A5064950632- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Endometriosis Research and Treatment
- Molecular Biology Techniques and Applications
- Genetic and phenotypic traits in livestock
- Birth, Development, and Health
- Reproductive Biology and Fertility
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Pregnancy and preeclampsia studies
- S100 Proteins and Annexins
- Selenium in Biological Systems
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Sperm and Testicular Function
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Blood Coagulation and Thrombosis Mechanisms
- Animal Genetics and Reproduction
- Dietary Effects on Health
- Apelin-related biomedical research
- Medicinal Plants and Bioactive Compounds
- Trace Elements in Health
- Immunotoxicology and immune responses
University of Hong Kong
2022-2025
University of Hong Kong - Shenzhen Hospital
2022-2025
Huazhong Agricultural University
2018-2023
Shenzhen Institutes of Advanced Technology
2022-2023
Chinese Academy of Sciences
2022
Shenzhen University
2022
ABSTRACT The decline of oocyte quality with advanced maternal age has a detrimental effect on female fertility. However, there is limited knowledge therapeutic options and their mechanisms to improve in reproductively older women. In this study, we demonstrated that supplementation salidroside improves the old mice. Salidroside improved maturation, fertilization, developmental competence oocytes from mice by maintaining normal spindle/chromosome structure mitochondrial function. Oocyte...
Endometrial receptivity plays a vital role in successful embryo implantation pigs. MicroRNAs (miRNAs), known as regulators of gene expression, have been implicated the regulation implantation. However, miRNAs endometrial during pre-implantation period remains elusive. In this study, we report that expression level Sus scrofa (ssc)-miR-21-5p porcine endometrium tissues was significantly increased from day 9 to 12 pregnancy. Knockdown ssc-miR-21-5p inhibited proliferation and migration...
Chemotherapy is extensively used to treat cancers and often associated with ovarian damage leads premature insufficiency infertility, while the role of mitochondria during chemotherapy remains unknown. This study a mouse model oocyte-specific deletion mitochondrial stress response gene Caseinolytic peptidase P ( Clpp ) investigate homeostasis in oocytes from mice receiving chemotherapeutic drug cyclophosphamide (CTX). We found that reduced fecundity at advanced age. The led meiotic defects...
Embryonic implantation involves a complex and well‐coordinated interaction between the developing conceptus maternal uterus, preimplantation period has major impact on litter size in pigs. The present study aimed to investigate vital messenger RNAs (mRNAs) long noncoding (lncRNAs) that regulate Meishan enriched Gene Ontology terms were all related “binding.” Furthermore, “ECM‐receptor interaction” was predicted as an important pathway regulated success of implantation. We speculated...
Heat stress (HS) poses a significant threat to production and survival in the global swine industry. However, molecular regulatory effects of heat on maternal endometrial cells are poorly understood pigs during early embryo implantation. In this study, we systematically examined morphological changes endometrium corresponding regulation mechanism response HS by combining scanning electron microscopy (SEM), hematoxylin/eosin (H&E) staining, western blot, RNA-seq analyses. Our results...
Objective The aim of this study was to explore the symptoms, treatment, and pathogenesis ovarian juvenile granulosa cell tumors with Ollier’s disease in children. Methods From October 2019 2020, clinical data were retrospectively analyzed for one case disease. Whole-exome sequencing Sanger used detect gene mutation tumor chondroma tissue. NADP-dependent isocitrate dehydrogenase-1 (IDH1) S6 ribosomal protein expression levels cells transfected wild-type or mutant plasmid by Western blot....
Caseinolytic protease proteolytic subunit (ClpP) and caseinolytic X (ClpX) are mitochondrial matrix peptidases that activate unfolded protein response to maintain homeostasis in the mitochondria. However, role of ClpP ClpX spermatogenesis remains largely unknown. In this study, we demonstrated importance ClpP/ClpX for meiosis with two conditional knockout (cKO) mouse models. We found deficiency reduced functions quantity spermatocytes, affected energy supply during attenuated...
Contents Annexin A8 ( ANXA8 ) gene, a member of the annexin family, encodes an anticoagulant protein involved in blood coagulation cascade and acts as indirect inhibitor thromboplastin‐specific complex. However, little is known about function porcine endometrial cells so far. Here, mRNA was found to be abundant endometrium on days 11–13 pregnancy. Real‐time RT–PCR analysis indicated that expression leukaemia inhibitory factor LIF epidermal growth EGF upregulated by cells. Immunofluorescence...
Mitochondria are highly dynamic organelles and their activity is known to be regulated by changes in morphology via fusion fission events. However, the role of mitochondrial dynamics on cellular differentiation remains largely unknown. Here, we explored molecular mechanism during spermatogenesis generating an Mfn2 (mitofusin 2) conditional knock-out (cKO) mouse model. We found that depletion MFN2 male germ cells led disrupted meiosis which majority cKO spermatocytes did not develop pachytene...
Abstract Background Endometrial receptivity plays a vital role in the success of embryo implantation. However, temporal proteomic profile porcine endometrium during implantation is still unclear. Results In this study, abundance proteins on days 9, 10, 11, 12, 13, 14, 15 and 18 pregnancy (D9, 18) was profiled via iTRAQ technology. The results showed that 25, 55, 103, 91, 100, 120, 149 were up-regulated, 24, 70, 169, 159, 164, 161, 198 down-regulated D10, compared with D9, respectively. Among...
Extracellular vesicles (EVs) could mediate embryo-maternal communication to affect embryo implantation by delivering biology information, including microRNA (miRNA), protein, lipid. Our previous research shows that miR-92b-3p was differentially expressed in EVs of uterine flushing fluids during the period. However, role from remains elusive.EVs were isolated porcine endometrial epithelial cells (EECs) ultracentrifugation. MiR-92b-3p mimics and used regulate expression trophoblast (PTr2...
Abstract Background The exosomes of uterine luminal fluid (ULF) mediate intrauterine communication between conceptus and uterus in pigs. small RNAs ULF are widely recognized as important factors that influence embryonic implantation humans, mice, cattle sheep. Therefore, it can be hypothesized essential for Results Cup-shaped (diameters: 40–160 nm) porcine were isolated characterized, then PKH67 stained taken up by endometrial epithelial cells trophectoderm cells. expression these on days 10...
Abstract Background: The extracellular vesicles (EVs) of uterine flushing fluids (UFs) mediate intrauterine communication between conceptus and uterus in pigs. small RNAs UFs-EVs are widely recognized as important factors that influence embryonic implantation humans, mice, cattle sheep. Therefore, it can be hypothesized essential for Results: Cup-shaped EVs porcine UFs were isolated characterized, then PKH67 stained taken up by endometrial epithelial cells trophectoderm a time-dependent...
Abstract Background The exosomes of uterine luminal fluid (ULF) mediate intrauterine communication between conceptus and uterus in pigs. small RNAs ULF are widely recognized as important factors that influence embryonic implantation humans, mice, cattle sheep. Therefore, it can be hypothesized essential for Results Cup-shaped (diameters: 40–160 nm) porcine were isolated characterized, then PKH67 stained taken up by endometrial epithelial cells trophectoderm cells. expression these on days 10...
Abstract Caseinolytic protease proteolytic subunit (ClpP) and caseinolytic X (ClpX) are mitochondrial matrix peptidases that activate unfolded protein response to maintain homeostasis in the mitochondria. However, role of ClpP ClpX spermatogenesis remains largely unknown. In this study, we demonstrated importance ClpP/ClpX for meiosis with two conditional knockout (cKO) mouse models. We found deficiency reduced functions quantity spermatocytes, affected energy supply during attenuated...