A5101832058- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- Spine and Intervertebral Disc Pathology
- Macrophage Migration Inhibitory Factor
- Nuclear Receptors and Signaling
- X-ray Diffraction in Crystallography
- Retinal Diseases and Treatments
- Crystallization and Solubility Studies
- Spinal Fractures and Fixation Techniques
- TGF-β signaling in diseases
- Pelvic and Acetabular Injuries
- Neuroendocrine Tumor Research Advances
- Cancer-related molecular mechanisms research
- Click Chemistry and Applications
- Medical Imaging and Analysis
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Signaling Pathways in Disease
- Toxin Mechanisms and Immunotoxins
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Biomarkers in Disease Mechanisms
- RNA and protein synthesis mechanisms
- Cancer Cells and Metastasis
- Bacterial Genetics and Biotechnology
Xuzhou Medical College
2025
Zhejiang Chinese Medical University
2025
Lishui Central Hospital
2020-2023
Wenzhou Medical University
2020-2023
Zhejiang University
2021-2023
Sir Run Run Shaw Hospital
2021-2023
Lishui University
2021-2022
First Affiliated Hospital of Jinan University
2020
Jinan University
2015
Nanjing Agricultural University
2015
Formononetin (FMN) is a phytoestrogen that belongs to the isoflavone family. It has antioxidant and anti-inflammatory effects, as well as, many other biological activities. Existing evidence aroused interest in its ability protect against osteoarthritis (OA) promote bone remodeling. To date, research on this topic not been thorough issues remain controversial. Therefore, purpose of our study was explore protective effect FMN knee injury clarify possible molecular mechanisms. We found...
Bacillus amyloliquefaciens is a plant-beneficial Gram-positive bacterium involved in suppressing soil-borne pathogens through the secretion of secondary metabolites and high rhizosphere competence. Biofilm formation regarded as prerequisite for In this work, we show that plant extracts affect chemotaxis biofilm B. SQY 162 (SQY 162). All carbohydrates tested induced strain; however, bacterial growth rate was not influenced by addition carbohydrates. A strong chemotactic response were both...
The aceBAK operon was partially induced by a multicopy plasmid which carried the promoter region of gene encodes its repressor, iclR. Gel shift and DNase I analyses demonstrated that IclR binds to own promoter. Disruption iclR increased expression an iclR::lacZ fusion. Although are both regulated IclR, responds carbon source, while does not.
Oxidative stress can alter the expression level of microRNAs (miRNAs) and has a role in oxidative damage generated by reactive oxygen species (ROS). While previous studies have demonstrated that miR‑146a, miR‑21 miR‑150 are essential for ROS production heart disease, these miRNAs spinal cord injuries not yet been examined. The present study focused on examining during H2O2 stimulation rat neuronal (RN‑sc) cells. RN‑sc cells were treated with H2O2, harvested reverse transcription quantitative...
Osteosarcoma (OS) is the most common malignant bone tumor and long‑term survival rates remain unsatisfactory. Transforming growth factor‑β (TGF‑β) has been revealed to play a crucial role in OS progression, RepSox an effective TGF‑β inhibitor. In present study, effect of on proliferation cell lines (HOS 143B) was detected. The results that effectively inhibited cells by inducing S‑phase arrest apoptosis. Moreover, inhibitory migration invasion confirmed wound‑healing Transwell assays....
Bone metabolic homeostasis is largely dependent on the dynamic balance between osteoblasts and osteoclasts. MicroRNAs (miRNAs) play critical roles in regulating bone metabolism. In this study, we explored role of a new miRNA (miR-148a) osteoporosis. We compared phenotype miR-148a knockout (KO) mice wild-type (WT) littermates. found KO exhibited an increased mass decreased osteoclastogenesis to WT group. vitro, overexpression promoted resorption function. Mechanistically, NRP1 was identified...
In Escherichia coli, repression of the aceBAK operon is mediated by IclR protein. We used an in vitro oligonucleotide selection technique to determine consensus recognition sequence for MR. Mutational analysis confirmed contribution this vivo and identified -35 element promoter.
Osteoporosis is characterized by a decrease in bone mass and destruction of the microarchitecture, it commonly occurs postmenopausal women elderly. Overactivation osteoclasts caused inflammatory response or oxidative stress leads to osteoporosis. An increasing number studies have suggested that intracellular reactive oxygen species (ROS) are strongly associated with osteoclastogenesis. As novel angiotensin (Ang) II receptor blocker (ARB), azilsartan was reported be inhibition processes....
Recently, studies on microRNAs (miRNAs) and their targets related genes have provided new therapeutic opportunities for controlling intervertebral disc degeneration (IDD). We aimed to investigate the effects of miR-148a-3p overexpression IDD progression.This study used microRNA microarrays analyze key regulators IDD. Q-PCR was verify IL-1β-induced down-regulation expression both in nucleus pulposus (NP) tissues patients degenerated NP cells (NPCs) rats. Rat NPC micromass cultures ex vivo...
Background: Surufatinib is a potent small-molecule tyrosine kinase inhibitor and exhibited significant efficacy in the treatment of neuroendocrine tumors clinical trials. Objective: The absorption, metabolism excretion surufatinib were investigated rats human volunteers following single oral dose [14C] surufatinib. Methods: radioactivity was measured plasma, urine, feces bile by liquid scintillation counting, metabolites characterized chromatography coupled to mass spectrometry. Results:...
Abstract Objective: This study aimed to assess the feasibility as well clinical and radiological outcomes of posterior percutaneous pedicle screw fixation (PPSF) combined with anterior monosegmental column reconstruction in unstable thoracolumbar burst fractures. Methods: From January 2011 August 2017, thirty-five patients fractures were enrolled this study. The underwent PPSF surgery delayed utilizing titanium mesh cages. Clinical outcomes, parameters, treatment-related complications...
This study was aimed to construct the targeting AATF shRNA eukaryotic expression vector and establish stably transfected U937 cell lines. The sequence of mRNA obtained from GenBank. After excluding homology, three plasmid vectors coding 228 ∼ 249, 303 324 443 464 gene were synthesized. Two terminals carried BamHI HindIII restriction sites. selected nucleotides cloned into pSilencer 3.1-H1 neo respectively, resultant recombinant plasmids named as pSA-1, pSA-2, pSA-3. sequences identified by...
Primary signet-ring cell carcinoma of the bladder is a relatively rare tumor that progresses rapidly and has an extremely poor prognosis. We report case primary bladder. The patient underwent transurethral holmium laser lithotripsy for calculi in another hospital, was found intraoperatively. Bladder biopsy revealed infiltrating high-grade urothelial with adenoid differentiation invasion to muscular layer. Gastrointestinal endoscopy ruled out other possible sources tumor, admitted our...
Abstract Background： Macrophage migration inhibitory factor (MIF) is a pleiotropic pro-inflammatory mediator involved in various pathophysiological and inflammatory states.Accumulating line of evidence suggests role for MIF regulating bone metabolism therefore prime candidate therapeutic targeting. In this study, we showed that Chicago sky blue 6B (CSB6B), an unique allosteric inhibitor catalytic cytokine activity, suppresses RANKL-induced osteoclast promotes osteogenesis vitro via the...
We evaluated the effect of a novel modified OLIF technique (anteroinferior psoas approach, AIPA) for anterior decompression reconstruction in lumbar burst fractures, and compared clinical, radiological outcomes approach-related complications with mini-open, lateral transpsoas approach (LTPA).From March 2016 to November 2019, 68 patients fractures underwent one-stage monosegmental posterior/anterior surgery from L1-L4 segments. 35 included AIPA 33 LTPA group reconstruction. The functional...