A5043806705- Ion Channels and Receptors
- Renal Diseases and Glomerulopathies
- Neuroinflammation and Neurodegeneration Mechanisms
- PARP inhibition in cancer therapy
- Renal and related cancers
- Sirtuins and Resveratrol in Medicine
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Ion Transport and Channel Regulation
- Reproductive Biology and Fertility
- Diet and metabolism studies
- Ovarian function and disorders
- Inflammation biomarkers and pathways
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Blood Pressure and Hypertension Studies
- Nutritional Studies and Diet
- BRCA gene mutations in cancer
- Erythrocyte Function and Pathophysiology
- Neurological Disease Mechanisms and Treatments
- Diet, Metabolism, and Disease
- Atherosclerosis and Cardiovascular Diseases
- Chronic Kidney Disease and Diabetes
- Sperm and Testicular Function
- Tissue Engineering and Regenerative Medicine
Sun Yat-sen Memorial Hospital
2020-2025
Sun Yat-sen University
2020-2025
Broad Institute
2012-2024
Chinese PLA General Hospital
2024
Nanjing Drum Tower Hospital
2024
Nanjing Medical University
2024
Nanjing University
2024
Brigham and Women's Hospital
2015-2021
Harvard University
2012-2021
Jilin Agricultural University
2018-2021
Abstract Human iPSC-derived kidney organoids have the potential to revolutionize discovery, but assessing their consistency and reproducibility across iPSC lines, reducing generation of off-target cells remain an open challenge. Here, we profile four human lines for a total 450,118 single show how organoid composition development are comparable fetal adult kidneys. Although cell classes largely reproducible time points, protocols, replicates, detect variability in proportions between...
Persistently elevated glycolysis in kidney has been demonstrated to promote chronic disease (CKD). However, the underlying mechanism remains largely unclear. Here, we observed that 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), a key glycolytic enzyme, was remarkably induced proximal tubular cells (PTCs) following ischemia-reperfusion injury (IRI) mice, as well multiple etiologies of patients with CKD. PFKFB3 expression positively correlated severity fibrosis. Moreover, CKD...
Abstract Background Macrophages have been shown to contribute renal injury and fibrosis as well repair. Recently, Triggering Receptor Expressed on Myeloid Cells 2 (TREM2)‐positive macrophages play important roles in regulating tissue inflammation However, it remains unclear whether they can mitigate the transition from acute kidney chronic disease (the AKI–CKD transition). Methods The was generated by unilateral ischaemia–reperfusion (UIRI) wild‐type (WT) Trem2 knockout mice. F4/80 magnetic...
Progressive kidney diseases are often associated with scarring of the kidney's filtration unit, a condition called focal segmental glomerulosclerosis (FSGS). This is due to loss podocytes, cells critical for glomerular filtration, and leads proteinuria failure. Inherited forms FSGS caused by Rac1-activating mutations, Rac1 induces TRPC5 ion channel activity cytoskeletal remodeling in podocytes. Whether mediates onset progression unknown. We identified small molecule, AC1903, that...
Transient receptor potential (TRP) melastatin 2 (TRPM2) is a cation channel associated with numerous diseases. It has C-terminal NUDT9 homology (NUDT9H) domain responsible for binding adenosine diphosphate (ADP)-ribose (ADPR), and both ADPR calcium (Ca2+) are required TRPM2 activation. Here we report cryo-electron microscopy structures of human alone, ADPR, Ca2+ NUDT9H forms intra- intersubunit interactions the N-terminal TRPM region (MHR1/2/3) in apo state but undergoes conformational...
Diabetic kidney disease (DKD), the most common cause of failure, is a frequent complication diabetes and obesity, yet to date, treatments halt its progression are lacking. We analyze single-cell transcriptomic profiles from DKD patients two mouse models at multiple time points along progression—high-fat diet (HFD)-fed mice aged 90–100 weeks BTBR ob/ob (a genetic model)—and report an expanding population macrophages with high expression triggering receptor expressed on myeloid cells 2 (TREM2)...
Gibberellins are well known for their growth control function in flower, fruit and seed development, as such, exogenous gibberellic acid (GA) application plays an important role viticulture. Unfortunately, the mechanism by which GA3 acts regulation of these complicated developmental processes grape remains unclear. In present study, we demonstrated that to 'Kyoho' grapevine inflorescences at pre-bloom promoted flower opening, induced coloring abortion. attempt obtain a deeper understanding...
The importance of autophagy in polycystic ovary syndrome (PCOS)-related metabolic disorders is increasingly being recognized, but few studies have investigated the role PCOS. Here, transmission electron microscopy demonstrated that was enhanced ovarian tissue from both humans and rats with Consistent this, granulosa cells PCOS showed increases marker protein light chain 3B (LC3B), whereas levels substrate SQSTM1/p62 were decreased. In addition, ratio LC3-II/LC3-I markedly elevated human...
Do long non-coding RNA (lncRNA) and messenger (mRNA) profiles in follicular fluid from mature immature ovarian follicles differ between healthy women with polycystic ovary syndrome (PCOS)? lncRNA mRNA both differed significantly PCOS patients. Unlike microRNAs, which have been extensively studied, lncRNAs present never sequenced the biological associations of who develop remain largely unknown. A total 18 subjects (8 controls 10 patients) were recruited to participate this study. Recruitment...
AIM:To investigate whether nicotinamide overload plays a role in type 2 diabetes. METHODS:Nicotinamide metabolic patterns of 14 diabetic and non-diabetic subjects were compared using HPLC.Cumulative effects N 1 -methylnicotinamide on glucose metabolism, plasma H2O2 levels tissue adenine dinucleotide (NAD) contents adult Sprague-Dawley rats observed.The human sweat glands rat skin metabolism was investigated sauna burn injury, respectively.
The capsaicin receptor, transient receptor potential vanilloid 1 (TRPV1), acts as a polymodal detector of pain-producing chemical and physical stimuli in sensory neurons. Hyperglycemia hypoxia are two main phenomena diabetes associated with several complications. Although many studies on streptozotocin-induced diabetic rats indicate that early neuropathy is potentiation TRPV1 activity dorsal root ganglion neurons, its underlying mechanism distinctive roles hyperglycemia have not been...
BACKGROUND AND PURPOSE Capsiate is produced by ‘CH‐19 Sweet’ ( Capsicum annuun L.), a non‐pungent cultivar of red pepper. Like capsaicin, capsiate thought to enhance energy metabolism activating the sympathetic nervous system and suppressing inflammation, but underlying mechanisms for this are uncertain. We previously reported that could activate transient receptor potential vanilloid 1 (TRPV1), capsaicin receptor. The purpose present study investigate whether capsinoids other TRP channels....
Mice and rats are the most commonly used animals for preclinical stroke studies, but it is unclear whether targets mechanisms always same across different species. Here, we mapped baseline expression of a chemokine/cytokine subnetwork compared responses after oxygen–glucose deprivation in primary neurons, astrocytes, microglia from mouse, rat, human. Baseline profiles chemokines (CX3CL1, CXCL12, CCL2, CCL3, CXCL10) cytokines (IL-1α, IL-1β, IL-6, IL-10, TNFα) showed significant differences...
Semen samples from men after a short ejaculatory abstinence show improved sperm quality and result in increased pregnancy rates, but the underlying mechanisms remain unclear. Herein, we report that ejaculates (1–3 h) compared with long (3–7 days) periods of showed increases motile count, vitality, normal morphology, acrosome reaction capacity, total antioxidant mitochondrial membrane potential, high DNA stainability, decrease fragmentation index (p, < 0.05). Sperm proteomic analysis 322...
Postoperative cognitive dysfunction is a severe complication of the central nervous system that occurs after anesthesia and surgery, has received attention for its high incidence effect on quality life patients. To date, there are no viable treatment options postoperative dysfunction. The identification hub genes could provide new research directions therapeutic targets future research. identify signaling mechanisms contributing to dysfunction, we first conducted Gene Ontology Kyoto...
Abstract Ultraviolet B (UVB) is one of the most common triggers cutaneous lupus erythematosus (CLE), but mechanism still unclear. Here, we discovered that expression macrophage migration inhibitory factor (MIF), a multipotent cytokine, was significantly increased in keratinocytes patients scRNA-seq data and clinical samples. We further UVB exposure induced MIF releasing from contributed to tissue remodeling inflammation by regulating matrix metalloproteinases (MMPs) pro-inflammatory...
The global increased prevalence of obesity and diabetes occurred after the worldwide spread B-vitamins fortification, in which whether long-term exposure to high level B vitamins plays a role is unknown. Our aim was examine relationships between consumption prevalence.This population based ecological study conducted possible associations macronutrients US using per capita data from Economic Research Service Centers for Disease Control Prevention.The prevalences adult were highly correlated...
Ethyl pyruvate (EP) has been reported to offer a protective effect against ischemic injury through its antiinflammatory action. The nuclear protein high-mobility group box 1 (HMGB1) can activate inflammatory pathways when released from cells. This study was designed investigate the neuroprotective of EP spinal cord and potential role HMGB1 in this process.EP administered at various time points before or after 20 min ischemia male New Zealand rabbits. All animals were sacrificed 72 h...