A5040432322- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Animal Genetics and Reproduction
- Visual perception and processing mechanisms
- Memory and Neural Mechanisms
- Animal Vocal Communication and Behavior
- EEG and Brain-Computer Interfaces
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Animal Behavior and Reproduction
- Advanced Fluorescence Microscopy Techniques
- Neural and Behavioral Psychology Studies
- Marine animal studies overview
- Photoreceptor and optogenetics research
- Zebrafish Biomedical Research Applications
- Cell Image Analysis Techniques
- Neuroscience and Neural Engineering
- Receptor Mechanisms and Signaling
- Explainable Artificial Intelligence (XAI)
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Industrial Vision Systems and Defect Detection
- Neurogenesis and neuroplasticity mechanisms
- Visual Attention and Saliency Detection
- Anomaly Detection Techniques and Applications
BioInVision (United States)
2021-2024
Boston University
2021-2024
University of Oregon
2023
Princeton University
2010-2018
Neuroscience Institute
2015
Institute of Cognitive and Brain Sciences
2012
University of Chicago
2002-2010
Massachusetts Institute of Technology
2005-2009
South University
2005
London School of Economics and Political Science
1964
Decision-making behavior is often characterized by substantial variability, but its source remains unclear. We developed a visual accumulation of evidence task designed to quantify sources noise and be performed during voluntary head restraint, enabling cellular resolution imaging in future studies. Rats accumulated discrete numbers flashes presented the left right hemifields indicated side that had greater number flashes. Using signal-detection theory-based model, we found standard...
Summary A fundamental question in neuroscience is how memory formation shapes brain activity at the level of populations neurons. Recent studies hippocampal ‘engram’ cells, identified by immediate-early genes (IEGs) induced learning, propose that these act as a neuronal substrate for storage. The current framework engram proposes cells join ensembles based on increased intrinsic excitability, and after initial they co-activate to support retrieval. However, direct evidence population...
Birds are of great interest for a variety research purposes, and effective methods manipulating the avian genome would greatly accelerate progress in fields that rely on birds as model systems biological research, such developmental biology behavioral neurobiology. Here, we describe simple method producing transgenic birds. We used lentiviral vectors to produce quails express GFP driven by human synapsin gene I promoter. Expression was specific neurons consistent across multiple generations....
Zebra finches are widely used for studying the basic biology of vocal learning. The inability to introduce genetic modifications in these animals has substantially limited studies on molecular this behavior, however. We an HIV-based lentivirus produce germline transgenic zebra finches. encoded GFP regulated by human ubiquitin-C promoter [Lois C, Hong EJ, Pease S, Brown Baltimore D (2002) Science 295:868–872], which is active a wide variety cells. virus was injected into very early embryo...
Abstract Plans are formulated and refined over the period leading to their execution, ensuring that appropriate behavior is enacted at just right time. While existing evidence suggests memory circuits convey passage of time through diverse neuronal responses, it remains unclear whether neural involved in planning exhibit analogous temporal dynamics. Using publicly available data, we analyzed how activity frontal motor cortex evolves during planning. Individual neurons exhibited ramping...
The precise neural mechanisms within the brain that contribute to remarkable lifetime persistence of memory are not fully understood. Two-photon calcium imaging allows activity individual cells be followed across long periods, but conventional approaches require head-fixation, which limits type behavior can studied. We present a magnetic voluntary head-fixation system provides stable optical access during complex behavior. Compared previous systems used mechanical restraint, there no moving...
Plans are formulated and refined throughout the period leading up to their execution, ensuring that appropriate behaviors enacted at times. While existing evidence suggests memory circuits convey passage of time through diverse neuronal responses, it remains unclear whether neural involved in planning exhibit analogous temporal dynamics. Using publicly available data, we analyzed how activity mouse frontal motor cortex evolves during planning. Individual neurons exhibited ramping a delay...
Recording activity from large cell populations in deep neural circuits is essential for understanding brain function. Three-photon (3P) imaging an emerging technology that allows of structure and function subcortical structures. However, increased tissue heating, as well the low repetition rate sources inherent to 3P imaging, have limited fields view (FOV) areas ≤0.3 mm 2 . Here we present a Large Imaging Field (LIFT) microscope with FOV [gt]3 LIFT combines high numerical aperture (NA)...
Abstract New neurons are added to the forebrain song control regions high vocal center (HVC) and Area X of juvenile songbirds but identity site origin these cells have not been fully characterized. We used oncoretroviral vectors genetically label neuronal progenitors in different zebra finch lateral ventricle. A region corresponding mammalian medial ganglionic eminences generated medium spiny found striatum surrounding X, at least two classes interneurons HVC. In addition, our experiments...
Neuron migration is a key phase of neurogenesis, critical for the assembly and function neuronal circuits. In songbirds, this process continues throughout life, but how these newborn neurons disperse through adult brain unclear. We address question using in vivo two-photon imaging transgenic zebra finches that express GFP young other cell types. juvenile birds, migratory cells are present at high density, travel all directions, make frequent course changes. Notably, dynamic patterns well fit...
In this study we combine a novel pulse-based evidence accumulation task with newly developed motion-based drift diffusion model (mDDM). model, incorporate movement parameters derived from high-resolution video data to estimate of the on trial-by-trial basis. We find that new is an improved description animal choice behavior.
The ability to adapt the long-term aspects of chronic haemodialysis is multifactorial and poorly understood. Given many comorbidities a patient on haemodialysis, religious beliefs may be an important factor in patient's cope.End-stage renal disease patients inner-city American in-center unit were given two surveys quantify their quality life (KDQOL) (Royal Free Score). population studied included 97% African Americans. demographics collected recorded. relationship between religious/spiritual...
Most non-mammalian vertebrate species add new neurons to existing brain circuits throughout life, a process thought be essential for tissue maintenance, repair, and learning. How these migrate through the mature which cues trigger their integration within functioning circuit is not known. To address questions, we used two-photon microscopy image addition of genetically labeled newly generated into juvenile zebra finches. Time-lapse in vivo imaging revealed that majority migratory exhibited...
Optical imaging of calcium signals in the brain has enabled researchers to observe activity hundreds-to-thousands individual neurons simultaneously. Current methods predominantly use morphological information, typically focusing on expected shapes cell bodies, better identify field-of-view. The explicit shape constraints limit applicability automated identification other important scales with more complex morphologies, e.g., dendritic or widefield imaging. Specifically, fluorescing...
Abstract Perceptual decision-making involves multiple cognitive processes, including accumulation of sensory evidence, planning, and executing a motor action. How these processes are intertwined is unclear; some models assume that decision-related precede execution, whereas others propose movements reflecting on-going decision occur before commitment to choice. Here we develop apply two complementary methods study the relationship between leading up The first free response pulse-based...
Cross-species studies are important for a comprehensive understanding of brain functions. However, direct quantitative comparison behaviors across species presents significant challenge. To enable such comparisons in perceptual decision-making, we developed synchronized evidence accumulation task human and non-human animals, by aligning mechanics, stimuli, training. The was readily learned rats, mice humans, with each exhibiting qualitatively similar performance. Quantitative model revealed...
• Development of a kinematic clamp for mice precise repositioning in chronic studies. Headplate and provide stability cellular resolution imaging during behavior. Ruby contact features enable submicron registration repeatability.