A5032741035- Bone Metabolism and Diseases
- Bone health and treatments
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Spine and Intervertebral Disc Pathology
- NF-κB Signaling Pathways
- Osteoarthritis Treatment and Mechanisms
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Bone and Joint Diseases
- Bone health and osteoporosis research
- interferon and immune responses
- Kruppel-like factors research
- Cancer, Lipids, and Metabolism
- Connective tissue disorders research
- Cytokine Signaling Pathways and Interactions
- Musculoskeletal pain and rehabilitation
- Extracellular vesicles in disease
- Cell death mechanisms and regulation
- Inflammatory mediators and NSAID effects
- Metabolomics and Mass Spectrometry Studies
- Protease and Inhibitor Mechanisms
- Biomarkers in Disease Mechanisms
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
Sir Run Run Shaw Hospital
2017-2024
Zhejiang University
2017-2024
Blood Center of Zhejiang Province
2018-2024
Huzhou Central Hospital
2020
IHI Corporation (United States)
2018
Mitochondrial dysfunction and oxidative stress damage are hallmarks of osteoarthritis (OA). Mesenchymal stem cell (MSC)-derived exosomes important in intercellular mitochondria communication. However, the use MSC for regulating mitochondrial function OA has not been reported. This study aimed to explore therapeutic effect a three dimensional (3D) printed scaffold early therapeutics. Methods: We first examined mitochondria-related proteins normal human cartilage samples investigated whether...
Objectives Circular RNAs (circRNA) expression aberration has been identified in various human diseases. In this study, we investigated whether circRNAs could act as competing endogenous to regulate the pathological process of osteoarthritis (OA). Methods CircRNA deep sequencing was performed between OA and control cartilage tissues. The regulatory functional role CircSERPINE2 upregulation examined validated vitro vivo, downstream target explored. RNA pull down, a luciferase reporter assay,...
As a subclass of noncoding RNAs, circular RNAs (circRNAs) have been demonstrated to play critical role in regulating gene expression eukaryotes. Recent studies revealed the pivotal functions circRNAs cancer progression. However, little is known about circTADA2A, also named hsa_circ_0043278, osteosarcoma (OS).CircTADA2A was selected from previously reported circRNA microarray comparing OS cell lines and normal bone cells. QRT-PCR used detect circTADA2A tissue lines. Luciferase reporter, RNA...
The dysregulation of ROS production and osteoclastogenesis is involved in the progress osteoporosis. To identify novel effective targets to treat this disease, it important explore underlying mechanisms. In our study, we firstly tested effect Nrf2 activator RTA-408, a synthetic triterpenoid under clinical investigation for many diseases, on osteoclastogenesis. We found that could inhibit osteoclast differentiation bone resorption time- dose-dependent manner. Further, RTA-408 enhanced...
CircMYO10 is a circular RNA generated by back-splicing of gene MYO10 and upregulated in osteosarcoma cell lines, but its functional role still unknown. This study aimed to clarify the mechanism circMYO10 osteosarcoma.CircMYO10 expression 10 paired chondroma tissues was assessed quantitative reverse transcription polymerase chain reaction (PCR). The function circMYO10/miR-370-3p/RUVBL1 axis regarding two key characteristics: proliferation endothelial-mesenchymal transition (EMT)....
Inflammatory responses of nucleus pulposus (NP) can induce imbalanced anabolism and catabolism extracellular matrix, the cytosolic dsDNA accumulation STING-NF-κB pathway activation found in NP inflammation are considered as fairly important cause intervertebral disc (IVD) degeneration. Herein, we constructed a siSTING delivery hydrogel aldehyde hyaluronic acid (HA-CHO) poly(amidoamine) PAMAM/siRNA complex to intervene abnormal STING signal for IVD degeneration treatment, where formation...
Osteoarthritis (OA) is a common, age-related, and painful disease characterized by cartilage destruction, osteophyte formation, synovial hyperplasia. This study revealed that circPDE4D, circular RNA derived from human linear PDE4D, plays critical role in maintaining the extracellular cellular matrix (ECM) during OA progression. circPDE4D was significantly downregulated tissues stimulation with inflammatory cytokines. The knockdown of predominantly contributed to Aggrecan loss upregulation...
FBXO6, a component of the ubiquitin E3 ligases, has been shown to bind high mannose N-linked glycoproteins and act as ligase subunits. Most proteins in secretory pathway, such matrix metalloproteinases, are modified with N-glycans play important roles development osteoarthritis (OA). However, whether FBXO6 exerts regulatory effects on pathogenesis OA remains undefined.The expression was examined cartilage human multiple mouse models. The role degeneration analysed global FBXO6-/- mice,...
The endogenous metabolite itaconate has emerged as a regulator of macrophage function that limits inflammation. However, its effect on cell differentiation and osteoclast‐related diseases is unclear. Here, for the first time, we explored cell‐permeable derivative, 4‐octyl (OI) osteoclast in vitro vivo . Firstly, demonstrated concentration was lower estrogen‐deficient mice. OI released induced expression nuclear factor–erythroid 2–related factor 2 (Nrf2) bone marrow–derived macrophages during...
Osteoarthritis (OA) is a degenerative disease with series of metabolic changes accompanied by many altered enzymes. Here, we report that the down-regulated dimethylarginine dimethylaminohydrolase-1 (DDAH1) increased asymmetric (ADMA) in degenerated chondrocytes and OA samples. Global or chondrocyte-conditional knockout ADMA hydrolase DDAH1 accelerated development mice. induces degeneration senescence reduces extracellular matrix deposition, thereby accelerating progression. simultaneously...
Osteoporosis is one of the most common bone diseases in middle-aged and elderly populations worldwide. The development new drugs to treat disease a key focus research. Current treatments for osteoporosis are mainly directed at promoting osteoblasts inhibiting osteoclasts. However, there currently no ideal approach treatment. l-arginine semi-essential amino acid involved number cellular processes, including nitric production, protein biosynthesis, immune responses. We previously reported that...
ABSTRACT Osteoporosis develops because of impaired bone formation and/or excessive resorption. Although the pharmacological treatment osteoporosis has been extensively developed, alternative treatments are still needed. Here, we showed that oridonin (ORI), a diterpenoid isolated from Rabdosia rubescens, can suppress osteoclastogenesis and enhance osteogenesis. ORI inhibited receptor activator nuclear factor-κB (NF-κB) ligand (RANKL)-induced osteoclast resorption through inhibition p65...
BackgroundOsteoporosis is a common metabolic bone disease, which always leads to osteoporotic fractures. Biomarkers of mineral density (BMD) are helpful for prevention and early diagnosis osteoporosis. This study aims identify metabolomic biomarkers low BMD.MethodsWe included 701 participants who had BMD measures by dual-energy X-ray absorptiometry scans donated fasting plasma samples from three clinical centres as discovery set another 278 the fourth centre an independent replication set....
Mutations and altered expression of deubiquitinating enzymes (DUBs) profoundly influence tumor progression.Ubiquitin-specific protease 1 (USP1) is a well-characterized human DUB reportedly overexpressed in associated with maintaining the mesenchymal stem cell status osteosarcoma (OS); however, potential mechanisms USP1 OS remain poorly understood.In this study, we identified that directly interacts Transcriptional Co-Activator With PDZ-Binding Motif (TAZ) lines, mechanistic analysis...
High incidence of osteoporotic fractures emphasizes the necessity developing effective measures to promote osteogenesis. In our study, we investigated a possible role MAPK‐ERK signaling in TGF‐β‐mediated osteoblastic differentiation. Our results indicated that TGF‐β activated pathway and inhibited osteogenesis mesenchymal pluripotent cell line, C3H10T1/2, preosteoblastic MC3T3 cells. And downregulation using pharmacological inhibitor U0126 RNA interference rescued osteoblast differentiation...
Abstract Mechanical force is critical for the development and remodeling of bone. Here we report that mechanical regulates production metabolite asymmetric dimethylarginine (ADMA) via regulating hydrolytic enzyme dimethylaminohydrolase 1 ( Ddah1 ) expression in osteoblasts. The presence -394 4 N del/ins polymorphism higher serum ADMA concentration are negatively associated with bone mineral density. Global or osteoblast-specific deletion leads to increased level but reduced formation....
Ubiquitination is a reversible post-translational modification implicated in cell differentiation, homeostasis, and organ development. Several deubiquitinases (DUBs) decrease protein ubiquitination through the hydrolysis of ubiquitin linkages. However, role DUBs bone resorption formation still unclear. In this study, we identified DUB ubiquitin-specific protease 7 (USP7) as negative regulator osteoclast formation. USP7 combines with tumor necrosis factor receptor-associated 6 (TRAF6)...
Aims: Emerging evidence suggests that the pathogenesis of osteoporosis, characterized by impaired osteogenesis, is shifting from estrogen centric to oxidative stress. Our previous studies have shown zinc-finger transcription factor krüppel-like 5 (KLF5) plays a key role in degeneration nucleus pulposus and cartilage. However, its osteoporosis remains unknown. We aimed investigate effect mechanism KLF5 on osteogenesis under Results: First, was required for stimulated osteogenic...
Cartilaginous endplate (CEP) degeneration is an important cause for intervertebral disc (IVD) that leads to low-back pain. The identification of compounds may prevent CEP interest the prevention IVD degeneration.Catabolic protease expression in patients was first assessed. toxicity, function and underlying mechanism lycorine (LY) on CEP-derived chondrocytes were assessed vitro by flow cytometry analysis western blotting. concentration LY rat-tail disc-degeneration models also HPLC (High...
Abstract Bone metastasis is a severe complication of advanced breast cancer, resulting in osteolysis and increased mortality patients. Raddeanin A (RA), isolated from traditional Chinese herbs, an oleanane-type triterpenoid saponin with anticancer potential. In this study, we investigated the effects RA cancer-induced elucidated possible mechanisms involved process. We first verified that could suppress osteoclast formation bone resorption vitro. Next, confirmed suppressed...