A5100361079- Renal and related cancers
- Renal cell carcinoma treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Prenatal Screening and Diagnostics
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Chronic Kidney Disease and Diabetes
- Cancer-related molecular mechanisms research
- Reproductive biology and impacts on aquatic species
- Urological Disorders and Treatments
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Osteoarthritis Treatment and Mechanisms
- Genetic Syndromes and Imprinting
- Cardiac Arrest and Resuscitation
- Sexual Differentiation and Disorders
- Immune cells in cancer
- Cleft Lip and Palate Research
- Craniofacial Disorders and Treatments
- Congenital heart defects research
- Childhood Cancer Survivors' Quality of Life
- Trauma and Emergency Care Studies
- Genetic and Kidney Cyst Diseases
Westlake University
2025
Zunyi Medical University
2024
Kunming Children's Hospital
2019-2023
Boston Children's Hospital
2022-2023
Harvard University
2023
Peking University
2017-2022
Shanghai Jiao Tong University
2022
State Key Laboratory of Oncogene and Related Genes
2022
Kunming Medical University
2021
Hangzhou First People's Hospital
2021
Understanding the molecular mechanisms underlying human cartilage degeneration and regeneration is helpful for improving therapeutic strategies treating osteoarthritis (OA). Here, we report programmes lineage progression patterns controlling OA pathogenesis using single-cell RNA sequencing (scRNA-seq).We performed unbiased transcriptome-wide scRNA-seq analysis, computational analysis histological assays on 1464 chondrocytes from 10 patients with undergoing knee arthroplasty surgery. We...
Patients with 46,XY gonadal dysgenesis (GD) exhibit genital anomalies, which range from hypospadias to complete male-to-female sex reversal. However, a molecular diagnosis is made in only 30% of cases. Heterozygous mutations the human FGFR2 gene cause various craniosynostosis syndromes including Crouzon and Pfeiffer, but testicular defects were not reported. Here, we describe patient whose features would suggest represent new FGFR2-related syndrome, XY reversal or CSR. The was chromosomally...
Abstract Proper development of fetal germ cells (FGCs) is vital for the precise transmission genetic and epigenetic information through generations. The transcriptional landscapes human FGC have been revealed; however, reprogramming process FGCs remains elusive. Here, we profiled genome-wide DNA methylation chromatin accessibility at different phases as well gonadal niche single-cell resolution. First, found that levels changed in a temporal manner, whereas same embryo exhibited comparable...
Gonadal somatic cells are the main players in gonad development and important for sex determination germ cell development. Here, using a time-series single-cell RNA sequencing (scRNA-seq) strategy, we analyzed fetal (FGCs) gonadal human embryos fetuses. Clustering analysis of testes ovaries revealed several novel subsets, including POU5F1+SPARC+ FGCs KRT19+ cells. Furthermore, our data indicated that bone morphogenetic protein (BMP) signaling pathway plays type-specific developmental...
Summary Cellular lineage histories along with their molecular states encode fundamental principles of tissue development and homeostasis. Current lineage-recording mouse models have limited barcode diversity poor single-cell coverage, thus precluding use in tissues composed millions cells. Here, we developed DARLIN, an improved Cas9 barcoding line that utilizes terminal deoxynucleotidyl transferase (TdT) to enhance insertion events over 30 CRISPR target sites, stably integrated into 3...
Wilms' tumor (WT) is a major type of kidney cancer in children; however, the therapeutic measures for control metastasis, recurrence and death this remain unsatisfactory. The present study aimed to verify expression T‑cell factor 3 (TCF3) WT, explore its role regulating viability, migration apoptosis cells. Tumor tissues were collected from 10 patients with adjacent as normal controls. levels TCF3 detected WT by reverse transcription‑quantitative PCR (RT‑qPCR), western blotting...
Wilms tumor (WT) is the most prevalent urologic malignancy in childhood.Nonetheless, genetic factors underlying WT remain largely unknown.The miR-423 rs6505162 C>A polymorphism associated with susceptibility to numerous cancers; however, no investigations have been conducted on its association WT.To evaluate correlation between and risk Chinese children, we genotyped this using Taqman method 145 cases 531 cancer-free controls.Odds ratios (ORs) 95% confidence intervals (CIs) were calculated...
Epithelial‑mesenchymal transition (EMT) is considered to be a crucial stage of renal fibrosis. Previous studies have indicated that paired box 2 (PAX2) affects fibrosis, likely by regulating EMT. Therefore, we examined whether PAX2 directly induces normal tubular EMT in vitro. Recombinant plasmid was transfected into epithelial cell line (NRK52E) derived from rats. The optimal time‑point evaluated through fluorescence detection, western blotting and real-time polymerase chain reaction (PCR)....
Objective: To investigate the effect of paired box 2 (PAX2) gene re-expression in renal tubular epithelial cells on interstitial fibrosis rats with obstructive nephropathy. Methods: Wistar were randomly divided into two groups: sham-operated group (sham group) and unilateral ureteral obstruction (UUO group), 40 each group. After surgery, killed at 3, 5, 7, 14 days (n = 10 each) to remove collect kidneys. Morphological changes kidney determined by histopathology. Protein mRNA expression PAX2...
Objective: It was found that PAX2 (Paired Box 2) re-expression in renal tubular epithelial cells correlated with interstitial fibrosis of rats obstructive nephropathy. The purpose the present study to identify whether RNA interference (RNAi) induced by polyethylenimine (jetPEI) could inhibit gene and impact Methods: Four pairs small interfering (siRNA) sequences a negative control were designed synthesized according whole mRNA sequence. siRNA then transfected into capsule rodent model...
Wilms tumor is the most common type of renal malignancy in children. Previous studies have demonstrated that single nucleotide polymorphisms (SNPs) AURKA gene could predispose to several human malignancies. We recruited 145 cases and 531 cancer-free controls investigate whether variants modify susceptibility. Three SNPs (rs1047972 C>T, rs2273535 T>A, rs8173 G>C) were genotyped by Taqman methodology. Odds ratios (ORs) 95% confidence intervals (CIs) used assess strength association between...
Background Malignant rhabdoid tumor of the kidney (MRTK) is a rare type that lacks typical clinical manifestations. Herein, we presented data 2 children with MRTK. In addition, used high-throughput RNA-sequencing (RNA-seq), GO analysis, and KEGG signaling pathway analysis to examine gene expression differences at transcripts level between patients MRTK 3 non-tumor diseases without other symptoms. Case report Preoperative B-scan ultrasonography computed tomography (CT) examination in cases...
YTHDF2 is responsible for maintaining the dynamic N 6 -methyladenosine (m A) modification balance and influences a variety of cancers.We tested whether gene rs3738067 A>G polymorphism related to Wilms tumor by genotyping samples Chinese children (450 cases 1317 controls).However, showed no statistical significance with susceptibility.Stratification analysis also revealed that there was remarkable association variant AG/GG genotype risk in every subgroup (age, gender, clinical stages).In all,...
Papillary renal cell carcinoma (PRCC) is the 2nd most common type of carcinoma; however, there limited data about PRCC, and strategies for diagnosis treatment PRCC need to be identified.In this study, stemness-associated senescence (SAS) phenotype was obtained by a bioinformatics analysis. We acquired gene expression profiles patients with calculated messenger ribonucleic acid stemness index (mRNAsi). then screened SAS genes from GenAge database. A least absolute shrinkage selection...