Abstract Background Mangrove forests are coastal wetlands that provide vital ecosystem services and serve as barriers against natural disasters like tsunamis, hurricanes tropical storms. Mangroves harbour a large diversity of organisms, including microorganisms with important roles in nutrient cycling availability. Due to tidal influence, mangroves sites where crude oil from spills farther away can accumulate. The relationship between mangrove bacterial degradation sediments remains poorly...

Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly Staphylococcus aureus and Pseudomonas aeruginosa, with the ability to form biofilms. This study aimed evaluate colonization biofilm formation of aeruginosa in chronic wounds diabetic treated a bioactive dressing (EGF-CMC), consisted 2% carboxymethylcellulose (CMC) hydrogel loaded epidermal growth factor (EGF). randomized clinical trial was conducted 25 participants: 14 EGF-CMC 11 CMC for 12 weeks....

We analysed the antimicrobial susceptibility, biofilm formation and genotypic profiles of 27 isolates Staphylococcus haemolyticus obtained from blood 19 patients admitted to a hospital in Rio de Janeiro, Brazil. Our analysis revealed clinical significance 36.8% multi-resistance rate 92.6% among these isolates. All but one isolate carried mecA gene. The staphylococcal cassette chromosome mec type I was most prevalent element detected (67%). Nevertheless, showed clonal diversity based on...

In this study, 100 clinical isolates of Streptococcus agalactiae recovered from genitourinary tract specimens non-pregnant individuals living in Rio de Janeiro were submitted for antimicrobial susceptibility testing, detection macrolide resistance genes and evaluation the genetic diversity erythromycin-resistant isolates. By agar diffusion method, all susceptible to ceftazidime, penicillin vancomycin. Isolates resistant levofloxacin (1%), clindamycin (5%), erythromycin (11%) tetracycline...

This study aimed to investigate classical enterotoxin (sea see) and mecA genes, by polymerase chain reaction anitimicrobial susceptibility, disk diffusion test of Staphylococcus aureus isolated from minas frescal cheese (MFC). All methicillin-resistant S. (MRSA) isolates were investigated for the presence Panton-Valentine leukocidin (PVL) genes clonal diversity. Thirty-one four MFC samples. Seven (22.6%) carried gene two them seb/sec sea/seb. Five (16.1%) showed induced resistance...

Acinetobacter spp. have emerged as notorious pathogens involved in healthcare-associated infections. Carbapenems are important antimicrobial agents for treating infections due to multidrug resistant Different mechanisms may confer resistance these drugs the genus, particularly production of class D carbapenemases. OXA-23-like family has been pointed out one predominant carbapenamases among Acinetobacter. The present work aimed investigate occurrence carbapenemases isolates recovered from...

Background: Streptococcus agalactiae is known to be the major cause of neonatal infections and also causes complications during pregnancy. Methods: One hundred six strains recovered from clinical specimens newborns (n = 18) pregnant women 88) were submitted antimicrobial susceptibility testing investigation genetic determinants macrolide resistance, capsular type, virulence factors. Genetic diversity was evaluated by pulsed-field gel electrophoresis (PFGE) analysis. Results: Strains...

Our purposes in this study were to (1) identify Pseudomonas aeruginosa strains collected from swabs of chronic wounds, (2) evaluate the susceptibility P. various antimicrobials, (3) detect presence virulence factors exoenzyme S (exoS) and U (exoU) strains, (4) wound colonization by via pulsed-field gel electrophoresis (PFGE).Descriptive research using a quantitative approach.Swabs 43 adults with wounds treated an outpatient setting Niterói City, Brazil, included convenience sampling.Swabs at...

Methicillin-resistant Staphylococcus epidermidis (MRSE) and methicillin-resistant haemolyticus (MRSHa) are important coagulase-negative staphylococci. They often isolated from bacteremia in humans mainly due to their ability form biofilm on the surfaces of medical devices. Papain is a complex mixture proteolytic enzymes peroxidases extracted latex Carica papaya it recognized by accelerating healing process wounds. This study aimed evaluate MRSE MRSHa isolates produce biofilms. Besides this,...

INTRODUCTION In recent decades, the prevalence of carbapenem-resistant Acinetobacter isolates has increased, and production oxacillinase (OXA)-type carbapenemases is main mechanism underlying resistance. We evaluated OXA from 114 collected between March December 2013 different clinical specimens patients in two hospitals (Hospital 1 [n = 61] Hospital 2 53]) located Niterói, Rio de Janeiro, Brazil. also genetic diversity OXA-producing isolates. METHODS All were identified through automated...

Three isolates of Streptococcus agalactiae, recovered from residents the metropolitan area Rio de Janeiro with significant bacteriuria, were found to be resistant levofloxacin. Determination minimal inhibitory concentration (MIC) confirmed one isolate as intermediate and two No reduction in levofloxacin MIC was observed reserpine, indicating that resistance not caused by an efflux mechanism. Typical point mutations quinolone determinant region gyrA parC. Other parC generated novel altered...

Researchers analyzed chronic wounds treated with 2% hydrogel to determine whether the presence of methicillin-resistant Staphylococcus aureus (MRSA) is related clinical signs infection.Thirty-five patients were recruited for this descriptive study using a quantitative approach. was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Antibiotic susceptibility determined disk diffusion test according Clinical and Laboratory Standards Institute standards....

The objective was to evaluate Staphylococcus aureus and Pseudomonas aeruginosa colonisation of wounds treated with recombinant epidermal growth factor (EGF) platelet-rich plasma (PRP); analyse the susceptibility profiles S. P. isolates from EGF PRP; describe presence infection in EGF-treated PRP-treated wounds. Experimental study performed using clinical specimens collected swabs. Patients were PRP outpatient clinic a university hospital. Forty-three obtained 31 patients, 41.9% (13/31) whom...

Study the use of magistral oral solutions and suspensions in infants children at a university hospital. This is descriptive study based on analysis assessed hospital's drug request forms regarding patients neonatal ICU, Obstetrics, Pediatrics Pediatric Emergency from January 2012 to December 2013. The frequency requests dispensation was evaluated consumption each active ingredient preparations expressed as number "infant defined daily dose" (iDDD) iDDD/100 bed-days. A total 657 were analyzed...

Quorum sensing is a cell–cell signaling mechanism based on cell density and that involves the production of hormone‐like molecules called autoinducers (AI). One most studied AIs has been termed AI‐2, its biosynthesis requires enzyme encoded by luxS . We have previously described for first time Bacteroides species can produce with AI‐2 activity. In this study, we focus detection activity as synthase in species. The strains fragilis B3b vulgatus ATCC 8482 were selected positive phenotype...

Streptococcus dysgalactiae subsp. equisimilis (SDSE) has been increasingly associated with several infectious diseases, ranging from pharyngitis to life-threatening conditions, such as necrotizing fasciitis and streptococcal toxic shock syndrome. However, its molecular epidemiology in some geographical areas remains unclear.In this study, 44 isolates of SDSE, recovered noninvasive infections (37) carriage (7), during 2008-2013, were submitted antimicrobial susceptibility testing, emm typing...