A5023837512- Supramolecular Self-Assembly in Materials
- Chemical Synthesis and Analysis
- Surface Chemistry and Catalysis
- Protein Structure and Dynamics
- Polydiacetylene-based materials and applications
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Nanocluster Synthesis and Applications
- Bacteriophages and microbial interactions
- Alzheimer's disease research and treatments
- Biotin and Related Studies
- Cancer Research and Treatments
- Mass Spectrometry Techniques and Applications
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Molecular Sensors and Ion Detection
- Nanoparticle-Based Drug Delivery
- Force Microscopy Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Diatoms and Algae Research
- Immunotherapy and Immune Responses
Fujian Normal University
2021-2025
Guangzhou Institutes of Biomedicine and Health
2021-2025
University of Hong Kong
2025
Ministry of Education
2024
Chinese Academy of Sciences
2018-2021
National Center for Nanoscience and Technology
2017-2021
University of Chinese Academy of Sciences
2018-2021
Tsinghua University
2017-2020
Center for Excellence in Brain Science and Intelligence Technology
2019
Center for NanoScience
2019
T cell infiltration into tumors is essential for successful immunotherapy against solid tumors. Herein, we found that the expression of hyaluronic acid synthases (HAS) was negatively correlated with patient survival in multiple types including gastric cancer. HA impeded vitro anti-tumor activities anti-mesothelin (MSLN) chimeric antigen receptor cells (CAR-T cells) cancer by restricting CAR-T mobility . We then constructed a secreted form human hyaluronidase PH20 (termed sPH20-IgG2)...
We have determined the interaction strengths of common naturally occurring amino acids using a complete binding affinity matrix 20 × pairs homo-octapeptides consisting between stationary and mobile states. used bead-based fluorescence assay for these measurements. The results provide basis analyzing specificity, polymorphisms, selectivity inter-amino-acid interactions. Comparative analyses energies, i.e., free energies association (ΔGA), reveal contributions assignable to both...
Abstract Peptidoglycan, an essential component within the cell walls of virtually all bacteria, is composed glycan strands linked by stem peptides that contain D‐amino acids. The peptidoglycan biosynthesis machinery exhibits high tolerance to various acid derivatives. derivatives with different functionalities can thus be specifically incorporated into and label but not host mammalian cells. This metabolic labeling strategy highly selective, biocompatible, broadly applicable, which has been...
Amino acid chirality has been recognized as an important driving force in constructing peptide architectures, via interactions such chirality-induced stereochemical effect. The introduction of site-specific chiral conversion l- and d-amino acids sequences could enable the pursuit effects assembly. In this work, we characterized assemblies heptapeptides with various side chain moieties their variants using STM. Specifically, two pairs amino acids, Gln (Q) Asn (N), Glu (E) Asp (D), having one...
The characteristics of peptide conformations in both solution and surface-bound states, using poly-glycine as a model structure, are analyzed by molecular dynamics (MD) simulations. clustering analysis revealed significant linearization effect on the result adsorption to surface, accompanied varied kinetics energetics. Depending inter-peptide interaction characteristics, distinctively different surface-mediated oligomerization modalities, such antiparallel conformations, can be identified MD...
A set of the homogeneous and heterogeneous octapeptides have been studied for composition sequence effects on surface-mediated assemblies at liquid–solid interfaces by using scanning tunneling microscopy. The observed assembly structures graphite surface reveal that all these peptides can form lamella characteristics. By analyzing distributions width intralamella peptide strand length, it is suggested consisting tryptophan glycine in present experiments do not a significant impact assembling...
Amino acid chirality has been envisioned as an important strategy to regulate structure and function of peptide self-assembled architectures. However, the molecular mechanism effects in assemblies remains largely elusive. Here, assembly structures l-peptide polyphenylalanine F10 (FFFFFFFFFF) block heterochiral F5f5 (FFFFFfffff) composed two FFFFF repeat blocks with opposite were characterized at single-molecule level by using scanning tunneling microscopy. Each formed distinctively different...
We demonstrate in this contribution the evidence that significant cooperative binding effect can be identified for amino acid sites are determinant to characteristics peptide–peptide interactions. The analysis of tryptophan‐scanning mutagenesis 14‐mer peptide consisting only glycine provides a mapping position‐dependent contributions energy. pronounced tryptophan‐associated interactions originated from indole moieties with main chains glycines containing N–H and CO moieties. Specifically,...
Abstract The abnormal accumulation of beta‐amyloids (Aβ) in brain is considered as a key initiating cause for Alzheimer's disease (AD) due to their richness plaques and self‐aggregate propensity. In recent studies, N‐terminally extended Aβ peptides (NTE‐Aβ) with the N‐terminus originating prior canonical β‐secretase cleavage site were found humans suggested have possible relevance AD. However, effects on amyloidegenic structure aggregation propensity not been fully elucidated. Herein, we...
We have examined in this contribution the composition dependence of binding characteristics peptide-peptide interactions between an oligopeptide octa-glycine and a series tryptophan-containing octapeptides. The energy associated with tryptophan-glycine manifests pronounced stepwise as number tryptophan increases from 0 to 8 octapeptides consisting only glycine can be attributed mono-, di-, tri-valent interactions. At same time, weak fluctuations were observed 2 7. Such distinctive...
A metabolically-driven probe targets Gram-positive bacteria, offering imaging and inactivation via streptavidin-modified reagents, showcasing innovative metabolic incorporation.
Enantiomeric peptides with opposing molecular chirality self-assembled into nanostructures the same handedness.
An understanding of protein conformational ensembles is essential for revealing the underlying mechanisms interpeptide recognition and association. However, experimentally resolving multiple simultaneously existing substates remains challenging. Here, we report use scanning tunneling microscopy (STM) to analyze substate β sheet peptides with a submolecular resolution (in-plane <2.6 Å). We observed more than 10 (with free energy fluctuations between several kBTs) in peptide homoassemblies...
Surface modification can impart nanostructures new interface properties. In this review, we summarize the representative surface methods of nanoparticles with peptides and proteins. The biomolecules be conjugated by noncovalent covalent coupling. Both these approaches have strengths limitations. Physical adsorption is most direct simplest method but proteins adsorbed on solid always loss their native structures biological activity behavior still difficult to regulate precisely. A popular...
In this review, we try to reflect the recent progress on assembly structures, aggregation behaviors and cytotoxicity of beta amyloid (Aβ) peptides, which are closely related pathogenesis Alzheimer's disease (AD).Based these studies, regulation mechanism various nanobiointerfaces in peptide structures is reviewed discussed, especially interaction patterns mechanisms between Aβ peptides nanomaterials molecule level.These progresses could help deepen insights complex interactions modulators.To...