A5080494751- Lysosomal Storage Disorders Research
- Cellular transport and secretion
- Carbohydrate Chemistry and Synthesis
- Calcium signaling and nucleotide metabolism
- Lipid Membrane Structure and Behavior
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Autophagy in Disease and Therapy
- Trypanosoma species research and implications
- Toxoplasma gondii Research Studies
- Sphingolipid Metabolism and Signaling
- Tuberous Sclerosis Complex Research
- PI3K/AKT/mTOR signaling in cancer
- Sulfur Compounds in Biology
- Polyamine Metabolism and Applications
- Parasitic Infections and Diagnostics
- Microbial Metabolic Engineering and Bioproduction
- Cholinesterase and Neurodegenerative Diseases
- Immune Cell Function and Interaction
- Nanoplatforms for cancer theranostics
- Cell death mechanisms and regulation
- MicroRNA in disease regulation
- Histiocytic Disorders and Treatments
- Advanced Drug Delivery Systems
Associazione Italiana Sclerosi Multipla
2023
University of Perugia
2011-2020
University of Udine
2013-2014
European Institute of Oncology
2013
Exosomes are small extracellular vesicles (30-100 nm) derived from the endosomal system, which have raised considerable interest in last decade. Several studies shown that they mediate cell-to-cell communication a variety of biological processes. Thus, addition to direct interaction or secretion active molecules, now considered another class signal mediators. can be secreted by several cell types and retrieved many body fluids, such as blood, urine, saliva cerebrospinal fluid. In proteins...
Lysosomes are cytoplasmic organelles present in almost all eukaryotic cells, which play a fundamental role key aspects of cellular homeostasis such as membrane repair, autophagy, endocitosis and protein metabolism. The characterization the genes enzymes constituting lysosome represents central issue to be addressed toward better understanding biology this organelle. In humans, mutations that cause lysosomal enzyme deficiencies result >50 different disorders severe pathologies. So far, many...
BACKGROUND Protein hydrolysates or hydrolysed proteins (HPs) are high-N organic fertilizers allowing the recovery of by-products (leather meal and fluid proteins) otherwise disposed as polluting wastes, thus enhancing matter energy conservation in agricultural systems while decreasing potential pollution. Chemical biological characteristics HPs animal origin were analysed this work to assess their safety, environmental sustainability efficacy fertilizers. Different obtained by thermal,...
Recently, the use of mammalian target rapamycin (mTOR) inhibitors, in particular (Rp), has been suggested to improve treatment neurodegenerative diseases. However, as Rp is a strong immunosuppressant, specific delivery brain postulated avoid systemic exposure. In this work, we fabricated new loaded solid lipid nanoparticles (Rp-SLN) stabilized with polysorbate 80 (PS80), comparing two different methods and lipids. The formulations were characterized by differential scanning calorimetry...
Loss-of-function mutations in the KRIT1 gene are associated with pathogenesis of cerebral cavernous malformations (CCMs), a major cerebrovascular disease still awaiting therapies. Accumulating evidence demonstrates that plays an important role redox-sensitive mechanisms, including transcriptional pathways and autophagy, which play roles cellular homeostasis defense against oxidative stress, raising possibility loss has pleiotropic effects on multiple systems. Using previously established...
The circumsporozoite protein (CSP) plays a key role in malaria sporozoite infection of both mosquito salivary glands and the vertebrate host. conserved Regions I II have been well studied but little is known about immunogenic central repeat region N-terminal protein. Rodent Plasmodium berghei parasites, which endogenous CS gene has replaced with avian gallinaceum (PgCS) sequence, develop normally A. stephensi midgut sporozoites are not infectious. We therefore generated P. transgenic...
Summary The intracellular parasite T oxoplasma gondii converts from a rapidly replicating tachyzoite form during acute infection to quiescent encysted bradyzoite stage that persists inside long‐lived cells chronic infection. Bradyzoites adopt reduced metabolism and slow replication while waiting for an opportunity recrudesce the within host. Interconversion between these two developmental stages is characterized by expression of glycolytic isoenzymes play key roles in metabolism. genome...
Mature β‐hexosaminidase A has been found associated to the external leaflet of plasma membrane cultured fibroblasts. The association directly determined by cell surface biotinylation followed affinity chromatography purification biotinylated proteins, and immunocytochemistry. immunological biochemical characterization revealed that enzyme is fully processed, suggesting its lysosomal origin.
H-Ras oncogene requires deregulation of additional oncogenes or inactivation tumor suppressor proteins to increase cell proliferation rate and transform cells. In fact, the expression constitutively activated H-RasV12 induces growth arrest premature senescence, which act like barriers in pre-neoplastic lesions. our experimental model, human fibroblasts transfected with show a dramatic modification morphology. expressing cells also senescence followed by death, induced autophagy apoptosis....
Glycogenosis type II, or Pompe Disease, is a lysosomal storage disease caused by the deficiency of acid alpha-glucosidase (GAA), leading to glycogen accumulation in muscles. A recombinant human GAA (rhGAA, Myozyme®) currently used for enzyme replacement therapy. Despite its efficacy most patients, some them show diminished response treatment with rapidly progressive clinical deterioration, due immuno-mediated inactivation. To demonstrate that Nanoparticles (NPs) could be profitably exploited...
Abstract High neutrophil to lymphocyte ratio (NLR) and monocyte (MLR) are respectively associated with systemic inflammation immune suppression have been a poor outcome. Plasmatic exosomes extracellular vesicles involved in the intercellular communication system that can exert an immunosuppressive function. Aim of this study was investigate interplay between circulating metastatic breast cancer (MBC). A threshold capable classify patients according MLR, NLR PLR, computed through receiving...
The expression of constitutively active H-RasV12 oncogene has been described to induce proliferative arrest and premature senescence in many cell models. There are a number studies indicating an association between lysosomal enzyme alterations, e.g. β-galactosidase is the most widely used biomarker detect cultured cells we previously reported that up-regulates glycohydrolases enzymatic activity human fibroblasts. Here investigated molecular mechanisms underlying glycohydrolase...
Tuberous sclerosis complex (TSC) is an autosomal dominant genetic disorder caused by mutations in either of two genes, TSC1 or TSC2, resulting the constitutive activation mammalian target rapamycin 1 (mTORC1). mTOR inhibitors are now considered treatment choice for TSC disease. A major pathological feature development subependymal giant cell astrocytomas (SEGAs) brain. Nowadays, it thought that SEGAs could be a consequence aberrant aggregation and migration neural stem/progenitor cells...