A5074393517- Plant tissue culture and regeneration
- Horticultural and Viticultural Research
- Plant Reproductive Biology
- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Seed Germination and Physiology
- Plant Physiology and Cultivation Studies
- Transgenic Plants and Applications
- Plant and soil sciences
- Dye analysis and toxicity
- Growth and nutrition in plants
- Plant Molecular Biology Research
- Fermentation and Sensory Analysis
- Polysaccharides and Plant Cell Walls
- Banana Cultivation and Research
- Plant nutrient uptake and metabolism
- Fish Biology and Ecology Studies
- Powdery Mildew Fungal Diseases
- Plant Stress Responses and Tolerance
- Garlic and Onion Studies
- Aquaculture disease management and microbiota
- Light effects on plants
- Botanical Research and Applications
- Heavy metals in environment
- Genetically Modified Organisms Research
Centro de Investigación en Alimentación y Desarrollo
2008-2023
Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food
2021
Establishment of an efficient explants surface disinfection protocol is essential for in vitro cell and tissue culture as well germplasm conservation, such the case Grapevine (Vitis spp.) culture. In this research, different procedures regeneration field-grown grapevine cv. 'Flame seedless' axillary buds were evaluated. The disinfected using either NaOCl or allyl, benzyl, phenyl 2-phenylethyl isothiocyanates. Two media shooting four rooting tested. Shoot root development per registered. best...
Wheat cultivation makes an important contribution to human nutrition. Trehalose synthesis plays a role in the tolerance drought stress. A bifunctional TPS-TPP enzyme gene from yeast was used obtain transgenic wheat plants increase trehalose synthesis. Mature embryos were transformed using pGreen rd29A::TPS1-TPS2 or 35S::TPS1-TPS2 constructs. The transgene presence mature leaves of T3 confirmed by sequencing PCR fragment inserted transgene. Transgenic and NT submitted stress for eight days....
This study proposes the use of volatile sulfur compounds derived from garlic (Allium sativum) extracts applied via gas for control Botrytis cinerea, which causes post-harvest disease in table grapes. The effects emitted by extract and sodium metabisulfite on conidia germination B. cinerea were evaluated vitro to assess their effectiveness at controlling grey mold grapes stored different temperatures.Diallyl disulfide, diallyl trisulfide allicin identified quantified a using reversed-phase...
Cryopreservation is used for the long-term conservation of plant genetic resources. This technique very often induces lethal injury or tissue damage. In this study, we measured indicators viability and cell damage following cryopreservation vitrification-cryopreservation in Vitis vinifera L. axillary buds cv. "Flame seedless" stored liquid nitrogen (LN) for: three seconds, one hour, day, week month; after LN thawed at 38 °C minutes. The enzymatic activity catalase (CAT) superoxide dismutase...
El efecto germicida de la irradiación UV-C se ha empleado diferentes alimentos como un método desinfección superficial a temperatura ambiente que no deja residuos en el producto, por lo considera una buena alternativa para conservación alimentos. Su utilización dosis bajas tenido éxito desinfecciónde frutas y hortalizas. Algunos efectos benéficos atribuidos son: inducción mecanismos defensa (síntesis fitoalexinas), los cuales relacionan positivamente con resistencia patógenos reducción...
Plant tissues must be preserved in their collection state, especially for genome-wide expression profile studies. Lyophilization is a feasible, affordable tool when fresh cannot shipped at ultralow temperatures from origin to the place of analysis. In this study, total RNA quality dormant grapevine buds (Vitis vinifera L. cv. 'Flame Seedless') freeze-dried samples stored room temperature conditions was evaluated and compared that cryopreserved (-80°C) buds. Good yield were obtained 0, 3 6...
Grapes are a major source of phenolic compounds. Phenolic compounds play an important role in the quality grapes and wines, antioxidant activity has been positively correlated with their composition. This work was focused on identification fruit extracts ‘Norton’, also known as ‘Cynthiana’ (Vitis aestivalis). Identification quantification were performed using high performance liquid chromatography-mass spectrometry (HPLC-MS TOF) electrospray ionization interface negative mode ion. Detection...
Mexico is a large producer of table grape (Vitis vinifera L.) and therefore it important to develop protocols store the varieties germplasm.The objective present work was design protocol for cryopreservation by vitrification zygotic embryos V. cv."Red Globe" evaluate possible epigenetics changes.The plant solution 2 (PVS2) utilized before utilization liquid nitrogen (LN).The effect this on embryo viability tested triphenyl-tetrazolium chloride solution, as well in vitro development into...
La vid es uno de los principales cultivos del mundo y el estado Sonora mayor productor México. Este cultivo está en constante peligro por diversos factores bióticos abióticos, ahí la importancia su conservación. crioconservación idónea para este cultivo, pero puede provocar alteraciones fisiológicas, moleculares bioquímicas, afectando viabilidad regeneración. Por lo anterior, trabajo se planteó analizar efecto las etapas protocolo sobre regeneración embriones cigóticos vid. tejido (V)...
La vid es un cultivo importante en México y Sonora líder producción. Sin embargo, no existe método adecuado para la conservación del germoplasma. crioconservación (almacenamiento nitrógeno líquido (NL)) una alternativa a largo plazo e involucra técnicas de crioprotección (vitrificación encapsulación-deshidratación) evitar el daño celular durante congelamiento. En este trabajo se evaluó pérdida iónica yemas sometidas mediante vitrificación encapsulación deshidratación. Para las fueron PVS2...