A5044711649- Pancreatic function and diabetes
- Diabetes and associated disorders
- Genetics and Neurodevelopmental Disorders
- Congenital heart defects research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
- Neurobiology and Insect Physiology Research
- Plant Molecular Biology Research
- Regulation of Appetite and Obesity
- Physiological and biochemical adaptations
- Kruppel-like factors research
- Colorectal Cancer Surgical Treatments
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Receptor Mechanisms and Signaling
- Pluripotent Stem Cells Research
- Genetic Syndromes and Imprinting
- Pancreatitis Pathology and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Colorectal Cancer Screening and Detection
- Fibroblast Growth Factor Research
Novo Nordisk (Denmark)
2013-2024
Novo Nordisk (United Kingdom)
2023
Rigshospitalet
2019-2022
Copenhagen University Hospital
2021-2022
Molecular Biology Consortium
2011
Dorn Research Institute
2010
University of Copenhagen
2009
Steno Diabetes Centers
2003-2007
To characterize the EndoC-βH1 cell line as a model for human beta cells and evaluate its functionality, focusing on insulin secretion, proliferation, apoptosis ER stress, with objective to assess potential screening platform identification of novel anti-diabetic drug candidates.EndoC-βH1 was transplanted into mice validation in vivo functionality. Insulin secretion evaluated cultured monolayer pseudoislets, well diabetic mice. Cytokine induced apoptosis, glucolipotoxicity, stress responses...
<h3>Objective</h3> Growing evidence suggests that a phenotypic switch converting pancreatic acinar cells to duct-like can lead intraepithelial neoplasia and eventually invasive ductal adenocarcinoma. Histologically, the onset of this is characterised by co-expression markers in acini, lesion called acinar-to-ductal metaplasia (ADM). The transcriptional regulators required initiate ADM are unknown, but need be identified characterise regulatory networks drive ADM. In study, role transcription...
G protein-coupled receptor (GPR)-39 is a seven-transmembrane expressed mainly in endocrine and metabolic tissues that acts as Zn++ sensor signaling through the Gq G12/13 pathways. The expression of GPR39 regulated by hepatocyte nuclear factor (HNF)-1α HNF-4α, present study, we addressed importance for glucose homeostasis pancreatic islets function. localization were characterized pancreas cell lines. Gpr39(−/−) mice studied vivo, especially respect tolerance insulin sensitivity, vitro islet...
Mice genetically deficient in the glucagon receptor (Gcgr−/−) show improved glucose tolerance, insulin sensitivity, and α-cell hyperplasia. In addition, Gcgr−/− mice do not develop diabetes after chemical destruction of β-cells. Since fibroblast growth factor 21 (FGF21) has insulin-independent glucose-lowering properties, we investigated whether FGF21 was contributing to resistance insulin-deficient mice. Plasma 25-fold higher than wild-type found be expressed pancreatic β- α-cells, with...
We have developed a wholemount immunofluorescence protocol for the simultaneous detection of up to three proteins in mouse and chicken embryos. Combined with Murray's clearing reagent (BABB) microscope objectives long working ranges high numerical apertures mounted on confocal microscope, cellular resolution can be obtained depths offering possibility examining expression patterns entire organs or Three-dimensional projections optical sections computed computer software allowing rotation...
Type 1 diabetes (T1D) is a complex disease characterized by the loss of insulin-secreting β-cells. Although has strong genetic component, and several loci are known to increase T1D susceptibility risk, only few causal genes have currently been identified. To identify disease-causing in T1D, we performed an silico “phenome–interactome analysis” on genome-wide linkage scan dataset. This method prioritizes candidates according their physical interactions at protein level with other proteins...
Ptf1a and Pdx1 are critical transcription factors of early pancreatic development, as shown by loss function studies where lack each gene alone causes almost complete pancreas agenesis. is particularly interesting because it linked to a recently reported signature expression profile associated with the multipotent condition. Few useful antibody reagents have been available for consistent reliable immunohistochemical visualization protein in developing which level production this regulator...
Glioblastoma (GBM) remains one of the most lethal brain tumors, with standard-of-care therapies such as radiation and temozolomide offering limited survival benefit. Patient-derived xenograft (PDX) models have become critical tools for studying treatment resistance identifying novel therapeutic strategies. Here, we report establishment characterization a GBM cell line, ST146c, derived from radiation-resistant PDX model (ST146). This line retains therapy-resistant phenotype observed in...
Abstract Introduction: Targeted radionuclide therapy (TRT) is a unique treatment option allowing for in vivo imaging and delivery of lethal radiation directly specifically to solid cancers. As TRT generally well-tolerated with limited side effects, it holds significant potential use combination other therapies such as immunotherapy. However, preclinical assessment this combinational pair has been by the lack relevant animal models. To address this, we established fully immunocompetent model...
Abstract Obesity impairs tissue insulin sensitivity and signaling, promoting type-2 diabetes. Although improving signaling is key to reversing diabetes, the multi-organ mechanisms regulating this process are poorly defined. Here, we screen secretome receptome in Drosophila identify hormonal crosstalk affecting diet-induced resistance obesity. We discover a complex interplay between muscle, neuronal, adipose tissues, mediated by Bone Morphogenetic Protein (BMP) hormone Bursicon, that enhances...
Genetic studies have shown that formation of pancreatic endocrine cells in mice is dependent on the cell autonomous action bHLH transcription factor Neurogenin3 and extent timing differentiation controlled by Notch signaling. To further understand mechanism which exerts this function, we investigated development chicken embryos.In situ hybridization showed expression signaling components pro-endocrine factors conserved to a large degree between mouse. Cell inhibition signal reception results...
The aim of this study was to characterize two monoclonal antibodies (F6A11 and F109-D12) generated against Pdx1 (pancreatic duodenal homeobox-1), a homeodomain transcription factor, which is critical for pancreas formation as well normal pancreatic beta cell function. For production antibodies, we immunized Robertsonian POSF (RBF)mice with GST-Pdx1 fusion protein containing 68-amino acid C-terminal fragment rat Pdx1. These detect by western blotting allow immunohistochemical detection in...
HLA-DRB1*08:112 differs from HLA-DRB1*08:01 in exon 2 at amino acid 62; asparagine to lysine substitution.
Recurrent extraluminal rectal carcinoma: transrectal biopsy under sonographic guidance.M B Nielsen, J F Pedersen, Hald and ChristiansenAudio Available | Share
HLA‐C*07:780 differs from HLA‐C*07:04:01:01 in exon 2 at amino acid 49; alanine to threonine substitution.
HLA‐DPA1*01:46 differs from HLA‐DPA1*01:03 in exon 2 at amino acid 85; Aspartate to Asparagine substitution.
HLA-DQA1*01:65 differs from HLA-DQA1*01:03 in exon 1 at amino acid -7 a valine to methionine substitution.
Abstract Obesity leads to impaired insulin signaling and tissue sensitivity, which drive the onset of type 2 diabetes. Insulin resistance a reduction in cellular glucose uptake, resulting elevated blood levels, consequently cause β-cell dysfunction development Although improving is key target for restoring whole-body homeostasis reversing diabetes, multi-organ mechanisms that regulate sensitivity are poorly defined. We screened secretome receptome Drosophila identify underlying interorgan...
<title>Abstract</title> Obesity leads to impaired insulin signaling and tissue sensitivity, which drive the onset of type 2 diabetes. Insulin resistance a reduction in cellular glucose uptake, resulting elevated blood levels, consequently cause β-cell dysfunction development Although improving is key target for restoring whole-body homeostasis reversing diabetes, multi-organ mechanisms that regulate sensitivity are poorly defined. We screened secretome receptome Drosophila identify...