing injection with human beings during Fundamentals Nursing (FN) practice. Methods Data consisted self-report questionnaires completed by 571 students who had an practice in FN. The questionnaire included questions on general characteristics, NSSI, injections beings, other experiences. were analyzed using descriptive statistics content...
Purpose: The purpose of this study was to identify the time for young adults reach resting blood pressure after walking as measured by an automatic and a mercury sphygmomanometer. Methods: Participants were 33 nursing students in their 20s. Blood every minute on both arms simultaneously total 6 times 12 minutes treadmill at ratings perceived exertion (RPE) 11. Data analyzed using paired t-test, Bland-Altman plots repeated measures ANOVA. Results: Systolic same measurements corresponding 3 if...
Abstract Genetic and epigenetic alterations in the Wnt signaling pathway leading to constitutive activation of driver oncogene Β-catenin occur at least 20% all human cancers. We have developed conformationally hyperstabilized α-helical peptides (Helicons) that bind directly with picomolar affinity block its interaction TCF transcription factors. describe here characterization anti-tumor efficacy, pharmacodynamic biomarkers mechanism action using multiple vivo patient-derived xenograft (PDX)...
Abstract Wnt signaling pathway mutations leading to constitutive activation of the driver oncogene β-catenin occur in at least 20% all human cancers, but itself has remained undruggable by traditional modalities. In order inhibit activity β-catenin, we have developed conformationally hyperstabilized α-helical peptides (Helicons) that are cellularly permeable and bind directly block its interaction with TCF family transcription factors. Starting a helical peptide derived from phage display,...