A5015725889- Inflammasome and immune disorders
- Molecular Biology Techniques and Applications
- Porphyrin Metabolism and Disorders
- MicroRNA in disease regulation
- RNA modifications and cancer
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Diabetes and associated disorders
- Heavy Metals in Plants
- Glycogen Storage Diseases and Myoclonus
- RNA Interference and Gene Delivery
- Characterization and Applications of Magnetic Nanoparticles
- Child Nutrition and Feeding Issues
- Extracellular vesicles in disease
- Nanoparticles: synthesis and applications
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- Optimal Experimental Design Methods
- Hematological disorders and diagnostics
- Full-Duplex Wireless Communications
- Melanoma and MAPK Pathways
- Phytochemistry and Biological Activities
- Neonatal Health and Biochemistry
- Nanoparticle-Based Drug Delivery
- Bacterial Infections and Vaccines
IRCCS Materno Infantile Burlo Garofolo
2014-2024
University of Trieste
2013-2023
University of Naples Federico II
2023
The term “officinal” derives from the Latin and includes all medicinal, aromatic perfume plant species, which have long been a subject of interest for multiple purposes: health, food, pharmacological, cosmetic so on. In this work, study on six different species medicinal plants, particularly characterized by digestive, choleretic diuretic properties, was carried out: rosemary (Rosmarinus officinalis), sage (Salvia laurel (Laurus nobilis), gentian (Gentiana lutea), dandelion (Taraxacum...
Poly-lactide-co-glycolide (PLGA) is one of the few polymers approved by US Food and Drug Administration as a carrier for drug administration in humans; therefore, it most used materials formulation polymeric nanoparticles (NPs) therapeutic purposes. Because cellular uptake NPs hot topic nanomedicine field, development techniques able to ensure incontrovertible evidence presence cells plays key role gaining understanding their potential. On strength this premise, article aims evaluate...
Glycogenosis type II, or Pompe Disease, is a lysosomal storage disease caused by the deficiency of acid alpha-glucosidase (GAA), leading to glycogen accumulation in muscles. A recombinant human GAA (rhGAA, Myozyme®) currently used for enzyme replacement therapy. Despite its efficacy most patients, some them show diminished response treatment with rapidly progressive clinical deterioration, due immuno-mediated inactivation. To demonstrate that Nanoparticles (NPs) could be profitably exploited...
Periodic fever syndromes (PFS) are an emerging group of autoinflammatory disorders. Clinical overlap exists and multiple genetic analyses may be needed to assist diagnosis. We evaluated the diagnostic value a 5-gene sequencing panel (5GP) in patients with undiagnosed PFS. Simultaneous double strand Sanger MEFV, MVK, TNFRSF1A, NLRP3, NLRP12 genes was performed 42 unexplained features were correlated results. None analyzed displayed causative genotype. However, single or variants uncertain...
Introduction Human polyomaviruses (HPyVs) cause persistent/latent infections in a large fraction of the population. HPyV may severe diseases immunocompromised patients. Malawi polyomavirus (MWPyV) is 10th discovered human (HPyV 10). MWPyV was found stool samples healthy children. So far, few investigations carried out on 10 did not find an association with disease. Methods In this study, to verify putative between and diseases, seroprevalence investigated patients affected by i)...
In clinical practice, patients’ tissues are fixed and paraffin-embedded in order to enable histological diagnosis. Nowadays, those also used for molecular characterization. Formalin is the most fixative worldwide, Bouin’s solution some worldwide institutions. Among targets, micro RNAs (miRNAs), single-stranded non-coding comprised of 18 24 nucleotides, have been demonstrated be resistant fixation paraffin-embedding processes, with consequent possible application practice. present study,...
Cutaneous melanoma (CM) incidence is rising worldwide and the primary cause of death from skin disease in Western world. Personal risk factors linked to environmental ultraviolet radiation (UVR) are well-known etiological contributing its development. Nevertheless, UVR can contribute development CM different patterns varying degrees. The present study aimed at investigating whether altitude residence specific types and/or influence progression. To this aim, 306 formalin-fixed...
Most tissues in clinical practice are formalin-fixed and paraffin-embedded for histological as well molecular analyses. The reproducibility uniformity of analyses is strictly dependent on the quality biomolecules, which highly influenced by pre-analytical processes. In this study, effect different fixatives was compared, including formalin, Bouin's solution, RCL2® TAG-1™ fixatives, stringent application ISO standards mouse liver tissue processing, formalin-free transport grossing a...
Congenital disorder of glycosylation (CDG) type Ia is a multisystem that occurs due to mutations in the phosphomannomutase 2 (PMM2) gene, which encodes for an enzyme involved N‑glycosylation pathway. Mutated PMM2 leads reduced conversion mannose‑6‑P mannose‑1‑P, results low concentration levels guanosine 5'‑diphospho‑D‑mannose (GDP‑Man), nucleotide‑activated sugar essential construction protein oligosaccharide chains. In present study, vitro therapeutic approach was used, based on...
Background Staging of melanoma and follow up after diagnosis aims at predicting risk detecting progression or recurrence early stage, respectively in order to timely start and/or change treatment. Tumor thickness according Breslow, status the sentinel node value lactate dehydrogenase (LDH) are well-established prognostic markers for metastatic risk, but reliable biomarkers identifying candidates who may benefit best from medical treatment still warranted. Liquid biopsy has emerged be a...
RNA isolated from fixed and paraffin-embedded tissues is widely used in biomedical research molecular pathology for diagnosis. In the present study, we have set-up a method based on high performance liquid chromatography (HPLC) to investigate effects of different fixatives RNA. By application presented method, which Nuclease S1 enzymatic digestion extracts followed by HPLC analysis, it possible quantify unmodified nucleotide monophosphates (NMPs) mixture recognize their hydroxymethyl...
Autoantibodies against type I interferon (IFN) are associated with a worse outcome in COVID-19. The measurement of cytokine-neutralizing autoantibodies has been limited, hindering understanding their role clinical practice. We showed that an easy and reliable assay can be reproduced validated to measure the neutralizing potency directed or II IFN. Identifying anti-cytokine might reflect on early treatments for subsequent infections, such as antivirals virus-neutralizing monoclonal antibodies.
Periodic fever is not uncommon in childhood and often ascribed to autoinflammatory conditions; however, it may be present also children with cancer. We here describe the case of a 3-year-old boy acute lymphoblastic leukemia who initially presented 4-month history recurrent, stereotyped episodes localized joint pain, separated by completely symptom-free intervals. These symptoms were interpreted as possible syndrome undifferentiated recurrent until more signs became apparent. Our report...
Autoantibodies against type I interferon (IFN) are associated with a worse outcome in COVID-19. The measurement of cytokine-neutralizing antibodies has been limited, hindering understanding their role clinical practice. We showed that an easy and reliable assay can be reproduced validated to measure the neutralizing potency autoantibodies directed or II IFN. identification anti-cytokine might reflect on early treatments event subsequent infections, such as antivirals monoclonal antibodies.
Erythropoietic protoporphyria is a rare disorder which represents an important health problem in children, causing painful photosensitivity. Little known on the correlation between genetic profile and clinical manifestations. The standard of care for based avoiding sun using protections, but recent literature has suggested that cimetidine may have role improving sensitivity. Herein we report our case series describing successful use analyzing potential phenotype-genotype correlations.This...