A5032137969- DNA Repair Mechanisms
- PARP inhibition in cancer therapy
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Immune Cell Function and Interaction
- Xenotransplantation and immune response
- T-cell and B-cell Immunology
- Genomics and Chromatin Dynamics
- Carcinogens and Genotoxicity Assessment
- Effects and risks of endocrine disrupting chemicals
- Pluripotent Stem Cells Research
- Renal and related cancers
- Polyomavirus and related diseases
- Cytomegalovirus and herpesvirus research
- Toxic Organic Pollutants Impact
- Cardiac, Anesthesia and Surgical Outcomes
Norwegian University of Science and Technology
2018-2023
National University of San Marcos
2013-2023
Science for Life Laboratory
2020-2022
Karolinska Institutet
2020-2022
St Olav's University Hospital
2018-2020
University of Bergen
2018
Pontificia Universidad Católica de Chile
2017
Oxidative DNA damage is recognized by 8-oxoguanine (8-oxoG) glycosylase 1 (OGG1), which excises 8-oxoG, leaving a substrate for apurinic endonuclease (APE1) and initiating repair. Here, we describe small molecule (TH10785) that interacts with the phenylalanine-319 glycine-42 amino acids of OGG1, increases enzyme activity 10-fold, generates previously undescribed β,δ-lyase enzymatic function. TH10785 controls catalytic mediated nitrogen base within its molecular structure. In cells, OGG1...
Endocrine-disrupting chemicals (EDCs) generate reproductive dysfunctions affecting the biosynthesis of steroid hormones and genes steroidogenic pathway. EDCs effects are mainly reported as a result exposure to single compounds. However, humans environmentally exposed mixture EDCs. Herein, we assess chronic alkylphenols phthalates versus in mouse testes histology steroidogenesis. Pregnant mice were through drinking water to: 0.3 mg/kg-body weight (BW)/d each phthalate (bis (2-ethylhexyl)...
Classical non-homologous end joining (NHEJ) is a molecular pathway that detects, processes, and ligates DNA double-strand breaks (DSBs) throughout the cell cycle. Mutations in several NHEJ genes result neurological abnormalities immunodeficiency both humans mice. The required for V(D)J recombination developing B T lymphocytes, class switch mature cells. Ku heterodimer formed by Ku70 Ku80 recognizes DSBs facilitates recruitment of accessory factors (e.g., DNA-PKcs, Artemis, Paxx Mri/Cyren)...
DNA double-strand breaks (DSBs) trigger the Ataxia telangiectasia mutated (ATM)-dependent damage response (DDR), which consists of histone H2AX, MDC1, RNF168, 53BP1, PTIP, RIF1, Rev7, and Shieldin. Early stages B T lymphocyte development are dependent on recombination activating gene (RAG)-induced DSBs that form basis for further V(D)J recombination. Non-homologous end joining (NHEJ) pathway factors recognize, process, ligate DSBs. Based numerous loss-of-function studies, DDR were thought to...
Non-homologous end-joining (NHEJ) is a DNA repair pathway required to detect, process, and ligate double-stranded breaks (DSBs) throughout the cell cycle.The NHEJ necessary for V(D)J recombination in developing B T lymphocytes.During NHEJ, Ku70 Ku80 form heterodimer that recognizes DSBs promotes recruitment function of downstream factors PAXX, MRI, DNA-PKcs, Artemis, XLF, XRCC4, LIG4.Mutations several known genes result severe combined immunodeficiency (SCID).Inactivation Mri, Paxx or Xlf...
DNA double-strand breaks (DSBs) trigger the Ataxia telangiectasia mutated (ATM)-dependent damage response (DDR), which consists of histone H2AX, MDC1, RNF168, 53BP1, PTIP, RIF1, Rev7, and Shieldin. Early stages B T lymphocyte development are dependent on recombination activating gene (RAG)-induced DSBs that form basis for further V(D)J recombination. Non-homologous end joining (NHEJ) pathway factors recognize, process, ligate DSBs. Based numerous loss-of-function studies, DDR were thought to...
Classical non-homologous end joining (NHEJ) is a molecular pathway that detects, processes and ligates DNA double-strand breaks (DSBs) throughout the cell cycle. Mutations in several NHEJ genes result neurological abnormalities immunodeficiency both humans mice. The required for V(D)J recombination developing B T lymphocytes, class switch mature cells. Ku heterodimer formed by Ku70 Ku80 recognizes DSBs facilitates recruitment of accessory factors (e.g., DNA-PKcs, Artemis, Paxx Mri/Cyren)...
The identification system of spermatogonial stem cell (SSC) was established in alpaca using the molecular expression as well reactivity pattern to Dolichos biflorus agglutinin (DBA) by flow cytometry. Twenty-four testicles with their epididymis were recovered from adult alpacas at slaughterhouse Huancavelica-Perú. Samples transported Laboratory Reproductive Physiology Universidad Nacional Mayor de San Marcos. Testes selected for our study when progressive motility epididymal spermatozoa...
The non-homologous end joining (NHEJ) DNA repair pathway is the main mechanism to double strand breaks (DSBs) throughout whole cell cycle. During NHEJ, core Ku70 and Ku80 subunits form Ku heterodimer; binds DSBs promotes recruitment of accessory factors (e.g., DNA-PKcs, PAXX, Mri) downstream (XLF, Lig4 XRCC4). are induced during V(D)J recombination in developing B T lymphocytes increase repertoire receptors. Furthermore, generated class switch (CSR) mature lymphocytes, providing distinct...
Non-homologous end-joining (NHEJ) is a DNA repair pathway required to detect, process, and ligate double-stranded breaks (DSBs) throughout the cell cycle. The NHEJ necessary for V(D)J recombination in developing B T lymphocytes. During NHEJ, Ku70 Ku80 form heterodimer that recognizes DSBs promotes recruitment function of downstream factors PAXX, MRI, DNA-PKcs, Artemis, XLF, XRCC4, LIG4. Mutations several known genes result severe combined immunodeficiency (SCID). Inactivation Mri, Paxx or...
Spermatogonial stem cell (SSC) is known for its self-renewal capacity. We have studied the in vitro proliferation of isolated SSC from adult alpaca (Vicugna pacos) testes. A total 107 samples were evaluated which 31 at baseline, 36 cultivated DMEM and 40 STEMPRO media. Half was analysed after 14 days, rest 21 days. Round subpopulations identified with FITC-DBA by flow cytometry: strongly positive DBA (sDBA+) as SSC, weakly (wDBA+) early differentiation negative differentiated. At beginning,...
Abstract Non-homologous end-joining (NHEJ) is a DNA repair pathway required to detect, process, and ligate double-stranded breaks (DSBs) throughout the cell cycle. The NHEJ necessary for V(D)J recombination in developing B T lymphocytes. During NHEJ, Ku70 Ku80 form heterodimer that recognizes DSBs promotes recruitment function of downstream factors PAXX, MRI, DNA-PKcs, Artemis, XLF, XRCC4, LIG4. Mutations several known genes result severe combined immunodeficiency (SCID). Inactivation Mri,...
La evaluación de la eficiencia es una herramienta útil para correcta toma decisiones en los centros salud. Esta incluye conocer variabilidad y confiabilidad tiempos procedimientos médicos quirúrgicos registrados. Objetivos: Determinar registrados procesos operatorios cesáreas. Diseño: Estudio transversal. Institución: Clínica Centenario Peruano Japonesa. Participantes: Registros pacientes con Intervenciones: A partir del registro operatorio las historias clínicas se obtuvo datos operatorios,...