A5022316416- Prenatal Screening and Diagnostics
- Epigenetics and DNA Methylation
- Plant Genetic and Mutation Studies
- Neurogenetic and Muscular Disorders Research
- RNA modifications and cancer
- Chronic Lymphocytic Leukemia Research
- Plant Stress Responses and Tolerance
- PI3K/AKT/mTOR signaling in cancer
- Gene expression and cancer classification
- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- GABA and Rice Research
- Reproductive Biology and Fertility
- Genomic variations and chromosomal abnormalities
- Hemoglobinopathies and Related Disorders
- Immunodeficiency and Autoimmune Disorders
- Pluripotent Stem Cells Research
- Genetic Syndromes and Imprinting
- MicroRNA in disease regulation
- Genetic and phenotypic traits in livestock
- Muscle Physiology and Disorders
- Connective tissue disorders research
- Biochemical Analysis and Sensing Techniques
- Light effects on plants
Peking University
2016-2022
Peking University Third Hospital
2016-2022
Beijing Normal University
2005-2022
National Clinical Research Center for Digestive Diseases
2020-2022
Yunnan Forestry Vocational and Technical College
2022
Ministry of Education of the People's Republic of China
2016-2020
Beijing Obstetrics and Gynecology Hospital
2018-2019
Sichuan University
2011-2012
National Institute of Biological Sciences, Beijing
2007
Hebei Normal University
2007
The plant hormone abscisic acid (ABA) regulates many physiological and developmental processes in plants. mechanism of ABA perception at the cell surface is not understood. Here, we report that a G protein–coupled receptor genetically physically interacts with protein α subunit GPA1 to mediate all known responses Arabidopsis . Overexpressing this results an ABA-hypersensitive phenotype. This binds high affinity concentration expected kinetics stereospecificity. binding leads dissociation...
Abstract Extensive epigenetic reprogramming occurs during preimplantation embryo development. However, it remains largely unclear how the drastic contributes to transcriptional regulatory network this period. Here, we develop a single-cell multiomics sequencing technology (scNOMeRe-seq) that enables profiling of genome-wide chromatin accessibility, DNA methylation and RNA expression in same individual cell. We apply method depict map mouse find remodeling facilitates reconstruction genetic...
Mechanisms of implantation such as determination the attachment pole, fetal-maternal communication, and underlying causes failure are largely unexplored. Here, we performed single-cell RNA sequencing on peri-implantation embryos from both humans mice to explore trophectoderm (TE) development embryo-endometrium cross-talk. We found that transcriptomes polar mural TE diverged after hatched zona pellucida in species, with being more mature than TE. The poles show similarities cell cycle...
Abstract STUDY QUESTION Is a novel homozygous phospholipase C zeta (PLCζ), c.1658 G>C; p. R553P mutation in the C2 domain associated with outcomes of recurrent fertilization failure after ICSI? SUMMARY ANSWER PLCζ, G>C led to defective human oocyte activation and failure, while this PLCζ did not compromise concentration, motility chromosome ploidy sperm. WHAT IS KNOWN ALREADY Sperm-specific is now widely considered be physiological stimulus that evokes intracellular calcium...
Chromosomal mosaicism is common throughout human pre- and post-implantation development. However, the incidence characteristics of in blastocyst remain unclear. Concerns confusions still exist regarding interpretation chromosomal on preimplantation genetic testing for aneuploidy (PGT-A) results embryo Here, we aimed to estimate concordance between trophectoderm (TE), inner cell mass (ICM) corresponding embryonic stem cells (hESCs), explore hESCs a single level. The sequencing TE indicated...
Whole-exome sequencing (WES) is widely used to detect genetic mutations that cause Mendelian diseases, and has been successfully applied in combination with preimplantation diagnosis (PGD) avoid the transmission of defects. We investigated 40 nonconsanguineous families unexplained, recurrent fetal malformations (two or more malformed fetuses) from May 2016 December 2018. Using Trio-WES, we identified 32 disease-associated variants (80% positive rate), which were subsequently verified. Known...
This work aimed to identify novel angiotensin-converting-enzyme (ACE) inhibitory peptides from Macadamia integrifolia antimicrobial protein 2 (MiAMP2). The MiAMP2 was hydrolyzed through in silico digestion, and the generated were screened for ACE activity. enzyme digestion results revealed that 18 unreported obtained using AHTPDB BIOPEP-UWM, none thought be toxic based on absorption, distribution, metabolism, excretion (ADMET) prediction. PGPR, RPLY, MNPQR, AAPR predicted exhibit good...
Chemosensory receptors, including odor, taste, and vomeronasal comprise the largest group of G protein–coupled receptors (GPCRs) in mammalian genome. However, little is known about molecular determinants that are critical for detection discrimination ligands by most these receptors. This dearth understanding due part to difficulties preparing functional suitable biochemical biophysical analyses. Here we describe detail two strategies expression purification ligand-binding domain T1R taste...
Abstract Background Haplotyping reveals chromosome blocks inherited from parents to in vitro fertilized (IVF) embryos preimplantation genetic diagnosis (PGD), enabling the observation of transmission disease alleles between generations. However, methods haplotyping that are suitable for single cells limited because a whole genome amplification (WGA) process is performed before sequencing or genotyping PGD, and true haplotype profiles need be constructed based on genotypes can contain many...
Preimplantation genetic diagnosis (PGD) of diseases, combined with human leukocyte antigen (HLA) typing (PGD-HLA), is a useful technique to have healthy offspring that are compatible sibling for hematopoietic stem cells transplantation (HSCT) treat their diseases. Here, we report new strategy using single nucleotide polymorphism (SNP) linkage analysis monogenic disease PGD HLA typing, simultaneously obtain the information chromosomal aneuploidy, target mutations and through low-depth next...
The mutagenic effects of ion implantation on arabidopsis thaliana were analyzed using an amplified fragment length polymorphism (AFLP) fingerprinting. effect the C <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+</sup> was top that N at same fluence. Sequence analysis variant DNA fragments indicated transformation bases appeared to be dominant type mutations induced by implantation. Molecular positional mutagenesis arising from observed....
For patients with pregnancy-induced thalassemia, fetal cord blood or amniotic fluid is invasively collected in the traditional diagnosis and prediction of thalassemia. However, there no specific molecular target thalassemia using DNA from plasma pregnant women.The promoter cell surface adhesion molecule (IGSF4) gene was found to be down-regulated homozygous expression IGSF4 closely associated methylation its promoter. In present study, mass spectrometric sequencing performed MassARRAY detect...
In order to compare the contemporary and genetic variation effect on Arabidopsis thaliana treated with N+ implantation γ-ray radiation, authors did some statistical comparison germinating rate development period, analyzed content of soluble proteins, activity enzymes, isoenzymes profile, along in genome DNA two generations by RAPD. With there was an analogical "saddle model" relationship between doses plant development, enzymes profile. A certain connection might exist similar dose-effect...
Abstract Extensive epigenetic reprogramming occurs during preimplantation embryo development and is accompanied by zygotic genome activation (ZGA) first cell fate specification. Recent studies using single-cell epigenome sequencing techniques have provided global views of the dynamics different layers this period. However, it remains largely unclear how drastic contributes to transcriptional regulatory network. Here, we developed a multiomics technology (scNOMeRe-seq) that enables profiling...
Ion implantation, as a new biophysically mutagenic technique, has shown great potential for crop breeding. By analyzing polymorphisms of genomic DNA through RAPD-based analysis, we compared the frequency and efficiency somatic germ-line mutations Arabidopsis thaliana treated with N+ ion implantation γ-rays radiation. Our data support following conclusions: (1) can induce much wider spectrum than radiation does; (2) Unlike linear correlation between doses their effect in radiation,...
Calcium acts as a universal secondary messenger that transfers developmental cues and stress signals for gene expression adaptive growth. A prior study showed abiotic stresses induce mutually independent cytosolic Ca2+ ([Ca2+]cyt) nucleosolic ([Ca2+]nuc) increases in Arabidopsis thaliana root cells. However, networks deciphering [Ca2+]cyt [Ca2+]nuc signalling pathways remain elusive. Here, using transgenic A. to selectively impair abscisic acid (ABA)- or methyl jasmonate (MeJA)-induced...