A5014214674- Reproductive Biology and Fertility
- Ovarian function and disorders
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- Tissue Engineering and Regenerative Medicine
- Assisted Reproductive Technology and Twin Pregnancy
- Sperm and Testicular Function
- Birth, Development, and Health
- Generative Adversarial Networks and Image Synthesis
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Face recognition and analysis
- Reproductive System and Pregnancy
- Reproductive Health and Technologies
- Renal and related cancers
- Genetic Syndromes and Imprinting
- Endometriosis Research and Treatment
- Ovarian cancer diagnosis and treatment
- Mesenchymal stem cell research
- Advanced Image Processing Techniques
- Selenium in Biological Systems
- Gynecological conditions and treatments
- Neuroblastoma Research and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Bioenergy crop production and management
University of Hong Kong - Shenzhen Hospital
2021-2025
University of Hong Kong
2021-2025
Capital Medical University
2024-2025
First Affiliated Hospital of Xi'an Jiaotong University
2022-2024
Soochow University
2024
First Affiliated Hospital of Soochow University
2024
Changhai Hospital
2024
Qingdao University
2024
Jilin Jianzhu University
2024
Second Military Medical University
2024
Abstract Aneuploidy, the presence of an abnormal number chromosomes, is a major cause early pregnancy loss in humans. Yet, developmental consequences specific aneuploidies remain unexplored. Here, we determine extent post-implantation development human embryos bearing common using recently established culture platform. We show that while trisomy 15 and 21 develop similarly to euploid embryos, monosomy exhibit high rates arrest, 16 display hypo-proliferation trophoblast, tissue forms...
Abstract Mitochondria are dynamic organelles that continually adapt their structure through fusion and fission in response to changes bioenergetic environment. Targeted deletion of mitochondrial protein mitofusin1 (MFN1) oocytes resulted female infertility associated with failure achieve oocyte maturation. Oocyte-granulosa cell communication was impaired, cadherins connexins were downregulated, resulting follicle developmental arrest at the secondary stage. Deletion MFN1 dysfunction altered...
Summary Caseinolytic peptidase P mediates degradation of unfolded mitochondrial proteins and activates protein response (mt UPR ) to maintain homeostasis. Clpp −/− female mice generate a lower number mature oocytes two‐cell embryos, no blastocysts. have smaller mitochondria, with aspect ratio (length/width), decreased expression genes that promote fusion. A 4‐fold increase in atretic follicles at 3 months, reduced primordial 6–12 months are observed ovaries. This is associated upregulation...
Mitochondria change their shape through fusion and fission in order to adapt metabolic milieu. Mitofusin-2 (MFN2) is a key regulatory protein this process, mediating mitochondrial interaction with endoplasmic reticulum. Targeted deletion of Mfn2 oocytes resulted dysfunction female subfertility associated impaired oocyte maturation follicle development. Oocytes lacking MFN2 showed shortened telomeres increased apoptosis, resulting compromised quality accelerated follicular depletion,...
Maternal effect genes play essential roles in early embryonic development. However, the mechanisms by which maternal regulate mammalian development remain largely unknown. Recently, we identified a subcortical complex (SCMC) that is composed of at least four proteins encoded Mater, Floped, Tle6 and Filia critical for mouse preimplantation The present study demonstrates human SCMC homologous (NLRP5, OOEP, TLE6 KHDC3L) are specifically expressed oocytes fetal ovaries. this co-localize...
The importance of autophagy in polycystic ovary syndrome (PCOS)-related metabolic disorders is increasingly being recognized, but few studies have investigated the role PCOS. Here, transmission electron microscopy demonstrated that was enhanced ovarian tissue from both humans and rats with Consistent this, granulosa cells PCOS showed increases marker protein light chain 3B (LC3B), whereas levels substrate SQSTM1/p62 were decreased. In addition, ratio LC3-II/LC3-I markedly elevated human...
Do long non-coding RNA (lncRNA) and messenger (mRNA) profiles in follicular fluid from mature immature ovarian follicles differ between healthy women with polycystic ovary syndrome (PCOS)? lncRNA mRNA both differed significantly PCOS patients. Unlike microRNAs, which have been extensively studied, lncRNAs present never sequenced the biological associations of who develop remain largely unknown. A total 18 subjects (8 controls 10 patients) were recruited to participate this study. Recruitment...
Weaver syndrome (WS), an overgrowth/intellectual disability (OGID), is caused by pathogenic variants in the histone methyltransferase EZH2, which encodes a core component of Polycomb repressive complex-2 (PRC2). Using genome-wide DNA methylation (DNAm) data for 187 individuals with OGID and 969 control subjects, we show that EZH2 generate highly specific sensitive DNAm signature reflecting phenotype WS. This can be used to distinguish loss-of-function from gain-of-function missense detect...
Transplantation of cryopreserved ovarian tissue is a novel technique to restore endocrine function and fertility especially for cancer patients. However, the main obstacle massive follicle loss as result ischemia in process transplantation. Mesenchymal stem cells (MSCs) have been acknowledged play an important role supporting angiogenesis stabilizing long-lasting blood vessel networks through release angiogenic factors differentiation into pericytes endothelial cells. This study aimed...
What is the effect of human ovarian tissue cryopreservation on single follicular development in vitro? Vitrification had a greater negative growth and gene expression follicles when compared with fresh follicles. For cortex cryopreservation, conventional option slow freezing while more recently vitrification has been demonstrated to maintain good quality function tissues. Ovarian tissues were collected from 11 patients. every patient, was divided into three samples: Fresh, slow-rate (Slow)...
We have recently reported that human bone marrow-derived mesenchymal stem cells (MSCs) facilitate angiogenesis and prevent follicle loss in xenografted ovarian tissues. However, the mechanism underlying this effect remains to be elucidated. Thus, determining paracrine profiles identifying key secreted factors MSCs co-transplanted with grafts are essential for future application of MSCs.In study, we used cytokine microarrays identify differentially expressed proteins associated frozen-thawed...
Semen samples from men after a short ejaculatory abstinence show improved sperm quality and result in increased pregnancy rates, but the underlying mechanisms remain unclear. Herein, we report that ejaculates (1–3 h) compared with long (3–7 days) periods of showed increases motile count, vitality, normal morphology, acrosome reaction capacity, total antioxidant mitochondrial membrane potential, high DNA stainability, decrease fragmentation index (p, < 0.05). Sperm proteomic analysis 322...
Text-to-Face (TTF) synthesis is a challenging task with great potential for diverse computer vision applications. Compared to Text-to-Image (TTI) tasks, the textual description of faces can be much more complicated and detailed due variety facial attributes parsing high dimensional abstract natural language. In this paper, we propose model that not only produces images in resolution (1024×1024) text-to-image consistency, but also outputs multiple cover wide range unspecified features way. By...
Does basic fibroblast growth factor (bFGF) in combination with fibrin hydrogel improve follicle development and revascularization of heterotopically transplanted mouse ovarian tissues? Treatment tissues higher concentrations (75, 100 150 μg/ml), but not lower (25 50 bFGF significantly improved primordial survival angiogenesis, while apoptosis follicles stromal cells was decreased. Use female fertility preservation is limited by the massive loss ischemia-reperfusion injury due to expected...
What is the effect of basic fibroblast growth factor (bFGF) on individual early human follicles in a three-dimensional (3D) culture system vitro? The addition 200 ng bFGF/ml improves follicle growth, survival and viability during vitro. It has been demonstrated that bFGF enhances primordial development ovarian tissue culture. However, encapsulated 3D have not reported. maturation vitro was investigated. Ovarian (n= 11) obtained from 11 women laparoscopic surgery for gynecological disease,...
Using glass sand as a substitute for in high-performance concrete not only conserves resources but also helps protect the environment. This study replaced manufactured with and added carbon fiber to prepare shotcrete. Its mechanical self-sensing properties were then tested separately. The findings suggest that an increase content, compressive strength of shotcrete initially rises subsequently declines. When replacement ratio is 75%, exhibits its maximum value, whereas content leads reduction...
ABSTRACT The decline of oocyte quality with advanced maternal age has a detrimental effect on female fertility. However, there is limited knowledge therapeutic options and their mechanisms to improve in reproductively older women. In this study, we demonstrated that supplementation salidroside improves the old mice. Salidroside improved maturation, fertilization, developmental competence oocytes from mice by maintaining normal spindle/chromosome structure mitochondrial function. Oocyte...