A5075821680- Neuroinflammation and Neurodegeneration Mechanisms
- Leech Biology and Applications
- Extracellular vesicles in disease
- Neuropeptides and Animal Physiology
- Healthcare and Venom Research
- Immune cells in cancer
- Neurobiology and Insect Physiology Research
- Reproductive Biology and Fertility
- Antimicrobial Peptides and Activities
- MicroRNA in disease regulation
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Pain Mechanisms and Treatments
- Computational Drug Discovery Methods
- Parasites and Host Interactions
- Invertebrate Immune Response Mechanisms
- Neuroendocrine regulation and behavior
- Plant-based Medicinal Research
- Autophagy in Disease and Therapy
- Spinal Cord Injury Research
- Aquatic life and conservation
- Cardiac Imaging and Diagnostics
- Aquaculture Nutrition and Growth
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2013-2025
Inserm
2014-2025
Université de Lille
2008-2025
Institut de Physique et Chimie des Matériaux de Strasbourg
2024
Centre National de la Recherche Scientifique
2000-2021
CEA Paris-Saclay
2021
Institut de Biologie Intégrative de la Cellule
2019-2021
Université Paris-Saclay
2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021
Laboratoire de Biologie et Modélisation de la Cellule
2019
Vertebrate oocytes arrest in the second metaphase of meiosis (metaphase II [MII]) by an activity called cytostatic factor (CSF), with aligned chromosomes and stable spindles. Segregation occurs after fertilization. The Mos/…/MAPK (mitogen-activated protein kinases) pathway mediates this MII arrest. Using a two-hybrid screen, we identified new MAPK partner from mouse oocyte cDNA library. This is unstable during first meiotic division accumulates only MII, where it localizes to spindle. It...
Two novel antimicrobial peptides named theromacin and theromyzin were isolated characterized from the coelomic liquid of leech Theromyzon tessulatum. Theromacin is a 75-amino acid cationic peptide containing 10 cysteine residues arranged in disulfide array showing no similarities with other known peptides. Theromyzin an 86-amino linear constitutes first anionic observed invertebrates. Both exhibit activity directed against Gram-positive bacteria. cDNAs code precursor molecules putative...
Abstract The functional preservation of the central nervous system (CNS) is based on neuronal plasticity and survival. In this context, neuroinflammatory state plays a key role involves microglial cells, CNS-resident macrophages. order to better understand contribution neuroprotection, microglia-derived extracellular vesicles (EVs) were isolated molecularly characterized be then studied in neurite outgrowth assays. EVs, mainly composed exosomes microparticles, are an important cell-to-cell...
In Mammals, microglial cells are considered as the resident immune in central nervous system (CNS). Many studies demonstrated that, after injury, these activated and recruited at lesion site. Leech microglia present a similar pattern of activation migration upon experimental CNS. This is associated with release large amount extracellular vesicles (EVs). We collected EVs released by primary culture compared two different protocols isolation: one differential ultracentrifugation (UC) using an...
For the success of fertilization, spindles vertebrate oocytes must remain stable and correctly organized during arrest in metaphase II meiosis. Using a two-hybrid screen with MAPK as bait, we have recently identified MISS (MAPK interacting spindle stabilizing) which controls mouse oocyte stability. same screen, identify another partner, DOC1R (Deleted oral cancer one related), murine homologue potential human tumor suppressor gene. We characterize meiosis resumption. is regulated by...
The Ionized calcium-Binding Adapter molecule 1 (Iba1), also known as Allograft Inflammatory Factor (AIF-1), is a 17 kDa cytokine-inducible protein, produced by activated macrophages during chronic transplant rejection and inflammatory reactions in Vertebrates. In mammalian central nervous system (CNS), Iba1 sensitive marker associated with macrophages/microglia upregulated following neuronal death or brain lesions. medicinal leech Hirudo medicinalis able to regenerate its CNS after injury,...
<title>Abstract</title> Alzheimer’s disease (AD) is the most prominent form of dementia worldwide. It characterized by tau lesions that spread throughout brain in a spatio-temporal manner. This has led to prion-like propagation hypothesis implicating transfer pathological seeds from cell-to-cell. Human extracellular vesicles isolated brain-derived fluid (BD-EVs) AD patients contain contribute this pathology spreading. Knowing rich diversity EVs, isolation functional EVs sub-population...
Based on proteomic analyses we investigated the differences of released molecules in conditioned media (CM) from spinal cord central lesion and adjacent rostral caudal segments at 3, 7, 10 days after injury (SCI), order to specify molecular environment within greater extent tissue damage. Proteins found CM were analyzed by shot-gun MS using nanoLC coupled an orbitrap. The results showed some specific proteins each site 3days. Among segments, are related chemokines, cytokines or neurogenesis...
In healthy or pathological brains, the neuroinflammatory state is supported by a strong communication involving microglia and neurons. Recent studies indicate that extracellular vesicles (EVs), including exosomes microvesicles, play key role in physiological interactions between cells allowing central nervous system (CNS) development and/or integrity. The present report used medicinal leech CNS to investigate microglia/neuron crosstalk from ex vivo approaches as well primary cultures....
Combining proteomics and systems biology approaches, we demonstrate that neonatal microglial cells derived from two different CNS locations, cortex spinal cord, cultured in vitro displayed phenotypes upon physiological or pathological conditions. These also exhibited greater variability terms of cellular small extracellular vesicles (sEVs) protein content levels. Bioinformatic data analysis showed cortical microglia exerted anti-inflammatory neurogenesis/tumorigenesis properties, while the...
At the present time, there is little information on mechanisms of innate immunity in invertebrate groups other than insects, especially annelids. In study, we have performed a transcriptomic study immune response leech Theromyzon tessulatum after bacterial challenge, by combination differential display RT (reverse transcriptase)–PCR and cDNA microarrays. The results show relevant modulations concerning several known unknown genes. Indeed, threonine deaminase, malate dehydrogenase, cystatin...
Abstract In contrast to mammals, the medicinal leech Hirudo medicinalis can completely repair its central nervous system (CNS) after injury. This invertebrate model offers unique opportunities study molecular and cellular basis of CNS processes. When is injured, microglial cells migrate accumulate at site lesion, a phenomenon known be essential for usual sprouting injured axons. present study, we demonstrate that new molecule, designated Hm IL‐16, having functional homologies with human...
Abstract In the present paper we develop a new non-cell based (cell-free) therapeutic approach applied to BV2 microglial cells and spinal cord derived primary microglia (PM) using conditioned media from rat bone marrow stromal (BMSCs-CM). First collected (CM) either naive or injured tissue (SCI-CM, inflammatory stimulation agent) (BMSCs-CM, immunomodulation agent). They were both subsequently checked for presence of chemokines growth, neurotrophic neural migration factors proteomics...
Abstract Background In invertebrates, the medicinal leech is considered to be an interesting and appropriate model study neuroimmune mechanisms. Indeed, this non-vertebrate animal can restore normal function of its central nervous system (CNS) after injury. Microglia accumulation at damage site has been shown required for axon sprouting efficient regeneration. We characterized Hm C1q as a novel chemotactic factor microglial cell recruitment. mammals, C1q-binding protein (C1qBP alias gC1qR),...
Background:The medicinal leech is considered as a complementary and appropriate model to study immune functions in the central nervous system (CNS). In context which an injured leech's CNS can naturally restore normal synaptic connections, accumulation of microglia (immune cells that are exclusively resident leeches) has been shown be essential at lesion engage axonal sprouting. HmC1q (Hm for Hirudo medicinalis) possesses chemotactic properties important microglial cell recruitment by...
Neuronal activity is closely influenced by glia, especially microglia which are the resident immune cells in central nervous system (CNS). Microglia medicinal leech only able to migrate injury site within 24 hours post-lesion. The microglia-neuron interactions constitute an important mechanism as there neither astrocyte nor oligodendrocyte CNS. Given that axonal sprouting impaired when recruitment inhibited, crosstalk between and neurons plays a crucial role neuroprotection. present results...