A5115603847- Reproductive Biology and Fertility
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- Renal and related cancers
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Reproductive Health and Technologies
- Reproductive System and Pregnancy
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Assisted Reproductive Technology and Twin Pregnancy
- Tissue Engineering and Regenerative Medicine
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA modifications and cancer
- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- Genetic Syndromes and Imprinting
- Sexual Differentiation and Disorders
- COVID-19 Impact on Reproduction
- Bioenergy crop production and management
- Thyroid Cancer Diagnosis and Treatment
- Supramolecular Self-Assembly in Materials
- interferon and immune responses
Peking University Third Hospital
2014-2024
Peking University
2014-2024
Beijing Obstetrics and Gynecology Hospital
2013-2022
Ministry of Education of the People's Republic of China
2012-2022
National Clinical Research Center for Digestive Diseases
2020-2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2022
Shandong University
2022
Beijing Advanced Sciences and Innovation Center
2020
Imaging Center
2015
Nanjing Normal University
2014
The cellular complexity of human brain development has been intensively investigated, although a regional characterization the entire cerebral cortex based on single-cell transcriptome analysis not reported. Here, we performed RNA-seq over 4,000 individual cells from 22 regions mid-gestation embryos. We identified 29 cell sub-clusters, which showed different proportions in each region and pons especially high percentage astrocytes. Embryonic neurons were as diverse adult neurons, they...
PolyA– RNAs have not been widely analyzed in human pre-implantation embryos due to the scarcity of materials. In particular, circular RNA (circRNA), a novel type polyA– RNA, has characterized during development. We systematically analyze polyA+ messenger (mRNAs) and individual oocytes using SUPeR-seq. de novo identify 10,032 circRNAs from 2974 hosting genes. Most these are developmentally stage-specific dynamically regulated. Many them maternally expressed, implying their potentially...
Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, comprehensive chromatin state has not yet been analyzed fetal cells (FGCs). Here we use nucleosome occupancy and methylation sequencing method to analyze both genome-wide accessibility DNA methylome at a series crucial time points during cell development in mouse. We find 116 887 137 557 nucleosome-depleted regions (NDRs) mouse FGCs, covering large set germline-specific highly dynamic...
Various types of cells are routinely cryopreserved in modern regenerative and cell-based medicines. For instance, the oocyte is one most demanding to be genetic engineering human-assisted reproductive technology (ART). However, usage oocytes ART clinics still limited mainly because unstable survival rate. This due fact that more prone damaged by ice crystals comparison other cells, as larger size surface area. Meanwhile, contain water, thus, easier form inside cells. Currently, avoid injury...
Mechanisms of implantation such as determination the attachment pole, fetal-maternal communication, and underlying causes failure are largely unexplored. Here, we performed single-cell RNA sequencing on peri-implantation embryos from both humans mice to explore trophectoderm (TE) development embryo-endometrium cross-talk. We found that transcriptomes polar mural TE diverged after hatched zona pellucida in species, with being more mature than TE. The poles show similarities cell cycle...
Does Sirt3 dysfunction result in poor developmental outcomes for human oocytes after vitro maturation (IVM)?Inefficient expression induced decreased mitochondrial DNA copy number and biogenesis, therefore impaired the competence of IVM oocytes.Cytoplasmic immaturity may lead to reduced competence. Mitochondrial results accumulation free radicals leads mutations, protein damage, telomere shortening apoptosis. SIRT3 (in Sirtuin family) has emerged as a fidelity that directs energy generation...
Feathers are hallmark avian integument appendages, although they were also present on theropods. They composed of flexible corneous materials made α- and β-keratins, but their genomic organization functional roles in feathers have not been well studied. First, we an exhaustive search β-keratin genes the new chicken genome assembly (Galgal4). Then, using transcriptomic analysis, studied gene expression patterns five types feather epidermis. The different types, whereas those α-keratin less...
Transplantation of cryopreserved ovarian tissue is a novel technique to restore endocrine function and fertility especially for cancer patients. However, the main obstacle massive follicle loss as result ischemia in process transplantation. Mesenchymal stem cells (MSCs) have been acknowledged play an important role supporting angiogenesis stabilizing long-lasting blood vessel networks through release angiogenic factors differentiation into pericytes endothelial cells. This study aimed...
Abstract Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as caused by freezing thawing in many species including plant, ram sperm human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied mammalian oocyte vitrification. In study we evaluated the efficiency safety of new The results indicated improves survival rate vitrified oocytes, protects mitochondrial functions could be mouse
DNA methylation plays important roles during development. However, the reprogramming of functional elements has not been fully investigated mammalian embryonic Herein, using our modified MethylC-Seq library generation method and published post-bisulphite adapter-tagging (PBAT) method, we generated genome-wide methylomes human gametes early embryos at single-base resolution compared them with mouse methylomes. We showed that dynamics in are conserved between humans mice embryogenesis, except...
What is the effect of human ovarian tissue cryopreservation on single follicular development in vitro? Vitrification had a greater negative growth and gene expression follicles when compared with fresh follicles. For cortex cryopreservation, conventional option slow freezing while more recently vitrification has been demonstrated to maintain good quality function tissues. Ovarian tissues were collected from 11 patients. every patient, was divided into three samples: Fresh, slow-rate (Slow)...
We have recently reported that human bone marrow-derived mesenchymal stem cells (MSCs) facilitate angiogenesis and prevent follicle loss in xenografted ovarian tissues. However, the mechanism underlying this effect remains to be elucidated. Thus, determining paracrine profiles identifying key secreted factors MSCs co-transplanted with grafts are essential for future application of MSCs.In study, we used cytokine microarrays identify differentially expressed proteins associated frozen-thawed...
Cryosurgery has attracted much attention for the treatment of tumors owing to its clear advantages. However, determining volume frozen tissues in real-time remains a challenge, which greatly lowers therapeutic efficacy cryosurgery and hinders broad application cancers. Herein, we report freezing-induced turn-on strategy selective imaging cancer cells. As type aggregation-induced emission (AIE) fluorogen, TABD-Py molecules interact specifically with ice crystals form aggregates at ice/water...
Objective One of the major obstacles to ovarian tissue preservation is delayed angiogenesis that leads follicles lost after transplantation. The aim present study was investigate effects bFGF and VEGF on heterotopic transplanted using a mouse model. Methods Female mice underwent bilateral ovariectomy. Ovarian tissues encapsulated by fibrin hydrogels were subcutaneously into recipient mice, in which hormonal cyclicity absent. fibrinogen solution mixed with bFGF, VEGF, or mixture VEGF. grafts...