A5059614538- Pancreatic function and diabetes
- Genetics and Neurodevelopmental Disorders
- Protein Degradation and Inhibitors
- Diabetes Treatment and Management
- Diabetes and associated disorders
- Pluripotent Stem Cells Research
- Metabolism, Diabetes, and Cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Virus-based gene therapy research
- Diabetes Management and Research
Novo Nordisk Foundation
2018-2019
University of Copenhagen
2017-2019
Novo Nordisk (Denmark)
2016-2018
Technical University of Denmark
2014
To characterize the EndoC-βH1 cell line as a model for human beta cells and evaluate its functionality, focusing on insulin secretion, proliferation, apoptosis ER stress, with objective to assess potential screening platform identification of novel anti-diabetic drug candidates.EndoC-βH1 was transplanted into mice validation in vivo functionality. Insulin secretion evaluated cultured monolayer pseudoislets, well diabetic mice. Cytokine induced apoptosis, glucolipotoxicity, stress responses...
The production of insulin-producing β cells from human embryonic stem (hESCs) in vitro represents a promising strategy for cell-based therapy type 1 diabetes mellitus. To explore the cellular heterogeneity and temporal progression endocrine progenitors their progeny, we performed single-cell qPCR on more than 500 across several stages differentiation hESCs compared them with islets. We reveal distinct subpopulations along path an early lineage bifurcation toward either polyhormonal or β-like...
To decipher the populations of cells present in human fetal pancreas and their lineage relationships, we developed strategies to isolate pancreatic progenitors, endocrine progenitors cells. Transcriptome analysis individual revealed a large degree conservation among vertebrates drivers gene expression changes occurring at different steps differentiation, although notably, sometimes, members same family are expressed. The transcriptome establishes resource identify novel genes pathways...
A new versatile mammalian vector system for protein production, cell biology analyses, and factory engineering was developed. The applies the ligation-free uracil-excision based technique – USER cloning to rapidly construct expression vectors of multiple DNA fragments with maximum flexibility, both choice backbone cargo. includes a set basic toolbox containing multitude building blocks including promoters, terminators, selectable marker- reporter genes, sequences encoding an internal...
Bromodomain and extraterminal (BET) proteins are epigenetic readers that interact with acetylated lysines of histone tails. Recent studies have demonstrated their role in cancer progression because they recruit key components the transcriptional machinery to modulate gene expression. However, during embryonic development pancreas has never been studied. Using mouse pancreatic explants human induced pluripotent stem cells (hiPSCs), we show BET protein inhibition I-BET151 or JQ1 enhances...