A5050412833- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Protein Kinase Regulation and GTPase Signaling
- Reproductive Biology and Fertility
- Hypothalamic control of reproductive hormones
- Sperm and Testicular Function
- Estrogen and related hormone effects
- Computational Drug Discovery Methods
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Gene Regulatory Network Analysis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Nitric Oxide and Endothelin Effects
- RNA and protein synthesis mechanisms
- Growth Hormone and Insulin-like Growth Factors
- Ovarian function and disorders
- Neuroendocrine regulation and behavior
- interferon and immune responses
- Renin-Angiotensin System Studies
- Birth, Development, and Health
- Glycosylation and Glycoproteins Research
- Autism Spectrum Disorder Research
- Child Development and Digital Technology
- PI3K/AKT/mTOR signaling in cancer
- Hormonal and reproductive studies
Physiologie de la Reproduction et des Comportements
2016-2025
Université de Tours
2015-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Inria Saclay - Île de France
2021-2025
Université Paris-Saclay
2021-2025
Laboratoire de Tribologie et Dynamique des Systèmes
2020-2024
École Polytechnique
2020-2024
Centre National de la Recherche Scientifique
2014-2023
Institut Français du Cheval et de l'Équitation
2015-2023
Centre Val de Loire
2019
Abstract We have established transgenic mice expressing the Cre recombinase under control of anti‐Müllerian hormone (AMH) gene promoter. activity and specificity were evaluated by different means. In AMH‐ mice, expression mRNA was confined to testis ovary. crossed with reporter lines offspring exhibited Cre‐mediated recombination only in male, histochemical analysis indicated that occured every Sertoli cells. female, restricted granulosa cells, but protein not evenly active From these...
Classically, the FSH receptor (FSH-R) mediates its effects through coupling to guanine nucleotide-binding protein alpha S subunit (Galpha(s)) and activation of cAMP/protein kinase A (PKA) signaling pathway. beta-Arrestins are rapidly recruited FSH-activated play key roles in desensitization internalization. Here, we show that FSH-R expressed HEK 293 cells activated ERK by two temporally distinct pathways dependent, respectively, on Galpha(s)/PKA beta-arrestins. Galpha(s)/PKA-dependent was...
The I kappaB alpha protein is a key molecular target involved in the control of NF-kappaB/Rel transcription factors during viral infection or inflammatory reactions. This NF-kappaB-inhibitory factor regulated by posttranslational phosphorylation and ubiquitination its amino-terminal signal response domain that targets for rapid proteolysis 26S proteasome. In an attempt to identify regulators inhibitory activity, we undertook yeast two-hybrid genetic screen, using end as bait, identified 12...
In addition to their role in desensitization and internalization of G protein-coupled receptors (GPCRs), β-arrestins are essential scaffolds linking GPCRs Erk1/2 signaling. However, GPCR-operated activation differs between the underlying mechanism remains poorly characterized. Here, we show that serotonin 5-HT2C receptors, which engage pathway via a β-arrestin-dependent mechanism, promotes MEK-dependent β-arrestin2 phosphorylation at Thr383, necessary step for Erk recruitment...
Article26 June 2012Open Access Competing G protein-coupled receptor kinases balance protein and β-arrestin signaling Domitille Heitzler BIOS Group, INRA, UMR85, Unité Physiologie de la Reproduction et des Comportements, Nouzilly, France CNRS, UMR7247, Université François Rabelais, Tours, IFCE, Contraintes Team, INRIA Paris-Rocquencourt, Le Chesnay, Search for more papers by this author Guillaume Durand Nathalie Gallay Aurélien Rizk Seungkirl Ahn Department of Medicine, Duke University...
Gonadotropin receptors belong to the super family of G protein-coupled and mediate physiological effects follicle-stimulating hormone (FSHR) luteinizing (LHR). Their central role in control reproductive function has made them focus intensive studies. Upon binding their cognate hormone, they trigger complex signaling trafficking mechanisms that are tightly regulated concentration, time, space. Classical cellular assays often fail capture all these dynamics. Here, we describe use various...
Intracellular variable fragments from heavy-chain only antibodies of camelids (intra-VHH) have been successfully used for their stabilizing properties to solve the 3D structure active G protein-coupled receptors (GPCRs) bound cognate transducers. They also provide tools link a given conformation GPCR signalling network engaged, thus allowing extensive structure/activity studies. Recently, they instrumental in tracking GPCRs various subcellular compartments. Here, we report isolation and...
Abstract β-arrestins serve as signaling scaffolds downstream of G protein-coupled receptors and thus play a crucial role in plethora cellular processes. Although it is largely accepted that the ability to interact simultaneously with many protein partners key protein-independent GPCRs, only precise knowledge these multimeric arrangements will allow full understanding dynamics interactions their functional consequences. However, current experimental procedures for determination...
The organismal roles of the ubiquitously expressed class I PI3K isoform p110β remain largely unknown. Using a new kinase-dead knockin mouse model that mimics constitutive pharmacological inactivation p110β, we document full leads to embryonic lethality in substantial fraction mice. Interestingly, homozygous mice survive into adulthood (maximum ~26% on mixed genetic background) have no apparent phenotypes, other than subfertility females and complete infertility males. Systemic inhibition...
<title>Abstract</title> Autism spectrum disorders (ASD) are complex, polygenic and heterogenous neurodevelopmental conditions, imposing a substantial economic burden. ASD genetics is influenced by the environment, specifically social experience during critical period. Despite efficacy of early behavioral interventions targeting specific behaviors in some autistic children, there currently no sustainable treatment for two core symptoms: deficits interaction communication, stereotyped or...
Deglycosylated FSH is known to trigger poor Galphas coupling while efficiently binding its receptor. In the present study, we tested possibility that a deglycosylated equine LH (eLHdg) might be able selectively activate beta-arrestin-dependent signaling. We compared native eLH an derivative [i.e. truncated eLHbeta (Delta121-149) combined with asparagine56-deglycosylated eLHalpha (eLHdg)] previously reported as antagonist of cAMP accumulation at receptor (FSH-R). confirmed that, when used in...