Staphylococcus aureus isolates from five large teaching hospitals and one medium-size community hospital located in geographically distant parts of Brazil, the south southeast (Rio de Janeiro, Niteroi, Sao Paulo, Porto Alegre) north (Manaus), were tested for their antibiotic resistance patterns genetic backgrounds. Eighty-five 152 identified as methicillin-resistant S. (MRSA) by using a combination an agar dilution screen mecA gene-specific DNA probe. All MRSA resistant to penicillin,...

Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a therapeutic problem. In the present study, molecular characterization by pulsed-field gel electrophoresis of MRSA isolates collected from university hospital revealed that predominant variant Brazilian epidemic clonal complex (BECC) was responsible for increase in incidence strains, which reached 28% 1998. It verified this BECC displayed an enhanced ability to produce biofilm on inert polystyrene surfaces and adhere invade...

Objectives: To study biofilm production and to detect icaAD, atlE aap genes in 137 isolates of methicillin-resistant Staphylococcus epidermidis (MRSE) obtained from healthy individuals the community (35 isolates), hospitalized patients at Antônio Pedro University Hospital (25 isolates) a home-care system (HCS; 77 isolates).

The present study aimed at investigating the chemical composition of essential oil extracted from Brazilian propolis and susceptibility Staphylococcus aureus, epidermides, Streptococcus pyogenes Escherichia coli to this substance. was obtained by steam distillation examined gas chromatography/mass spectrometry (GC/MS). In addition, agar diffusion method using filter paper disks employed. Antibacterial activity measured as equivalent diameters inhibition zones (in millimeters) after...

Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly Staphylococcus aureus and Pseudomonas aeruginosa, with the ability to form biofilms. This study aimed evaluate colonization biofilm formation of aeruginosa in chronic wounds diabetic treated a bioactive dressing (EGF-CMC), consisted 2% carboxymethylcellulose (CMC) hydrogel loaded epidermal growth factor (EGF). randomized clinical trial was conducted 25 participants: 14 EGF-CMC 11 CMC for 12 weeks....

We analysed the antimicrobial susceptibility, biofilm formation and genotypic profiles of 27 isolates Staphylococcus haemolyticus obtained from blood 19 patients admitted to a hospital in Rio de Janeiro, Brazil. Our analysis revealed clinical significance 36.8% multi-resistance rate 92.6% among these isolates. All but one isolate carried mecA gene. The staphylococcal cassette chromosome mec type I was most prevalent element detected (67%). Nevertheless, showed clonal diversity based on...

Seventy-two penicillin-resistant Streptococcus pneumoniae isolates collected from clinical specimens in the Czech and Slovakian Republics between 1990 1992 were analyzed by a variety of molecular techniques. Most highly resistant (40/72) (penicillin MIC 1 up to 16 μg/ml) represented two distinct pneumococcal clones, most these (35/40) also at least other antibiotics (tetracycline plus chloramphenicol or erythromycin). All 17 belonging first clone serotype 14, had very high penicillin MICs...

Usually, community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is susceptible to a variety of non-beta-lactam drugs. These isolates commonly display SCCmecIV and are associated with infections. More recently, CA-MRSA has been isolated from health-care-associated diseases. We characterized MRSA 2 hospitals in Rio de Janeiro area assess the entry new lineages. The were primary genotyped using combination molecular typing methods including SCCmec, restriction modification...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emergent pathogen in Brazil. However, there are no data on the prevalence of CA-MRSA. We report here first well-characterized case severe life-threatening CA-MRSA infection a child living Rio de Janeiro city. The patient had many complications including hematogenous osteomyelitis and involvement multiple sites requiring drainage soft-tissue abscess, pleural pericardial empyema. MRSA isolates recovered were...

Methicillin-resistant Staphylococcus aureus (MRSA) is recognised as an important cause of nosocomial infection. The spread some MRSA epidemic clones well documented. In Brazil, and more recently in Portugal, a considerable number hospital infections has been caused by unique multiresistant clone designated the Brazilian clone. This paper describes this hospitals two cities Argentina.

HEXANIC EXTRACT FROM Hypericum brasilienseChoysi.Three phloroglucinols were obtained from brasiliense: japonicine A (1), isouliginosin B (2) and uliginosin (3). Bioautography disk diffusion methods used to determine antibacterial activity of the hexanic extract.Strains Coagulase Negative Staphylococcus American Methicillin Resistant aureus clones showed a growth inhibition zone ranging 10 12 mm 7 15 mm, respectively.Minimal inhibitory concentration (MIC) values measure antistaphylococcal for...

This study aimed to investigate classical enterotoxin (sea see) and mecA genes, by polymerase chain reaction anitimicrobial susceptibility, disk diffusion test of Staphylococcus aureus isolated from minas frescal cheese (MFC). All methicillin-resistant S. (MRSA) isolates were investigated for the presence Panton-Valentine leukocidin (PVL) genes clonal diversity. Thirty-one four MFC samples. Seven (22.6%) carried gene two them seb/sec sea/seb. Five (16.1%) showed induced resistance...

In this paper we carried out a study about prevalence of the clinically significant coagulase negative staphylococcal (CNS) isolates found in an university hospital. Two hundred four CNS from 191 patients obtained between period 1998 to 2002, were studied. About 27% (52/191) infection cases studied confirmed as CNS-associated diseases. Blood stream (BSI) was most frequent associated-disease (25%; 13/52). The great majority BSI verified Neonatal Intensive Care Unit (NICU). analysis 52 medical...

Our purposes in this study were to (1) identify Pseudomonas aeruginosa strains collected from swabs of chronic wounds, (2) evaluate the susceptibility P. various antimicrobials, (3) detect presence virulence factors exoenzyme S (exoS) and U (exoU) strains, (4) wound colonization by via pulsed-field gel electrophoresis (PFGE).Descriptive research using a quantitative approach.Swabs 43 adults with wounds treated an outpatient setting Niterói City, Brazil, included convenience sampling.Swabs at...

The extensive geographic spread of MRSA isolates belonging to the Brazilian epidemic clone (BEC) limited value pulsed-field gel electrophoresis (PFGE) in epidemiological studies outbreaks caused by these strains. Thus, discriminatory power eight different molecular methods was evaluated an attempt establish a methodology for genotyping BEC involved intra-hospital outbreaks. from five hospitals Teresina City, Piaui State were genotyped conventional or PFGE Cla I- Sma I-digested genomic DNA...

Methicillin-resistant Staphylococcus epidermidis (MRSE) and methicillin-resistant haemolyticus (MRSHa) are important coagulase-negative staphylococci. They often isolated from bacteremia in humans mainly due to their ability form biofilm on the surfaces of medical devices. Papain is a complex mixture proteolytic enzymes peroxidases extracted latex Carica papaya it recognized by accelerating healing process wounds. This study aimed evaluate MRSE MRSHa isolates produce biofilms. Besides this,...

Neste trabalho avaliou-se a atividade antibacteriana e IMAO de extratos diferentes polaridades Mikania glomerata. A foi medida frente à cepa multiresistente Staphylococus aureus PI57, através das técnicas bioautografia antibiograma. utilizando uma suspensão mitocôndrias. glomerata mostrou conter no extrato hexânico substâncias antimicrobianas. Os CH2Cl2 foram ativos MAO-B, sem apresentarem inibição da MAO-A, enquanto o metanólico apresentou MAO-A seletividade.

Summary Mupirocin is a topical antimicrobial agent that has been sucessfully used to eradicate methicillin-resistant Staphyiococcus aureus from the anterior nares and other sites of patients health care personnel. This report describes acquisition novel mupirocin resistance gene (ileS) by an epidemic MRSA clone geographically widespread in Brazil.

Researchers analyzed chronic wounds treated with 2% hydrogel to determine whether the presence of methicillin-resistant Staphylococcus aureus (MRSA) is related clinical signs infection.Thirty-five patients were recruited for this descriptive study using a quantitative approach. was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Antibiotic susceptibility determined disk diffusion test according Clinical and Laboratory Standards Institute standards....

The objective was to evaluate Staphylococcus aureus and Pseudomonas aeruginosa colonisation of wounds treated with recombinant epidermal growth factor (EGF) platelet-rich plasma (PRP); analyse the susceptibility profiles S. P. isolates from EGF PRP; describe presence infection in EGF-treated PRP-treated wounds. Experimental study performed using clinical specimens collected swabs. Patients were PRP outpatient clinic a university hospital. Forty-three obtained 31 patients, 41.9% (13/31) whom...