A5070405832- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Botany, Ecology, and Taxonomy Studies
- Botanical Research and Applications
- Plant and soil sciences
- Plant Diversity and Evolution
- Plant nutrient uptake and metabolism
- Seed Germination and Physiology
- Plant Genetic and Mutation Studies
- Fungal and yeast genetics research
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Genetically Modified Organisms Research
- Photosynthetic Processes and Mechanisms
- Tardigrade Biology and Ecology
- RNA Research and Splicing
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Pineapple and bromelain studies
- Plant Pathogens and Fungal Diseases
- Microtubule and mitosis dynamics
- Plant Pathogens and Resistance
- Botany and Geology in Latin America and Caribbean
- Biocrusts and Microbial Ecology
- Coffee research and impacts
Chapingo Autonomous University
2010-2024
Universidad Autónoma del Estado de Morelos
2004
Universidad Nacional Autónoma de México
1999-2004
Abstract In Arabidopsis (Arabidopsis thaliana), trehalose is present at almost undetectable levels, excluding its role as an osmoprotectant. Here, we report that overexpression of AtTPS1 in using the 35S promoter led to a small increase and trehalose-6-P levels. spite this, transgenic plants displayed dehydration tolerance phenotype without any visible morphological alterations, except for delayed flowering. Moreover, seedlings overexpressing exhibited glucose (Glc)- abscisic acid...
Abstract The accumulation of the disaccharide trehalose in anhydrobiotic organisms allows them to survive severe environmental stress. A plant cDNA,SlTPS1, encoding a 109-kD protein, was isolated from resurrection Selaginella lepidophylla, which accumulates high levels trehalose. Protein-sequence comparison showed that SlTPS1 shares similarity trehalose-6-phosphate synthase genes prokaryotes and eukaryotes. SlTPS1mRNA constitutively expressed S. lepidophylla. DNA gel-blot analysis indicated...
Plants, such as Arabidopsis thaliana and Selaginella lepidophylla, contain genes homologous with the trehalose-6-phosphate synthase (TPS) of bacteria fungi. Most plants do not accumulate trehalose desert resurrection plant S. being a notable exception. Overexpression in Saccharomyces cerevisiae tps1 mutant results very low TPS-catalytic activity accumulation. We show that truncation plant-specific N-terminal extension A. AtTPS1 lepidophylla SlTPS1 homologues 10-40-fold higher TPS 20-40-fold...
Wheat cultivation makes an important contribution to human nutrition. Trehalose synthesis plays a role in the tolerance drought stress. A bifunctional TPS-TPP enzyme gene from yeast was used obtain transgenic wheat plants increase trehalose synthesis. Mature embryos were transformed using pGreen rd29A::TPS1-TPS2 or 35S::TPS1-TPS2 constructs. The transgene presence mature leaves of T3 confirmed by sequencing PCR fragment inserted transgene. Transgenic and NT submitted stress for eight days....
Garlic yield and quality have decreased due to white rot disease caused by Sclerotium cepivorum Berk. A transformation protocol introduce tobacco chitinase glucanase genes into garlic embryogenic calli using Agrobacterium tumefaciens has been established. LBA4404 strain having pC2301CHGLU plasmid with TaCh, glu, gus nptII (coding for chitinase, glucanase, β-glucuronidase neomycin phosphotransferase, respectively) was used. 30 putative transgenic clones were obtained from inoculated after six...
Opuntia ist eine Pflanzengattung, die als Kaktusfeige (nopales in Mexiko) bekannt und mit etwa 200 Arten taxonomisch komplex ist. Die vorliegende Studie konzentrierte sich auf Charakterisierung der Morphologie, Mikromorphologie sowie Erstellung einer ersten phylogenetischen Hypothese für chiangiana, 2009 beschriebenen Art. Drei Individuen wurden charakterisiert Santa María Mixistlán (Gemeinde de la Reforma, Oaxaca, gesammelt. Es wurde vollständige taxonomische Dokumentation erstellt, Bilder...
Tomato is considered moderately sensitive to salinity, which detracts from the quality and yield of its fruit; therefore, wild populations have been used as a genetic resource. The aim this research was identify lines derived tomato with tolerance salinity during germination seedling stages. During germination, 52 2 commercial hybrids were subjected 150 mM 0 NaCl evaluated. test carried out for 20 days in chamber constant darkness, temperature 25±2 °C relative humidity 80±4%. At stage, 22...
A detailed morphological and molecular study of the four Opuntia species belonging to ser. Streptacanthae is carried out. No published phylogenetic includes these species. Our data support recognition taxa as separated but related by characteristic having a glabrous epidermis, yellow flowers, red sweet fruits, in present it recovered forming monophyletic group within s.str.
Tomato is considered moderately sensitive to salinity, which detracts from the quality and yield of its fruit; therefore, wild populations have been used as a genetic resource. The aim this research was identify lines derived tomato with tolerance salinity during germination seedling stages. During germination, 52 2 commercial hybrids (Imperial®, Reserva®) were subjected treatment 150 mM 0 NaCl evaluated. test carried out for 20 days in chamber constant darkness, temperature 25 ± °C relative...
Calla lily (Zantedeschia spp.) is an herbaceous flowering plant that belongs to the Araceae family, with worldwide distribution. Native swampy or mountainous regions of South Africa, it valued as ornamental due its extraordinary spathe and decorative leaves. However, production has decreased presence diseases. In vitro culture cells tissues been successfully applied precisely diagnose control diseases produce disease-free plants. This study analyzes information from in techniques this crop,...
La variedad de café Colombia (Coffea arabica L.) tiene alta resistencia a la roya y buenas características producción calidad taza. Diversos protocolos para propagación mediante embriogénesis somática han sido desarrollados; sin embargo, tasa maduración, germinación conversión es baja. El objetivo presente investigación fue determinar el efecto reguladores del crecimiento agentes osmóticos en maduración embriones somáticos evaluar, condiciones ex vitro, las fisiológicas plantas regeneradas....
Genotypes of the chrysanthemum cultivars Harman and Indianapolis were transformed using Agrobacterium tumefaciens strain LBA4404, which carries binary plasmid pBIN19::Rd29A::ScTPS1TPS2::nos pBIN19::35S::ScTPS1TPS2::nos, respectively. ISSR markers used to discriminate two from each other, independent transgenic plants generated in cultivar. Firstly, nptII gene Tnos sequence genotypes was confirmed by PCR. For genotyping, 10 profile produced 131 DNA bands. The percentage polymorphism ranged 20...
Opuntia is a genus of plants known as prickly pear cactus (nopales), with about 200 species, and taxonomically complicated. The present study was focused on characterizing micromorphology providing phylogenetic hypothesis to place setocarpa (described in 2017) relation other related species. Three individuals were characterized collected Neverías the Sierra Cacoma municipality Autlán de Navarro Jalisco, Mexico. spines, epidermis stoma scanning electron microscopy. performed maximum...