DNA methylation occurs in the displacement loop (D-loop) region of mammals; however, D-loop regions certain tumor tissue types were found to be de‑methylated. Whether hypomethylation D‑loop is involved regulation mitochondrial (mtDNA) copy number and nicotinamide adenine dinucleotide subunit 2 (ND‑2) expressions colorectal cancer has remained elusive. In present study, association between status region, mtDNA ND‑2 expression was investigated 65 specimens their corresponding non‑cancerous...

Betaine has previously been demonstrated to protect the liver against alcohol‑induced fat accumulation. However, mechanism through which betaine affects hepatic lipid metabolic disorders not extensively studied. The present study aimed investigate effect of on alcoholic simple fatty and metabolism disorders. A total 36 rats were randomly divided into control, ethanol + groups. Liver function, morphological alterations, content tumor necrosis factor (TNF)‑α levels determined. Hepatic...

Background Increased intra-hepatic resistance to portal blood flow is the primary factor leading hypertension in cirrhosis. Up-regulated expression of cyclooxygenase-2 (COX-2) cirrhotic liver might be a potential target ameliorate hypertension. Objective To verify effect celecoxib, selective inhibitor COX-2, on and mechanisms behind it. Methods Cirrhotic model rat was established by peritoneal injection thiacetamide (TAA). 36 rats were randomly assigned control, TAA TAA+celecoxib groups....

The epithelial-mesenchymal transition (EMT) of hepatocytes is a key step for hepatic fibrosis and cirrhosis. Long-term administration celecoxib, selective cyclooxygenase-2 (COX-2) inhibitor, can ameliorate fibrosis. This research aimed to examine the effect celecoxib on EMT during development liver cirrhosis.Cirrhotic model rat was established by peritoneal injection thiacetamide (TAA). Thirty-six rats were randomly assigned control, TAA, TAA + groups. Hepatic expressions tumor necrosis...

In colorectal cancer, no study has been carried out discovering the relationship among p53, mitochondrial transcription factor A (TFAM) expression and change of DNA (mtDNA) copy number. our study, co-expression p53 TFAM was observed in colon adenocarcinoma tissues, paracancerous tissues 9 cancer cell lines. Then, a significant linear correlation established between either or advanced TNM stage, positive lymph nodes low 5-year survival rate patients with adenocarcinoma. Additionally, large...

An alteration in the mitochondrial DNA (mtDNA) copy number has been detected numerous human cancers. However, certain changes mtDNA that occur during initiation and progression of gastric cancer remain undetected. In present study, using quantitative PCR analysis, were observed cancer. Furthermore, possible correlation between clinicopathological stage also investigated. mechanism by which change occurs remains to be elucidated. Epigenetic are believed play a significant role regulating...

It is not established whether de‑methylation of the displacement loop (D‑loop) region if mitochondrial DNA (mtDNA) directly influences mtDNA copy number and further alters cell cycle, apoptosis proliferation in colorectal cancer. The current study employed viability assays, cycle analysis, methylation analysis using 5 cancer lines. present results demonstrated that 5‑aza‑2'‑deoxycytidine (5‑AZA), a hypomethylating agent, significantly increased Lovo Colo‑205 In cells, proportion G0/G1 phase...

The analysis of differences in gene expression is dependent on normalization using reference genes. However, the many these genes, as evaluated by quantitative RT-PCR, upregulated acute pancreatitis, so they cannot be used standard for this condition. For reason, we sought to identify a stable gene, or suitable combination, pancreatitis. stability 10 genes (ACTB, GAPDH, 18sRNA, TUBB, B2M, HPRT1, UBC, YWHAZ, EF-1 α , and RPL-13A) was analyzed geNorm, NormFinder, BestKeeper software according...

Multiple long non-coding RNAs (lncRNAs) have been demonstrated to be involved in liver disease. Increased cyclooxygenase-2 (COX‑2) levels also reported the progression of cirrhosis. In present study, correlations between lncRNA‑COX‑2 RNA expression levels, COX‑2 mRNA and fibrosis were examined. Liver was induced by intraperitoneal injection carbon tetrachloride (CCl4) mice for 2 months (CCl4‑2M) or 3 (CCl4‑3M). histopathological evaluation conducted using hematoxylin eosin Masson trichrome...

Abstract AZD6244 (ARRY-142886), a highly selective MAPK-ERK kinase inhibitor, has shown excellent clinical efficacy in many tumors. However, the anti-tumor and anti-angiogenesis of on gastric cancer not been well characterized. In this study, high p-ERK expression was associated with advanced TNM stage, increased lymphovascular invasion poor survival. For absence NRAS, KRAS BRAF mutation, SGC7901 BGC823 cells were relative resistance to vitro . And such attributed insufficient inhibition ERK...

Among the researches on hepatic fibrosis, great attention was paid to both hepatocytes and extracellular matrix (ECM). However, little focus drawn reticular fibrous network, which is important for demarcation support of hepatocytes. The aim this study investigate change pattern fibers in fibrosis/cirrhosis its underlying mechanism. In study, thioacetamide (TAA) bile duct ligation (BDL) were utilized induce rat fibrosis respectively, Human liver cirrhotic microassay analyzed with IHC confirm...

Somatostatin and its analogues, which function by binding to somatostatin receptors (SSTRs) 1-5, play a protective role in liver cirrhosis. Hepatic SSTR-2 expression is up-regulated subjects with However, little known about the mechanisms underlying this process. In present study, we observed up-regulation of hepatic thioacetamide (TAA)-induced cirrhotic rats further showed that cyclooxygenase-2 (COX-2) might process via protein kinase C (PKC)-cAMP response element (CREB) signaling pathway....

To explore the effect of betaine on alcoholic pancreatic steatosis and its mechanism.Rats were randomly assigned to control, ethanol, or ethanol + groups. Changes in morphology; serum lipid levels; lipid, amylase lipase levels determined. The adipose tissue adiponectin level was measured by an enzyme-linked immunoassay. Adiponectin receptor-1 (AdipoR1), AdipoR2, sterol regulatory element binding protein-1c (SREBP-1c), SREBP-2, fatty acid synthetase expression quantified. SREBP-1c SW1990...

Ephedrine abuse has spread in many parts of the world and severely threatens human health. The mechanism ephedrine-induced toxicity still remains unclear. This study was performed to investigate effects ephedrine treatment on liver explore underlying mechanisms. Sprague Dawley rats were divided into saline groups. Rats treated with at 20 mg/kg or 40 ( n = 10) by oral gavage daily for 7 days. Pathological changes examined hematoxylin eosin staining terminal deoxynucleotidyl transferase...

The aim of the current study was to examine matrix metalloproteinase-2 (MMP-2) and tissue inhibitor (TIMP-2) expression in patients with cervical disc herniation (CDH). A total 127 specimens from CDH undergoing posterior spinal surgery were obtained for case group, which divided into three subgroups: lateral protrusion (N = 102), median 18), paramedian 7). Another 55 subjects who had spine trauma underwent canal decompression control group. Routine hematoxylin eosin staining performed...

Abstract Background Adipose stem cell‐derived exosomes (ADSC‐EXO) and botulinum toxin type A (BTX‐A) individually showed a therapeutic effect on skin wound repair. Aims This study investigated their synergistic promoting healing in vitro vivo the underlying molecular events. Methods ADSCs were isolated from Sprague–Dawley (SD) rats to obtain ADSC‐EXO by ultrafiltration ultracentrifugation confirmed using nanoparticle tracking analysis transmission electron microscopy. Human fibroblasts (HSF)...

To evaluate the encapsulation of VX2 hepatic allografts in rabbits induced by octreotide and celecoxib administration following transcatheter arterial embolisation (TAE), with were divided into four groups: control, TAE, + (O+C) multimodality therapy (TAE+O+C). Allograft metastasis, capsule thickness percentage clear cells measured vascular endothelial growth factor (VEGF) CD31 detected immunohistochemistry reverse transcription-polymerase chain reaction (RT-PCR) analysis. The extrahepatic...

Chronic inflammation is considered as an important pathophysiologic mechanism of hepatic cirrhosis, which induces hepatocyte injury and activates stellate cells (HSCs), thus resulting in fibrosis. Previous studies have reported that cyclooxygenase‑2 (COX‑2) inhibitor can effectively treat liver fibrosis, while somatostatin (SST) analogues inhibit the activation HSCs. The present study aimed to investigate effects a COX‑2 inhibitor, celecoxib, combined with SST analogue, octreotide, for...

3065 Background: We evaluated the safety of a combination bevacizumab (B) and TKI against VEGFR-cediranib (C). Methods: This was phase I study with 3+3 design in patients (pts) advanced solid tumors. C given po daily for 21 days B i.v. every two weeks (days 1 15). measured changes serum total nitrate response to therapy. Results: 51 pts were dosed. 2 at DL4 had DLT hypertension (HTN) grade 3 4. DL3 initially deemed MTD (C30 mg/day B5mg/kg/2weeks). However dose-expansion initial 18 pts, CNS...

2604 Background: The purpose of this study was to assess toxicity associated with investigational agents in patients advanced solid tumors treated a phase I clinic that focuses on targeted agents. Methods: An electronic database search/analysis cancer who presented the program between 1/1/2006 and 12/31/2008 performed. Results: Overall, 1,181 were identified: 893 (75.6%) pts only one clinical trial; 196 (16.6%) 2; 66 (5.6%) 3; 16 (1.4%) 4; 10 (<1%) 5 9 trials. median age 58 (range: 3-89...