A5066132790- Pancreatic function and diabetes
- Diabetes and associated disorders
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Glycosylation and Glycoproteins Research
- Neuroendocrine Tumor Research Advances
- Pancreatic and Hepatic Oncology Research
- Pancreatitis Pathology and Treatment
- Diabetes Management and Research
- Cancer Diagnosis and Treatment
Novo Nordisk (Denmark)
2017
Steno Diabetes Centers
2008
To characterize the EndoC-βH1 cell line as a model for human beta cells and evaluate its functionality, focusing on insulin secretion, proliferation, apoptosis ER stress, with objective to assess potential screening platform identification of novel anti-diabetic drug candidates.EndoC-βH1 was transplanted into mice validation in vivo functionality. Insulin secretion evaluated cultured monolayer pseudoislets, well diabetic mice. Cytokine induced apoptosis, glucolipotoxicity, stress responses...
The regulation of the growth pancreatic β-cell is poorly understood. There are previous indications a role GH in and insulin production islets. In present study we evidence for direct long-term effect on proliferation biosynthesis β-cells monolayer culture. culture medium RPMI 1640 supplemented with 2% normal human serum islets or dissociated islet cells from newborn rats maintained their insulin-producing capacity. When 1–1000 ng/ml pituitary recombinant attached, spread out, proliferated...
The pancreatic beta-cell is a major site of islet amyloid polypeptide (IAPP) biosynthesis, and the peptide coreleased with insulin. We have analyzed expression IAPP (mRNA protein) in various cell types normal transformed murine cultures by Northern blot analyses immunocytochemistry. primarily coexpressed insulin GH-promoted primary rat cultures. Additionally, small population non-beta-cells exhibited prominent expression, double staining experiments showed colocalization glucagon or...