A5010399396- Mast cells and histamine
- Cell Adhesion Molecules Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Food Allergy and Anaphylaxis Research
- Polyamine Metabolism and Applications
- Receptor Mechanisms and Signaling
- Extracellular vesicles in disease
- Asthma and respiratory diseases
- Cellular transport and secretion
- Ion Channels and Receptors
- Cancer-related gene regulation
- HIV Research and Treatment
- Click Chemistry and Applications
- Viral Infections and Vectors
- Fungal Infections and Studies
- Reproductive System and Pregnancy
- Vector-Borne Animal Diseases
- Hippo pathway signaling and YAP/TAZ
- Chemokine receptors and signaling
- Zebrafish Biomedical Research Applications
- Mosquito-borne diseases and control
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Adenosine and Purinergic Signaling
- Monoclonal and Polyclonal Antibodies Research
Universidade de São Paulo
2013-2021
National Institutes of Health
2020
National Institute of Dental and Craniofacial Research
2020
Nef is an HIV-1 accessory protein that promotes viral replication and pathogenesis. A key function of to ensure sustained depletion CD4 MHC-I molecules in infected cells by inducing targeting these proteins multivesicular bodies (MVBs), ultimately lysosomes for degradation. also affects cellular secretory routes promoting its own secretion via exosomes. To better understand the effects on exocytic pathway, we investigated whether this factor modifies composition exosomes released T...
Peribunyaviridae is a large family of RNA viruses with several members that cause mild to severe diseases in humans and livestock. Despite their importance public heath very little known about the host cell factors hijacked by these support assembly egress. Here we show Oropouche virus, member genus Orthobunyavirus causes frequent arboviral infection South America countries, involves budding virus particles toward lumen Golgi cisternae. As viral replication progresses, subcompartments become...
Background The fungus Paracoccidioides brasiliensis is the leading etiological agent of paracoccidioidomycosis (PCM), a systemic granulomatous disease that typically affects lungs. Cell wall components P. interact with host cells and influence pathogenesis PCM. In yeast, many glycosylphosphatidylinositol (GPI)-anchored proteins are important in initial contact host, mediating host-yeast interactions culminate disease. PbPga1 GPI anchored protein located on surface yeast recognized by sera...
Mast cells are immunoregulatory that participate in inflammatory processes. Cross-linking mast cell specific GD1b derived gangliosides by mAbAA4 results partial activation of without the release preformed mediators. The present study examines newly formed and synthesized mediators following ganglioside cross-linking. with released lipid mediators, prostaglandins D 2 E , leukotrienes B 4 C . effect cross-linking these on enzymes arachidonate cascade was then investigated. Ganglioside resulted...
Oral squamous cell carcinoma (OSCC) remains a challenging cancer to treat despite all the advances of last 50 years. Kallikrein 5 (KLK5) is among serine proteases implicated in OSCC development. However, whether activity KLK5 promotes carcinogenesis still controversial. Moreover, knowledge regarding role cognate inhibitor, Lympho-Epithelial Kazal-Type related Inhibitor (LEKTI), scarce. We have, thus, sought investigate importance and LEKTI expression premalignant malignant lesions oral...
Mast cells are multifunctional immune that participate in many important processes such as defense against pathogens, allergic reactions, and tissue repair. These perform their functions through the release of a wide variety mediators. This occurs mainly cross-linking IgE (immunoglobulin E) bound to high affinity receptors by multivalent antigens. The abundance mast connective tissue, surrounding blood vessels, involvement early stages bone repair support possibility physiological...
ABSTRACT Although RACK1 is known to act as a signaling hub in immune cells, its presence and role mast cells (MCs) undetermined. MC activation via antigen stimulation results mediator release preceded by cytoskeleton reorganization Ca2+ mobilization. In this study, we found that was distributed throughout the cytoplasm both vivo vitro. After knockdown (KD), MCs were rounded, cortical F-actin fragmented. Following stimulation, KD MCs, there reduction F-actin, an increase monomeric G-actin...
Mast cells originate from pluripotent hematopoietic stem cells. Two mast cell specific antibodies, mAbsAA4 and BGD6, have previously been used to identify study committed precursors (MCcps) in the bone marrow of adult mice rats. However, embryonic origin MCcps is still not known. In present study, we identified rat embryos using these characterized antibodies. The were found AGM (aorta-gonad-mesonephros) region at E11.5. These BGD6+, CD34+, c-kit+, CD13+, FcεRI−, AA4− CD40−, Thy-1−. By PCR...
Mast cell (MC) secretory granules are Lysosome-Related Organelles (LROs) whose biogenesis is associated with the post-Golgi and endocytic pathways in which sorting of proteins destined for a specific organelle relies on recognition signals by adaptor that direct their incorporation into transport vesicles. The protein 3 (AP-3) complex mediates trafficking between trans-Golgi network (TGN) late endosomes, lysosomes, LROs. AP-3 has recognized role LROs regulated secretion several types,...
Rats were injected with rat recombinant (rr) IL3, rrSCF, rrIL-3 + rrRANTES and LTB4. Six hours after subcutaneous injection of or there was an increase in mast cell numbers the skin spleen. Peritoneal cells recruited following i.p. rrIL-3, but rrSCF recruitment delayed. Immunostaining a specific antibody showed that immature orthochromatic being recruited. induced to peritoneal cavity blocked by anti-integrin antibodies. Mast depended on target tissue time exposure chemoattractant.
Head and neck squamous cell carcinoma remains challenging to treat with no improvement in survival rates over the past 50 years. Thus, there is an urgent need discover more reliable therapeutic targets biomarkers for HNSCC. Matriptase, a type-II transmembrane serine protease, induces malignant transformation epithelial stem cells through proteolytic activation of pro-HGF PAR-2, triggering PI3K-AKT-mTOR NFKB signaling. The protease inhibitor lympho-epithelial Kazal-type-related (LEKTI)...
Phospholipase D (PLD) hydrolyses phosphatidylcholine to produce phosphatidic acid (PA) and choline. It has two isoforms, PLD1 PLD2, which are differentially expressed depending on the cell type. In mast cells it plays an important role in signal transduction. The aim of present study was clarify PLD2 secretory pathway. RBL-2H3 cells, a line, transfected overexpress catalytically active (PLD2CA) inactive (PLD2CI) forms were used. Previous observations showed that Golgi complex well organized...