A5019824695- Hippo pathway signaling and YAP/TAZ
- Receptor Mechanisms and Signaling
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Hedgehog Signaling Pathway Studies
- Cell Adhesion Molecules Research
- Wound Healing and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Cancer Genomics and Diagnostics
- Oral health in cancer treatment
- Immunotherapy and Immune Responses
- Cellular Mechanics and Interactions
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Cancer-related gene regulation
- Protein Kinase Regulation and GTPase Signaling
- Plant Surface Properties and Treatments
- RNA modifications and cancer
- Genetic and rare skin diseases.
- Toxin Mechanisms and Immunotoxins
- Fibroblast Growth Factor Research
- RNA Interference and Gene Delivery
National Cancer Institute
2017-2025
Center for Cancer Research
2017-2025
National Institutes of Health
2015-2025
National Institute of Dental and Craniofacial Research
2010-2018
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2016-2018
Tel Aviv University
2018
Sichuan University
2014
Universidad Nacional de Córdoba
2004-2013
Consejo Nacional de Investigaciones Científicas y Técnicas
2011
Research Centre in Biological Chemistry of Córdoba
2011
Transcriptional profiling of human cutaneous and oral wound healing reveals pathways involved in rapid resolution.
Abstract Dysregulation of the Hippo signaling pathway and consequent YAP1 activation is a frequent event in human malignancies, yet underlying molecular mechanisms are still poorly understood. A pancancer analysis core kinases their candidate regulating molecules revealed few alterations canonical pathway, but very genetic FAT family atypical cadherins. By focusing on head neck squamous cell carcinoma (HNSCC), which displays FAT1 (29.8%), we provide evidence that functional loss results...
Abstract Summary: Two studies published in this issue of Cancer Discovery describe the emerging mutational landscape head and neck squamous cell carcinomas (HNSCC) their genomic epigenetic alterations, thus identifying novel actionable cancer drivers predictive biomarkers for targeted therapies. Most alterations HNSCC converge a handful molecular pathways, resulting cell-cycle deregulation, instability, differentiation defects, persistent mitogenic signaling, latter involving aberrant...
Cetuximab, a monoclonal blocking antibody against the epidermal growth factor receptor EGFR, has been approved for treatment of squamous cell carcinomas head and neck (HNSCC). However, only few patients display long-term responses, prompting search cetuximab resistance mechanisms new therapeutic options enhancing effectiveness. Cetuximab-sensitive HNSCC cells were retro-engineered to express PIK3CA RAS oncogenes. These harboring endogenous oncogenes xenografted into mice (n = 10 per group)...
Abstract The Hippo TEAD-transcriptional regulators YAP1 and TAZ are central for cell renewal cancer growth; however, the specific downstream gene networks involved in their activity not completely understood. Here we introduce TEADi, a genetically encoded inhibitor of interaction with TEAD, as tool to characterize transcriptional biological effects regulated by TEAD transcription factors. Blockage TEADi human keratinocytes mouse skin leads reduced proliferation rapid activation...
Significance We generated a novel conditional inducible mouse model of fibrous dysplasia (FD) by expressing mutant GNAS in skeletal stem cells (SSCs) temporally controlled and tissue-specific fashion. Typical FD bone lesions developed rapidly embryos postnatal mice. promoted PKA activation proliferation SSCs along the osteogenic lineage but impaired their differentiation to mature osteoblasts triggered increased osteoclastogenesis resorption. reverted on cessation expression. Thus, mutation...
Basal cell carcinoma (BCC), the most common human cancer, is driven by hyperactivation of Hedgehog Smoothened (SMO) and GLI transcription. Gαs protein kinase A (PKA) negatively regulate signaling, offering an alternative BCC development treatment pathway. Here, using histology alongside bulk single-cell RNA sequencing, we find that mouse BCC-like tumors originate from pathway inactivation are strikingly similar to those canonical SMO. Interestingly, mutations reduce PKA activity present in...
The rising incidence of human papillomavirus (HPV)-associated malignancies, especially for oropharyngeal cancers, has highlighted the urgent need to understand how interplay between high-risk HPV oncogenes and carcinogenic exposure results in squamous cell carcinoma (SCC) development. Here, we describe an inducible mouse model expressing high risk HPV-16 E6/E7 oncoproteins adults, bypassing impact these viral genes during were targeted basal epithelia transgenic mice using a doxycycline...
The PKA-inhibitor (PKI) family members PKIα, PKIβ, and PKIγ bind with high affinity to PKA block its kinase activity, modulating the extent, duration of PKA-mediated signaling events. While is a well-known regulator physiological oncogenic events, role PKI proteins in these pathways has remained elusive. Here, by measuring activation MAPK pathway downstream GPCR-Gαs-cAMP signaling, we show that expression levels can alter balance two major cAMP targets: EPAC. Our results indicate maintains...
Objective Photosensitivity is one of the most common manifestations systemic lupus erythematosus (SLE), yet its pathogenesis not well understood. The normal‐appearing epidermis patients with SLE exhibits increased ultraviolet B (UVB)–driven cell death that persists in culture. Here, we investigated role epigenetic modification and Hippo signaling enhanced UVB‐induced apoptosis seen keratinocytes. Methods We analyzed DNA methylation cultured keratinocytes from compared to healthy controls (n...
Recently portable Raman probes have emerged along with a variety of applications, including carbon nanotube (CNT) characterization.Aqueous dispersed CNTs shown promise for biomedical applications such as drug/gene delivery vectors, photo-thermal therapy, and photoacoustic imaging.In this study we report the simultaneous detection irradiation nanotubes in 2D monolayers cancer cells 3D spheroids using probe.A handheld instrument was utilized dual purposes: CNT detector an irradiating laser...
Article12 March 2019Open Access Transparent process Genome-wide prediction of synthetic rescue mediators resistance to targeted and immunotherapy Avinash Das Sahu Corresponding Author [email protected] orcid.org/0000-0002-2193-6276 Department Biostatistics Computational Biology, Harvard School Public Health, Boston, MA, USA Medicine Medical School, Massachusetts General Hospital Cancer Center, University Maryland Institute Advanced Computer Science (UMIACS), Maryland, College Park, MD,...
// Zhao Zhu 1 , Baoxing Pang Ramiro Iglesias-Bartolome 2 Xiaoshan Wu Lei Hu Chunmei Zhang Jinsong Wang J. Silvio Gutkind 3 Songlin Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key of Regeneration Function Reconstruction, Capital Medical University School Stomatology, 100050, China Oral Pharyngeal Cancer Branch, National Institute Dental Craniofacial Research, Institutes Health, Bethesda, MD 20852, USA Department Pharmacology Moores Center, UC San Diego, La Jolla, CA...