A5080447501- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Tree Root and Stability Studies
- Plant and Fungal Species Descriptions
- Light effects on plants
- Ecology and Conservation Studies
- Botany, Ecology, and Taxonomy Studies
- Plant-Microbe Interactions and Immunity
- Soybean genetics and cultivation
- Plant Disease Resistance and Genetics
- Polysaccharides and Plant Cell Walls
- Agriculture, Soil, Plant Science
- Plant Gene Expression Analysis
- Plant Taxonomy and Phylogenetics
- Studies on Chitinases and Chitosanases
- CRISPR and Genetic Engineering
- Plant Physiology and Cultivation Studies
- Transgenic Plants and Applications
- Animal Genetics and Reproduction
Huazhong Agricultural University
2023-2024
Shanghai Zhangjiang Laboratory
2024
Kanazawa University
2023
Cold Spring Harbor Laboratory
2010-2022
RIKEN Center for Sustainable Resource Science
2014-2018
Hokkaido University
2013
mRNA migration through plasmodesmata In plants, certain transcription factors are produced in one cell but transported, sometimes as messenger RNA (mRNA), plasmodesmata, channels between neighboring plant cells, where they act. This system helps to manage stem development. Kitagawa et al . now identify part of the machinery that manages this cell-to-cell transport. Transport encoding KNOTTED1 homeobox factor depends on Ribosomal RNA-Processing Protein 44 (AtRRP44A), which is a subunit exosome. —PJH
The shoot stem cell niche, contained within the apical meristem (SAM) is maintained in Arabidopsis by homeodomain protein SHOOT MERISTEMLESS (STM). STM a mobile that traffics cell-to-cell, presumably through plasmodesmata. In maize, homolog KNOTTED1 shows clear differences between mRNA and localization domains SAM. However, are not obviously different Arabidopsis, functional relevance of mobility unknown. Using non-mobile version (2xNLS-YFP-STM), we show required to suppress axillary...
Plant cells are surrounded by a cell wall and do not migrate, which makes the regulation of division orientation crucial for development. Regulatory mechanisms controlling may have contributed to evolution body organization in land plants. The GRAS family transcription factors was transferred horizontally from soil bacteria an algal common ancestor
Ferredoxin:NADP(H) oxidoreductase (FNR) plays a key role in redox metabolism plastids. Whereas leaf FNR (LFNR) is required for photosynthesis, root (RFNR) believed to provide electrons ferredoxin (Fd)-dependent enzymes, including nitrite reductase (NiR) and Fd-glutamine-oxoglutarate aminotransferase (Fd-GOGAT) non-photosynthetic conditions. In some herbal species, however, most nitrate activity located photosynthetic organs, ammonium roots assimilated mainly by Fd-independent NADH-GOGAT....
Abstract Cell-to-cell transport of molecules in plants must be properly regulated for plant growth and development. One specialized mechanism that have evolved involves through plasmodesmata (PD), but when how via PD is among individual cells remains largely unknown, particularly at the single-cell level. Here, we developed a tool quantitatively analyzing cell-to-cell level using protonemata Physcomitrella patens photoconvertible fluorescent protein, Dendra2. In filamentous protonemal...
In multi-cellular organisms, cell-to-cell communication is crucial for adapting to changes in the surrounding environment. plants, plasmodesmata (PD) provide a unique pathway communication. PD interconnect most cells and generate cytoplasmic continuum, allowing trafficking of various micro- macromolecules between cells. This molecular through dynamically regulated by altering permeability dependent on environmental changes, thereby leading an appropriate response stresses; however, how still...
Multicellular organisms use transcripts and proteins as signaling molecules for cell-to-cell communication. Maize KNOTTED1 (KN1) was the first homeodomain transcription factor identified in plants, functions maintaining shoot stem cells. KN1 acts non-cell autonomously, both its messenger RNA (mRNA) protein traffic between cells through intercellular nanochannels called plasmodesmata. mRNA trafficking are regulated by a chaperonin subunit catalytic of exosome, respectively. These studies...
Abstract Cell-to-cell communication is tightly regulated in response to environmental stimuli plants. We previously used a photoconvertible fluorescent protein Dendra2 as model reporter study this process. This experiment revealed that macromolecular trafficking between protonemal cells Physcomitrella patens suppressed abscisic acid (ABA). However, it remains unknown which ABA signaling components contribute suppression and how. Here, we show SUCROSE NON-FERMENTING 1-RELATED PROTEIN KINASE 2...
In plants, the stem cells that form shoot system reside within apical meristem (SAM), which is regulated by feedback signaling between WUSCHEL (WUS) homeobox protein and CLAVATA (CLV) peptides receptors. WUS-CLV can be modulated various endogenous or exogenous factors, such as chromatin state, hormone signaling, reactive oxygen species (ROS) nutrition, leading to a dynamic control of SAM size corresponding activity. Despite these insights, however, knowledge genes still limited, in...
Intercellular communication is fundamental to multicellularity. In plants, plasmodesmata (PD) are membrane-lined channels that connect the cytoplasm of adjacent cells, allowing direct molecular exchange (Fig. 1). growth, development and responses external environment, PD act as traffic controllers, or restricting flux different types molecules downstream processes which they trigger. The European Molecular Biology Organization (EMBO) workshop on 'Intercellular in plant disease' evolved from...
Abstract The localization of a protein provides important information about its biological functions. visualization proteins by immunofluorescence has become an essential approach in cell biology. Here, we describe easy‐to‐follow protocol to localize whole‐mount tissues maize ( Zea mays ) and Arabidopsis. We present the procedure using ear primordia Arabidopsis inflorescence apices as examples, followed tips suggestions for each step. In addition, provide supporting use ImageJ plug‐in...
ABSTRACT In 2020 we suffered from a major global pandemic caused by the SARS-CoV-2 coronavirus. Efforts to contain virus include development of rapid tests and vaccines, which require ready supply viral proteins. Here report production two proteins transient transformation tobacco, leading high expression within three days, subsequent purification intact Such efforts may help develop testing resources alleviate impacts this pandemic.
Calcium (Ca) has various functions in plants, including strengthening cell walls, stabilizing membranes, activating enzymes and signaling, all of which are essential for tissue development growth.Plants that deficient Ca often show symptoms such as reduced growth necrosis young leaves.Since crops Ca-deficiency pose major agricultural problems, the molecular breeding low-Ca tolerant varieties is required.However, genetic basis tolerance still not well understood.One breakthrough approach...