A5100689059- Gene expression and cancer classification
- Sarcoma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Genomic variations and chromosomal abnormalities
- Gene Regulatory Network Analysis
- Epigenetics and DNA Methylation
- Genomics, phytochemicals, and oxidative stress
- Single-cell and spatial transcriptomics
- Radiomics and Machine Learning in Medical Imaging
- Esophageal Cancer Research and Treatment
- Genetic factors in colorectal cancer
- Adrenal and Paraganglionic Tumors
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer, Hypoxia, and Metabolism
- Prostate Cancer Treatment and Research
- Hormonal Regulation and Hypertension
- Renal and related cancers
- Cell Image Analysis Techniques
- Protein Degradation and Inhibitors
- Cancer-related molecular mechanisms research
- Prenatal Screening and Diagnostics
- RNA modifications and cancer
Peking University
2012-2024
Peking University Third Hospital
2020-2024
Beijing Obstetrics and Gynecology Hospital
2023-2024
The University of Texas Health Science Center at San Antonio
2012-2024
Anhui University of Traditional Chinese Medicine
2024
The University of Texas Health Science Center at Houston
2010-2024
Ministry of Education of the People's Republic of China
2018-2024
Mays Cancer Center at UT Health San Antonio
2024
National Clinical Research Center for Digestive Diseases
2022-2024
China Medical University
2024
Abstract The heterogeneous nature of hepatocellular carcinoma (HCC) and the lack appropriate biomarkers have hampered patient prognosis treatment stratification. Recently, we identified that a hepatic stem cell marker, epithelial adhesion molecule (EpCAM), may serve as an early biomarker HCC because its expression is highly elevated in premalignant tissues subset HCC. In this study, aimed to identify novel subtypes resemble certain stages liver lineages by searching for EpCAM-coexpressed...
Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene levels arising from cohybridized samples is achieved taking ratios average for individual genes. A novel method image segmentation provided identify target sites and hypothesis test confidence interval developed quantify the significance observed differences in ratios. In particular, probability density ratio maximum-likelihood estimator distribution are...
Sarcomas are a biologically complex group of tumors mesenchymal origin. By using gene expression microarray analysis, we aimed to find clues into the cellular differentiation and oncogenic pathways active in these as well potential biomarkers therapeutic targets. We examined 181 representing 16 classes human bone soft tissue sarcomas on 12,601-feature cDNA microarray. Remarkably, 2,766 probes differentially expressed across this sample set clearly delineated various tumor classes. Several...
Abstract Recent studies have shown that meningeal lymphatic vessels (MLVs), which are located both dorsally and basally beneath the skull, provide a route for draining macromolecules trafficking immune cells from central nervous system (CNS) into cervical lymph nodes (CLNs), thus represent potential therapeutic target treating neurodegenerative neuroinflammatory diseases. However, roles of MLVs in brain tumor drainage immunity remain unexplored. Here we show dorsal undergo extensive...
Abstract The 60 cell lines of the National Cancer Institute Anticancer Drug Screen (NCI-60) constitute most extensively characterized in vitro cancer model. They have been tested for sensitivity to more than 100,000 potential chemotherapy agents and profiled at DNA, RNA, protein, functional, pharmacologic levels. We used NCI-60 three additional develop a database responses cells ionizing radiation. compared clonogenic survival, apoptosis, gene expression response by microarray. Although...
ABSTRACT Human herpesvirus 8 (HHV-8), a gammaherpesvirus implicated in Kaposi's sarcoma, primary effusion lymphoma, and Castleman's disease, encodes several pathogenically important cellular homologs. To define the HHV-8 transcription program, RNA obtained from latently infected body cavity-based lymphoma 1 cells induced to undergo lytic replication was used query custom DNA microarray containing nearly every known viral open reading frame. The patterns of gene expression offer insights into...
Multiple sclerosis (MS) and other T cell-mediated autoimmune diseases develop in individuals carrying a complex susceptibility trait, probably following exposure to various environmental triggers. Owing the presumed weak influence of single genes on disease predisposition recognized genetic heterogeneity disorders humans, candidate gene searches MS have been difficult. In an attempt identify molecular markers indicative status rather than for MS, we show that expression profiling peripheral...
Abstract Purpose: Hepatocellular carcinoma (HCC), a common cancer worldwide, has dismal outcome partly due to the poor identification of early-stage HCC. Currently, one third HCC patients present with low serum α-fetoprotein (AFP) levels, only clinically available diagnostic marker for The aim this study was identify new molecular markers HCC, especially individuals AFP. Experimental Design: We used microarray technique determine expression profiles 218 specimens from either high or From...
The developmental pathway of the neural retina (NR) and retinal pigment epithelium (RPE) has been revealed by extensive research in mice. However, molecular mechanisms underlying development human NR RPE, as well interactions between these two tissues, have not defined. Here, we analyzed 2,421 individual cells from fetal RPE using single-cell RNA sequencing (RNA-seq) technique tightly regulated spatiotemporal gene expression network cells. We identified major cell classes potential crucial...
Being infected by SARS-CoV-2 may cause damage to multiple organs in patients, such as the lung, liver and heart. Angiotensin-converting enzyme 2 (ACE2), reported a receptor, is also expressed human male testes. This suggests potential risk reproductive system. However, characteristics of ACE2-positive cells expression other process-related genes are still worthy further investigation. Here, we performed singlecell RNA seq (scRNA-seq) analysis on 853 embryo primordial germ (PGCs) 2,854 normal...
Healthy renal function depends on normal nephrogenesis during embryonic development. However, a comprehensive gene expression profile of human fetal kidney development remains largely unexplored. Here, using single-cell RNA-sequencing technique, we analyzed >3,000 cells spanning 4 months in utero. Unsupervised analysis identified two progenitor subtypes cap mesenchyme development, suggesting mechanism for sustaining their states. Furthermore, critical transcriptional regulators and signaling...
Mechanisms of implantation such as determination the attachment pole, fetal-maternal communication, and underlying causes failure are largely unexplored. Here, we performed single-cell RNA sequencing on peri-implantation embryos from both humans mice to explore trophectoderm (TE) development embryo-endometrium cross-talk. We found that transcriptomes polar mural TE diverged after hatched zona pellucida in species, with being more mature than TE. The poles show similarities cell cycle...
Abstract Motivation: Expression-based analysis for large families of genes has recently become possible owing to the development cDNA microarrays, which allow simultaneous measurement transcript levels thousands genes. For each spot on a microarray, signals in two channels must be extracted from their backgrounds. This requires algorithms extract arising tagged mRNA hybridized arrayed locations and determine significance signal ratios. Results: paper focuses estimation ratios channels, those...
Abstract BACKGROUND Despite the high prevalence of benign prostatic hyperplasia (BPH) in aging male, little is known regarding etiology this disease. A better understanding molecular BPH would be facilitated by a comprehensive analysis gene expression patterns that are characteristic growth prostate gland. Since genes differentially expressed between and normal tissues likely to reflect underlying pathogenic mechanisms involved development BPH, we performed comparative using cDNA microarray...