The present study was designed to investigate the role of β-amyloid (Aβ1-42 ) in inducing neuronal pyroptosis and its mechanism. Mice cortical neurons (MCNs) were used this study, LPS + Nigericin induce MCNs (positive control group), Aβ1-42 interfere with MCNs. In addition, propidium iodide (PI) staining examine cell permeability, lactate dehydrogenase (LDH) release assay employed detect cytotoxicity, immunofluorescence (IF) expression level key protein GSDMD, Western blot performed levels...

Abstract Background Study on the expression of miRNA‐22 in serum Alzheimer's disease (AD) patients and mechanism neuroinflammation regulation. Methods ELISA assay was used to detect level inflammatory factors, including interleukin‐1β (IL‐1β), interleukin‐18 (IL‐18), tumor necrosis factor‐α AD patients. TargetScan database luciferase reporter gene indicated that gasdermin D (GSDMD) target miRNA‐22. mimic transfected into microglia, followed by administration LPS Nigericin induce pyroptosis....

Abstract Objective This study was mainly conducted to explore the expression changes of GSDMD and conventional markers (including T‐Tau, Tau181p, Aβ 1–42 ) in cerebrospinal fluid among patients with Alzheimer's disease (AD) vascular dementia (VD), followed by determination role diagnosing identifying AD VD. Methods In this study, 60 VD, AD, 50 healthy controls were enrolled. Lumbar puncture performed collect samples. Patients VD evaluated using Mini‐Mental State Examination (MMSE) scale,...

Abstract M2 macrophages can promote liver cancer metastasis by promoting tumour angiogenesis; however, the mechanism underlying macrophage polarization has not been completely revealed. In this study, we mainly explored long non‐coding RNA‐CRNDE (lncRNA‐CRNDE) in regulating and angiogenesis. The expression of CRNDE was up‐regulated or down‐regulated THP‐1 cells (CRNDE ‐/‐ ‐THP‐1 pcDNA3.1‐CRNDE‐THP‐1). were co‐cultured with cell line H22, induced IL‐4/13 to simulate tumour‐induced...

Aim of this research was to examine the impact paeoniflorin (Pae) in suppressing occurrence ferroptosis individuals with Alzheimer's disease (AD). The study utilized APP/PS1 mice AD as experimental subjects. Following administration Pae, cognitive behaviors were evaluated and key indexes measured, well levels oxidative stress (OS). For in-vitro experiments, Erastin adopted for inducing PC12 cells, level cell detected after Pae treatment. improved ability mice, reduced ferroptosis, decreased...

Abstract Objective To detect the expression of high‐mobility group box protein 1 (HMGB1) and toll‐like receptor 4 (TLR4) their downstream signaling factors—myeloid differentiation factor 88 (MyD88), nuclear kappa B (NF‐κB), tumor necrosis alpha (TNF‐α)—in sera patients with Parkinson's disease (PD) in order to evaluate relationship HMGB1–TLR4 axis PD development progression. Methods The serum HMGB1 TLR4 levels 120 100 healthy volunteers were measured using double‐antibody sandwich ELISA,...

Piperine (PIP), the main active ingredient in pepper, belongs to cinnamamide alkaloid. PIP has been found have functions, including anti-oxidation, immune regulation, anti-tumour and promotion of drug metabolism. The present study was mainly designed reveal effect against gastric cancer relevant mechanism. In brief, undifferentiated human cell HGC-27 used, which treated with different concentrations PIP. As a result, could inhibit proliferation induce apoptosis cells dose-dependent manner....

Parkinson's disease (PD) is a common degenerative of the central nervous system (CNS). The main pathological change apoptosis dopaminergic neurons in substantia nigra pars compacta (SNPc), thereby leading to dopamine reduction nigral striatum. 6-Hydroxydopamine (6-OHDA), neurotoxic substance, mediates and causes Parkinson-like symptoms mice.

This work aimed to investigate the role of helper T cell 1 (Th1) in chronic colitis and its immunoregulatory mechanism. The proportions Th1 Th2, levels related cytokines tissues from patients with inflammatory bowel disease (IBD; ulcerative colitis+Crohn's disease, UC+CD) were detected. DSS was used induce mouse model IBD; thereafter, cells induced vitro amplified before they injected intraperitoneally. Later, changes life state body weight mice observed, proportion M1 macrophages mucosal...

Abstract This study aimed to investigate the role of protein kinase HIPK2 in depression and its associated mechanism. The chronic unpredictable mild stress (CUSM) model was constructed simulate mice with detect mouse behaviors. Moreover, by using microglial cells BV2 as model. After conditional knockdown HIPK2, depressive behavior disorder improved, meanwhile, neuroinflammation alleviated, M1 cell proportion reduced. Similar results were obtained after applying inhibitor tBID or ASO‐HIPK2...

Background: An emerging theme in the study of pathophysiology persistent pain is role reactive oxygen species (ROS). In present study, we examined hypothesis that exogenous supply antioxidant drugs during peri‐reperfusion would attenuate induced by ischemia/reperfusion (IR) injury. We investigated analgesic effects three antioxidants administered using an animal model complex regional syndrome‐type I consisting chronic post‐ischemia (CPIP) hind paw. Methods: Application a tight‐fitting...

Abstract In this study, FoxO1 transgenic mice (transgenic, FoxO1‐Tg) and C57BL/6 wild‐type (wild‐type, FoxO1‐WT) were used to establish chronic colitis by drinking water containing dextran sulphate sodium (DSS). Afterwards, we observed the life changes in assessed pathological H&E tissue staining. addition, TLR4/MyD88/MD2‐NF‐κB inflammatory signals detected. As a result, under DSS treatment, activation level of signal was higher FoxO1‐Tg than that FoxO1‐WT mice. Meanwhile, intestinal...

Triptolide (TRI) is an active diterpenoid lactone compound isolated from Tripterygium wilfordii,We focused on investigating the effect and mechanism of liver injury.The toxic dose (LD50 = 100 μM) TRI Kupffer cells was explored, network pharmacological analysis performed to identify Caspase-3 as target TRI-induced injury. Regarding pyroptosis research, we examined level in cells, including inflammatory cytokine detection, protein assay, microscopic cell observation LDH toxicity test. The...

Abstract Antrodia Camphorata Polysaccharide (ACP) refers to a kind of polysaccharide extracted from the natural porous fungus camphorata. This study investigated mechanism action ACP in protecting liver. The results showed that suppressed LPS‐induced KC cell activation, reduced expression inflammatory factors, increased SOD level and ROS expression. In addition, N‐acetylcysteine (NAC) was adopted for pre‐treatment suppress ROS. indicated NAC synergistically exerted its effect with ACP,...

Abstract We mainly study the role and regulatory mechanism of double‐negative T cells (DNTs) in Alzheimer's disease (AD). The mice splenic DNTs were separated amplified by Rosettesep antibody adsorption method Easysep magnetic activated cell sorting. intraperitoneally injected into APP/PS1‐AD model, which was found to aggravate cognitive impairment mice. secreted tumor necrosis factor α (TNF‐α) promote activation NLRP3 M1 polarization microglial cells, silencing with small interfering RNA...