A5069849721- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Extracellular vesicles in disease
- RNA Research and Splicing
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Single-cell and spatial transcriptomics
- Advanced Proteomics Techniques and Applications
- Cancer Research and Treatments
- Virus-based gene therapy research
- Metabolism and Genetic Disorders
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Cell Adhesion Molecules Research
- S100 Proteins and Annexins
- NF-κB Signaling Pathways
- Machine Learning in Bioinformatics
- Signaling Pathways in Disease
- Reproductive System and Pregnancy
- 3D Printing in Biomedical Research
- Protein Degradation and Inhibitors
- Antimicrobial Peptides and Activities
- CAR-T cell therapy research
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2017-2024
Inserm
2017-2024
Université de Lille
2018-2024
Centre Hospitalier Universitaire de Lille
2022-2024
European Graduate School of Neuroscience
2019
Abstract Background Cancer heterogeneity is a main obstacle for the development of effective therapies, as its replication in vitro preclinical models challenging. Around 96% developed drugs are estimated to fail from discovery clinical trial phase probably because unsuitability and unreliability current (Front Pharmacol 9:6, 2018; Nat Rev 8: 147–56, 2008) replicating overall biology tumors, instance tumor microenvironment. Breast cancer most frequent among women causing greatest number...
3D tumoroids have revolutionized in vitro/ex vivo cancer biology by recapitulating the complex diversity of tumors. While provide new insights into development and treatment response, several limitations remain. As tumor microenvironment, especially immune system, strongly influences development, absence cells may lead to inappropriate conclusions. Macrophages, key players progression, are particularly challenging integrate tumoroids. In this study, we established three optimized...
Tissue spatially-resolved proteomics was performed on 3 brain regions, leading to the characterization of 123 reference proteins. Moreover, 8 alternative proteins from open reading frames (AltORF) were identified. Some display specific post-translational modification profiles or truncation linked regions and their functions. Systems biology analysis proteome identified in each region allowed associate sub-networks with functional physiology region. Back correlation by at a given tissue...
Abstract The functional preservation of the central nervous system (CNS) is based on neuronal plasticity and survival. In this context, neuroinflammatory state plays a key role involves microglial cells, CNS-resident macrophages. order to better understand contribution neuroprotection, microglia-derived extracellular vesicles (EVs) were isolated molecularly characterized be then studied in neurite outgrowth assays. EVs, mainly composed exosomes microparticles, are an important cell-to-cell...
In Mammals, microglial cells are considered as the resident immune in central nervous system (CNS). Many studies demonstrated that, after injury, these activated and recruited at lesion site. Leech microglia present a similar pattern of activation migration upon experimental CNS. This is associated with release large amount extracellular vesicles (EVs). We collected EVs released by primary culture compared two different protocols isolation: one differential ultracentrifugation (UC) using an...
Identifying new lipid markers linked to traumatic brain injury (TBI) is of major importance in characterizing their central role the regeneration process and inflammatory response such an model. In present study, advanced lipidomics analysis using high spectral resolution matrix-assisted laser desorption/ionization-mass spectrometry imaging was performed on different regions experimental rat model moderate controlled cortical impact (CCI) while considering time points (1 day, 3 days, 7 10...
In healthy or pathological brains, the neuroinflammatory state is supported by a strong communication involving microglia and neurons. Recent studies indicate that extracellular vesicles (EVs), including exosomes microvesicles, play key role in physiological interactions between cells allowing central nervous system (CNS) development and/or integrity. The present report used medicinal leech CNS to investigate microglia/neuron crosstalk from ex vivo approaches as well primary cultures....
Traumatic brain injury (TBI) represents a major health concerns with no clinically-approved FDA drug available for therapeutic intervention. Several genomics and neuroproteomics studies have been employed to decipher the underlying pathological mechanisms involved that can serve as potential neurotherapeutic targets unveil possible relation of TBI other secondary neurological disorders. In this work, we present novel high throughput systems biology approach using spatially resolved...
Combining proteomics and systems biology approaches, we demonstrate that neonatal microglial cells derived from two different CNS locations, cortex spinal cord, cultured in vitro displayed phenotypes upon physiological or pathological conditions. These also exhibited greater variability terms of cellular small extracellular vesicles (sEVs) protein content levels. Bioinformatic data analysis showed cortical microglia exerted anti-inflammatory neurogenesis/tumorigenesis properties, while the...
We report, for the first time, detection and specific localization of long-chain acylcarnitines (LC ACs) along lesion margins in an experimental model spinal cord injury (SCI) using 3D mass spectrometry imaging (MSI). Acylcarnitines palmitoylcarnitine (AC(16:0)), palmitoleoylcarnitine (AC(16:1)), elaidic carnitine (AC(18:1)) tetradecanoylcarnitine (AC(14:1)) were detected as early 3 days post injury, present 7 10 after SCI induced by balloon compression technique rat. MSI revealed...
Abstract Chimeric antigen receptor (CAR)-T-cell therapy has revolutionized cellular immunotherapy, demonstrating remarkable efficacy in hematological cancers. However, its application solid tumors faces significant challenges, including limited T-cell infiltration and tumor-induced immunosuppression. Given the prominent role of macrophages tumor microenvironment, their phenotypic plasticity inherent antitumor properties, such as phagocytosis, offer a promising avenue for therapeutic...
Neuronal activity is closely influenced by glia, especially microglia which are the resident immune cells in central nervous system (CNS). Microglia medicinal leech only able to migrate injury site within 24 hours post-lesion. The microglia-neuron interactions constitute an important mechanism as there neither astrocyte nor oligodendrocyte CNS. Given that axonal sprouting impaired when recruitment inhibited, crosstalk between and neurons plays a crucial role neuroprotection. present results...
Using multi-omics analyses including RNAseq, RT-PCR, RACE-PCR, and shotgun proteomic with enrichment strategies, we demonstrated that newborn rat astrocytes produce neural immunoglobulin constant variable heavy chains as well light chains. However, their edification is different from the ones found in B cells they resemble aberrant immunoglobulins observed several cancers. Moreover, complete enzymatic V(D)J recombination complex has also been identified astrocytes. In addition, chain present...
During tumorigenesis, macrophages are recruited by tumors and orientated towards a pro-tumoral phenotype. One of the main anti-tumoral immunotherapy consists their re-polarization in an We have demonstrated that inhibition proprotein convertase 1/3 combined with TLR4 activation is promising strategy. These display pro-inflammatory phenotypes. A hallmark stronger NFKB pathway. believe this can be explained modification expression at cell surface or MYD88 cleavage since it exhibits potential...
The dogma "One gene, one protein" is clearly obsolete since cells use alternative splicing and generate multiple transcripts which are translated into protein isoforms, but also translation initiation sites (TISs) termination on a given transcript. Alternative open reading frames for individual give proteins originate from the 5'- 3'-UTR mRNA regions, frameshifts of ORFs or non-coding RNAs. Longtime considered as non-coding, recent in-silico prediction methods enriched databases allowing...
The neuroinflammatory state of the central nervous system (CNS) plays a key role in physiological and pathological conditions. Microglia, resident immune cells brain, sometimes infiltrating bone marrow-derived macrophages (BMDMs), regulate inflammatory profile their microenvironment CNS. It is now accepted that extracellular vesicle (EV) populations from act as mediators. Thus, collection isolation are important to identify contents but also evaluate biological effects on recipient cells....
Abstract Here we present an approach to identify N-linked glycoproteins and deduce their spatial localization using a combination of MALDI mass spectrometry N-glycan imaging spatially-resolved glycoproteomic strategies. We subjected formalin-fixed, paraffin-embedded glioma biopsies on-tissue PNGaseF digestion found that the glycan HexNAc4-Hex5-NeuAc2 was be predominantly expressed in necrotic regions high-grade canine gliomas, whereas high mannose HexNAc2-Hex5 benign regions. To determine...
Abstract The dogma “One gene, one protein” is clearly obsolete since cells use alternative splicing and generate multiple transcripts which are translated into protein isoforms, but also translation initiation sites termination on a given transcripts. Alternative open reading frames for individual give proteins (the Proteins: AltProts) originate from the 5'- 3'- UTR mRNA regions, frameshifts of ORFs or non-coding RNAs. To gain insight role these newly identified in regulation cellular...
Abstract Using multi-omics analyses including RNAseq, RT-PCR, RACE-PCR, and shotgun proteomic with enrichment strategies, we demonstrated that newborn rat astrocytes produce neural immunoglobulin constant variable heavy chains as well light chains. However, their edification is different from the ones found in B cells resembles aberrant immunoglobulins observed several cancers. Moreover, complete enzymatic V(D)J recombination complex has also been identified astrocytes. In addition, chain...