A5087866098- Sperm and Testicular Function
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Virus-based gene therapy research
- Kruppel-like factors research
- Reproductive System and Pregnancy
- Lipid metabolism and biosynthesis
- Allergic Rhinitis and Sensitization
- Pregnancy and preeclampsia studies
- Exercise and Physiological Responses
- Dermatology and Skin Diseases
- Viral gastroenteritis research and epidemiology
- Hormonal and reproductive studies
- Reproductive biology and impacts on aquatic species
Juntendo University
2024
Osaka University
2016-2020
Institute for Animal Reproduction
1992
Chiba University
1992
Abstract More than 1000 genes are predicted to be predominantly expressed in mouse testis, yet many of them remain unstudied terms their roles spermatogenesis and sperm function essentiality male reproduction. Since individually indispensable factors can provide important implications for the diagnosis genetically related idiopathic infertility may serve as candidate targets development nonhormonal contraceptives, our laboratories continuously analyze functions testis-enriched vivo by...
Local control of sperm maturation Newly produced spermatozoa within the testis do not have fertilizing ability but become fully functional when they mature in epididymis. The development epididymis itself is dependent on testicular factors arriving via luminal flow. Improper signaling between and hypothesized to result male infertility. Kiyozumi et al. identified NELL2 as a protein that binds its receptor, ROS1, surface induces epididymal differentiation (see Perspective by Lord Oatley). In...
Abstract Spermatozoa are produced in the testis but gain their fertilizing ability during epididymal migration. This necessary step sperm maturation includes posttranslational modification of membrane proteins that protein processing by proteases. However, molecular mechanism underpinning this remains unknown. In study, we focused on transmembrane serine protease 12 (Tmprss12). Based multi-tissue expression analysis PCR, Tmprss12 was specifically expressed testis, and its started day 10...
Atopic dermatitis (AD), a chronic inflammatory skin disease, manifests as an intractable itch. Psychological stress has been suggested to play role in the onset and worsening of AD symptoms. However, pathophysiological relationships between psychological stressors cutaneous manifestations remain unclear. To elucidate mechanisms underlying stress-related exacerbation itch, we investigated effects water stress, restraint repeated social defeat on itch-related scratching behaviour, mechanical...
Several hundred fertilized pig eggs were prepared by an in vitro fertilization (IVF) technique which follicular phase ovarian matured to metaphase II before incubation with capacitated epididymal spermatozoa for 12 h at 39 degrees C. Parthenogenetic also stimulation of the mature electric pulse. The zonae solubilized 0.2% pronase/phosphate-buffered saline (PBS) or lactic acid/PBS. time taken solubilization was 30-40% shorter than unfertilized eggs, indicating that zona hardening induced...
The X-linked Plac1 gene is maternally expressed in trophoblast cells during placentation, and its disruption causes placental hyperplasia intrauterine growth restriction. In contrast, also reported to be one of the upregulated genes hyperplastic placenta generated by nuclear transfer. However, effect overexpressed on formation function remained unaddressed. We complemented knockout dysfunction lentiviral vector-mediated, placenta-specific transgene expression. Whereas fetal development...
Preeclampsia is a systemic disease caused by abnormal placentation that affects both mother and fetus. It was reported Laeverin (LVRN, also known as Aminopeptidase Q) up-regulated in the placenta of preeclamptic patients. However, physiological pathological functions LVRN remained to be unknown. Here we characterized Lvrn function during mice. RT-PCR showed expressed fetus embryogenesis, several adult tissues. When overexpressed placenta-specific manner using lentiviral vectors, did not see...